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银杏内酯B减轻血管内皮细胞缺氧/复氧损伤的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第3期
页码:
318-321
栏目:
基础研究
出版日期:
2011-05-12

文章信息/Info

Title:
Anti-injury effect of ginkgolide B on vascular endothelial cells induced by hypoxia/reoxygenation
作者:
张荣怀1; 房海英1; 陈金凤1; 王琳1; 程珂1; 郭海涛2
第四军医大学：1．西京医院老年病科，2．生理学教研室，陕西 西安，710032
Author(s):
ZHANG Rong-huai1;  FANG Hai-ying1;  CHEN Jin-feng1;  WANG Lin1;  CHENG Ke1;  GUO Hai-tao2
1.Department of Geriatrics, Xijing Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
银杏内酯B; 缺氧/复氧; 人脐静脉皮细胞; 内皮型一氧化氮合成酶; 一氧化氮
Keywords:
ginkgolide B;  hypoxia/reoxygenation;  human umbilical vein endothelial cells;  endothelial nitric oxide synthase;  nitric oxide
分类号:
R282.71
DOI:
-
文献标识码:
A
摘要:
目的:探讨银杏内酯B(GB)减轻缺氧/复氧(H/R)所致血管内皮细胞损伤的作用和机制。方法： 将体外培养的人脐静脉内皮细胞分为5个组，即空白对照组（不加任何处理）、GB组（100 μmol/L）、H/R组（缺氧2 h/复氧24 h）、GB+H/R组及GB+H/R+ N-硝基-L-精氨酸甲酯（L-NAME，NOS抑制剂）组。用3-(4，5-二甲基噻唑-2)-2，5-二苯基四氮唑溴盐（MTT）比色法测定内皮细胞的存活率(%)。用乳酸脱氢酶（LDH）测试盒和一氧化氮（NO）测试盒分别检测细胞培养上清液中LDH和NO的含量。用Western blot法检测培养的人脐静脉内皮细胞中内皮型一氧化氮合成酶（eNOS）表达的水平。结果： MTT比色法的结果显示，H/R可显著降低内皮细胞的存活率(%)，增加LDH的含量，降低NO的生成量（与空白对照组比较，P<0.01）；而GB则可逆转上述效应，但GB的作用又可被L-NAME所抑制（与GB+H/R组比较，P<0.01）。GB可以显著提高H/R作用后血管内皮细胞中eNOS表达的水平（与H/R组比较，P<0.01），但是该效应不被L-NAME阻断。结论： GB可显著减轻H/R对内皮细胞的损伤，其作用是通过eNOS-NO途径实现的。
Abstract:
AIM:To investigate the protective effects of ginkgolide B (GB) on human umbilical vein endothelial cells (HUVECs) induced by hypoxia/reoxygenation (H/R) injury and the underlying mechanisms. METHODS: Cultured HUVECs were divided into five groups: control group, GB group (100 mol/L), H/R group (hypoxia 2 h/reoxygenation 24 h), GB+H/R group and GB+H/R+NG-nitro-L-arginine methyl ester (L-NAME) group. Cell viability was measured by 3-［4,5-dimethylthiazol-2-yl］-2,5-diphenyltetrazolium bromide (MTT) colorimetric method and lactate dehydrogenase (LDH), nitric oxide (NO) in the supernatant fluid of cultured cells was detected by spectrophotometer. Endothelial nitric oxide synthase (eNOS) protein was detected by Western blot. RESULTS: H/R reduced the MTT OD value, increased LDH content and decreased NO level significantly (P<0.01 vs. control group), but GB reversed these effects on HUVECs. These GB effects were inhibited by L-NAME (P<0.01 vs. GB+H/R group). GB increased H/R-reduced content of eNOS (P<0.01 vs. H/R group), but this effect was not inhibited by L-NAME. CONCLUSION: GB has some anti-injury effects on HUVECs induced by H/R via enhancing the levels of eNOS and NO.

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备注/Memo

备注/Memo:
收稿日期：2009-09-06.基金项目：陕西省中医管理局中药科研课题资助项目(2009-JC49) 通讯作者：郭海涛，讲师，主要从事缺血性心血管病的研究Email:haitaoguo@fmmu.edu.cn 作者简介：张荣怀，博士生Email:rhzhang@fmmu.edu.cn
更新日期/Last Update: 2011-03-17