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|本期目录/Table of Contents|

替米沙坦上调胰岛素抵抗大鼠心肌脂联素及其受体的表达

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第2期
页码:
141-145
栏目:
基础研究
出版日期:
2010-12-10

文章信息/Info

Title:
Telmisartan upregulates expression of myocardial adiponectin and receptor in insulin-resistant rats
作者:
郭春艳; 李虹伟; 沈潞华; 赵树梅
首都医科大学附属北京友谊医院心内科，北京 100050
Author(s):
GUO Chun-yan;  LI Hong-wei;  SHEN Lu-hua;  ZHAO Shu-mei
Department of Cardiology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China
关键词:
脂联素; 心肌; 胰岛素抵抗; 受体; 替米沙坦
Keywords:
adiponectin;  cardiomyocyte;  insulin resistance;  receptor;  telmisartan
分类号:
R915
DOI:
-
文献标识码:
A
摘要:
目的: 探讨胰岛素抵抗(IR)大鼠心肌脂联素(APN)及其受体(AdipoR1和AdipoR2)表达改变和替米沙坦(TMST)的影响。方法： 雄性SD大鼠50只随机选择10只作为对照组（用普通饲料喂养8周），其余40只大鼠用高果糖饮食喂养4周建立IR模型。从IR大鼠中随机选择10只作为IR组（用生理盐水灌胃4周，继续用高果糖饮食喂养）；另选10只作为TMST组［用替米沙坦5 mg/(kg·d)灌胃4周，继续用高果糖饮食喂养］。所有大鼠均于喂养的第8周末，空腹采血，分别采用ELISA法和放射免疫法测定血清APN和肿瘤坏死因子-α（TNF-α）的水平。采用Western blot法测定各组大鼠心肌组织中APN、AdipoR1、AdipoR2、过氧化物酶体增殖物激活受体-γ（PPARγ）及TNF-α蛋白的表达。结果: 实验第8周末，与对照组相比，IR组血清APN的水平降低 （P<0.01）而血清TNF-α的水平升高（P<0.01）。大鼠心肌中APN、AdipoR1及PPAR-γ蛋白的表达降低（P<0.01）；心肌中TNF-α蛋白的表达增高（P<0.01），而心肌中AdipoR2蛋白的表达无明显变化。与IR组大鼠相比，TMST组大鼠血清APN增高（P<0.05）而血清TNF-α水平降低（P<0.05），心肌APN、AdipoR1及PPAR-γ蛋白的表达增高（P<0.05）；心肌中TNF-α蛋白的表达降低（P<0.05），而心肌中AdipoR2蛋白的表达无明显变化。结论： IR大鼠心肌中APN及AdipoR1及PPARγ蛋白的表达降低；心肌中TNF-α蛋白的表达增高。TMST可通过激活PPARγ途径，增加循环中APN的水平，特别是激活心肌组织中的PPARγ，减轻炎症，提高APN及其受体的表达。
Abstract:
AIM: To explore expression of myocardial adiponectin and its receptors in insulin-resistant (IR) rats and to study the effect of telmisartan (TMST). METHODS: Fifty male Sprague Dawley rats were included in the study, ten of whom were put in group C and were fed with ordinary diet for 8 weeks. The other 40 rats were fed with a high-fructose diet for 4 weeks to induce IR rat model. Ten IR rats were selected as IR group and were fed with high-fructose diet and treated with 0.9% saline for the next 4 weeks. Another 10 IR rats were selected as TMST group and were fed with high-fructose diet and treated with telmisartan 5 mg/(kg·day) for the next 4 weeks. After 8 weeks, blood samples were obtained and APN and TNF-α were measured. Protein expressions of myocardial APN, AdipoR1, AdipoR2, PPARγ and TNF-α were detected by Western blot. RESULTS: Compared with those in group C, serum APN decreased and TNF-α level increased in IR group. Compared with those in IR group, serum APN increased and serum TNF-α level decreased in TMST group. Protein expressions of myocardial APN, AdipoR1 and PPARγ were attenuated with enhanced TNF-α in IR group compared with those in group C. Protein expressions of myocardial APN, AdipoR1 and PPARγ were enhanced with attenuated TNF-α in TMST group compared with those in IR group. CONCLUSION: In IR rats, myocardial protein expressions of APN, AdipoR1 and PPARγ decreased and myocardial protein expressions of TNF-α increased. TMST can increase the myocardial expressions of adiponectin AdipoR1 and PPARγ and reduce inflammation.

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备注/Memo

备注/Memo:
收稿日期：2010-04-15.基金项目：北京市自然科学基金项目资助（7082027） 通讯作者：李虹伟，主任医师，主要从事心血管疾病及代谢综合征防治的研究Email：mcw19656@yahoo.com.cn 作者简介：郭春艳，主治医师，博士Email：cardiovas@hotmail.com
更新日期/Last Update: 2010-12-10