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胡椒碱减轻兔原代左心房肌细胞氧化应激损伤的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第4期
页码:
426
栏目:
基础研究
出版日期:
2011-08-25

文章信息/Info

Title:
Protective effects of piperine against oxidative stress in rabbit primary left atrial cells
作者:
田苗1; 刘屏2; 王字玲3; 官媛1 ; 刘岩1; 王玉堂1; 单兆亮1
解放军总医院：1.心内科，2.药理研究室，北京 100853；3.军事医学科学院野战输血研究所，北京 100850
Author(s):
TIAN Miao1;  LIU Ping2;  WANG Zi-ling3;  GUAN Yuan1;  LIU yan1;  WANG Yu-tang1;  SHAN Zhao-liang1
1.Department of Cardiology, 2.Department of Clinical Pharmacology and Pharmacy, PLA General Hospital, Beijing 100853, China; 3.Institute of Blood Transfusion Medicine, Academy of Military Medical Sciences, Beijing 100850, China
关键词:
胡椒碱; 心房; 原代细胞; 氧化应激; 过氧化氢
Keywords:
piperine;  atrium;  primary cell;  oxidative stress;  hydrogen peroxide
分类号:
R282.71
DOI:
61-1268/R.20110503.1149.003
文献标识码:
A
摘要:
目的:利用低浓度过氧化氢（H2O2）建立兔原代左心房肌细胞氧化损伤模型，探讨胡椒碱减轻氧化应激损伤的保护作用。方法： 将18只新西兰白兔随机分为3组，每组6只：即正常对照(NC)组、H2O2组和胡椒碱组，均分别进行左心房肌细胞的原代培养。NC组对培养的心房细胞直接进行检测，H2O2组直接在培养的原代心房肌细胞中加入终浓度为100 μmol/L的H2O2培养2 h，胡椒碱组以7×10-6 mol/L浓度的胡椒碱处理细胞1 h之后，加入终浓度为100 μmol/L的H2O2共同培养2 h。检测氧化和抗氧化指标的变化。MTT法检测三组原代细胞活力，黄嘌呤氧化酶法检测超氧化物歧化酶（SOD）活力、比色法检测丙二醛（MDA）含量及还原型谷胱甘肽（GSH）含量，Fura-2 AM检测细胞内钙离子浓度，RT-PCR对线粒体mRNA进行定量分析。结果： 与NC组相比较，H2O2组细胞的活力、SOD的活力及GSH的含量明显下降（P<0.05）； MDA的含量、钙离子浓度和线粒体mRNA的表达均明显增加（P<0.05）。胡椒碱组和H2O2组比较，上述指标均有显著改善（P<0.05）。结论： 胡椒碱能够在氧自由基的产生清除等环节，减轻兔原代左心房肌细胞的氧化应激损伤。
Abstract:
AIM:To evaluate the injury caused by low concentration hydrogen peroxide (H2O2) in primary left atrial cells and the protective effect of piperine in rabbits. METHODS: Eighteen rabbits were randomly divided into three groups: normal control (NC) group, H2O2 group and piperine group. Left atrial cells were primarily cultured. In H2O2 group, 100 μmol/L H2O2 was added into culture medium for 2 hours. In piperine group, 7×10-6 mol/L piperine was added for 1 hour and then 100 μmol/L H2O2 was added for 2 hours. The parameters of oxidation and antioxidation were measured. RESULTS: In H2O2 group, cell viability, activity of superoxide dismutase (SOD) and content of glutathione hormone (GSH) decreased (P<0.05). But content of malondialdehyd (MDA), intracellular free calcium concentration and expression of mitochondrial mRNA increased obviously (P<0.05). Compared with those in the H2O2 group, these parameters improved significantly (P<0.05) in the piperine group. CONCLUSIONS: Piperine protects the oxidative stress induced by H2O2 in rabbit primary left atrial cells.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2011-01-29.基金项目：国家自然科学基金项目资助（30772886） 通讯作者：单兆亮，主任医师，主要从事心脏电生理研究Email：shanzl301@sina.com 作者简介：田苗，住院医师，硕士生Email：tianmiao8108@sina.com
更新日期/Last Update: 2011-06-02