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反复力竭性运动后大鼠血清cTnI和心肌组织形态学的改变

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第2期
页码:
197-200
栏目:
基础研究
出版日期:
2010-12-10

文章信息/Info

Title:
Changes of serum cTnI and myocardium histomorphology after repeated exhaustive exercise in rats
作者:
王福文1; 胡志力1; 赵敬国2; 李杰1
1.山东省医学科学院药物研究所、山东省现代医用药物与技术重点实验室，山东 济南 250062；2.山东师范大学体育学院运动人体科学实验室，山东 济南 250014
Author(s):
WANG Fu-wen1;  HU Zhi-li1;  ZHAO Jing-guo2;  LI Jie1
1.Institute of Materia Medica, Shandong Academy of Medical Sciences, Key Laboratory for Modern Medicine and Technology of Shandong Province, Jinan 250062, Shandong, China; 2.Human Movement Science Laboratory, Physical Education College, Shandong Normal University, Jinan 250014, Shandong, China
关键词:
力竭性运动; 心肌肌钙蛋白I; 心肌损伤; 病理组织学改变; 大鼠
Keywords:
exhaustive exercise;  cardiac troponin I;  myocardial injury;  pathological histology;  rats
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的: 观察反复力竭性游泳运动后，不同时相大鼠血清心肌肌钙蛋白I（cTnI）与心肌组织形态学的动态改变，以评价过度运动后恢复期是否存在延迟性心肌损伤？方法： 采用经典的Thomas的方法，通过力竭性游泳运动建立运动性心肌损伤实验动物模型。将无训练的87只Wistar雄性大鼠随机分为安静对照组和力竭性运动后即刻组、3 h组、6 h组、12 h组、24 h组、48 h组和96 h组。分别于末次运动结束后即刻、3、6、12、24、48和96 h，采血分离血清检测cTnI，并进行心肌组织的病理学检查。结果： ①与安静对照组比较，力竭性运动后即刻、6 h和48 h大鼠血清cTnI的含量明显增加(P<0.05和P<0.01)，其中运动后6 h和48 h分别达高峰，运动后3、12、24 h仍保持在较高水平，运动后96 h基本恢复运动前的水平。②经1周反复力竭性运动，光镜下可见运动后不同时相大鼠心肌细胞均有不同程度的损伤，运动后48 h最严重，96 h有所减轻。结论： 长期过度运动和/或力竭性运动后，心肌组织发生病理性改变，且具有延迟性加重现象，即存在延迟性心肌损伤。
Abstract:
AIM: To study the dynamic changes of serum cTnI and myocardium histomorphology in different time periods after exhaustive exercise in rats in order to evaluate delayed-onset myocardial damage during recovery phase after exercise. METHODS: Animal models of exercise-induced myocardial injury were established according to Thomas’s method. Healthy untrained 87 male Wistar rats were selected and randomly divided into one sedentary control group (n=10) and seven exhaustive exercise groups according to times of recovery phase after exercise (immediately after, 3, 6, 12, 24, 48 and 96-h groups with 11 rats in each group). Blood samples were obtained and cTnI in serum was measured. Changes of myocardial histopathology were examined immediately after, 3, 6, 12, 24, 48 and 96 h after exercise. RESULTS: Compared with that in sedentary control group, the level of serum cTnI was significantly increased at immediately after, 6 and 48 h after repeated exhaustive exercise (P<0.05 and P<0.01), peaked at 6 h and 48 h, maintained the higher level at 3, 12 and 24 h and basically recovered at 96 h post-exercise. After 1 week consecutive daily exhaustive exercise, myocardial injuries of different degrees were observed at immediately after, 3, 6, 12, 24, 48 and 96 h after exhaustive exercise under light microscope. Myocardial injury was most severe at 48 h and was alleviated at 96 h post-exercise. CONCLUSION: After long-term excessive exercise and/or exhaustive exercise, the pathological changes in myocardium tissue occurred in a time-dependent manner. Similar to skeletal muscle, delayed-onset myocardial injury is also induced by exhaustive exercise.

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备注/Memo

备注/Memo:
收稿日期：2010-03-17.基金项目：山东省自然科学基金项目（ZR2009CM089）；山东省教育厅科技计划项目（J07YE09） 作者简介：王福文，研究员，硕士Email：wangfuwww@tom.com
更新日期/Last Update: 2010-12-10