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|本期目录/Table of Contents|

血管紧张素Ⅱ对巨噬细胞和泡沫细胞ACAT-1及PPAR-γ表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第2期
页码:
169-172
栏目:
基础研究
出版日期:
2010-12-10

文章信息/Info

Title:
Effects of angiotensin II on expression of acyl-coenzyme A: cholesterol acyltransferase-1 and peroxisome proliferator-activated receptor-γ in macrophages and foam cells
作者:
马志强1; 刘力1; 成蓓2; 王志权1
1.武汉解放军第161医院心内科，湖北 武汉 430010;2.华中科技大学同济医学院附属协和医院综合科,湖北 武汉 430022
Author(s):
MA Zhi-qiang1;  LIU Li1;  CHENG Bei2;  WANG Zhi-quan1
Department of Cardiology, PLA 161 Hospital, Wuhan 430010, Hubei, China
关键词:
胆固醇酰基转移酶-1; 巨噬细胞; 泡沫细胞; 血管紧张素Ⅱ; 动脉粥样硬化; 过氧化体增殖物激活型受体γ
Keywords:
atherosclerosis;  angiotensin II;  cholesterol acyltransferase-1;  peroxisome proliferator activated receptor-γ;  foam cell;  macrophage
分类号:
R972.5
DOI:
-
文献标识码:
A
摘要:
目的: 观察血管紧张素Ⅱ（AngⅡ）对巨噬细胞和泡沫细胞过氧化体增殖物激活型受体γ(PPAR-γ)和酰基辅酶A：胆固醇酰基转移酶-1 (ACAT-1)表达的影响。方法： 将单核细胞株THP-1与160 nmol/L佛波酯(PMA)孵育48 h，使之分化为巨噬细胞，继以100 mg/L氧化型低密度脂蛋白(ox-LDL)诱导巨噬细胞分化为泡沫细胞。将巨噬细胞和泡沫细胞用1×10-6 mol/L AngⅡ孵育48 h。运用RT-PCR和Western blot分别检测PPAR-γ mRNA、ACAT-1 mRNA及其蛋白的表达水平。结果： 单核细胞在分化为巨噬细胞和泡沫细胞的过程中，PPAR-γ的表达明显降低（P<0.05），ACAT-1的表达显著增强（P<0.05）；经AngⅡ干预后，巨噬细胞和泡沫细胞PPAR-γ的表达进一步降低（P<0.05，P<0.05），而ACAT-1的表达更加增强（P<0.05，P<0.05）。结论： AngⅡ不但可上调巨噬细胞和泡沫细胞中ACAT-1的表达，而且可抑制PPAR-γ的表达。
Abstract:
AIM: To investigate the effects of angiotensin II (Ang II) on expression of acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) and peroxisome proliferator-activated receptor-γ (PPAR-γ) in macrophages and foam cells. METHODS: After human THP-1 cells were incubated with 160 nmol/L PMA for 48 h to differentiate into macrophages, the macrophages were cultured with 100 mg/L oxidized LDL for 24 h to differentiate into foam cells. Macrophages and foam cells were incubated with 1×10-6 mol/L Ang II for 48 h. ACAT-1 mRNA and PPAR-γ mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) and protein levels of ACAT-1 and PPAR-γ were measured by Western blot. RESULTS: ACAT-1 mRNA and protein levels were upregulated significantly (P<0.05) during differentiation from monocytes into macrophages and differentiation from macrophages into foam cells, with the PPAR-γ mRNA and protein levels significantly downregulated (P<0.05). When treated with Ang II, the ACAT-1 mRNA and protein levels of macrophages and foam cells were up-regulated more significantly (P<0.05, P<0.05) and their PPAR-γ mRNA and protein levels were more significantly downregulated (P<0.05, P<0.05). CONCULUSION: Ang II not only significantly promotes ACAT-1 expression but also inhibits PPAR-γ expression during the monocytes-macrophages differentiation.

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备注/Memo

备注/Memo:
收稿日期：2010-05-05.作者简介：马志强,副主任医师,博士Email:mzqy@tom.com
更新日期/Last Update: 2010-12-10