贝前列素钠预处理对兔心肌缺血/再灌注损伤的早期拮抗作用(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2011年第4期
- 页码:
- 434
- 栏目:
- 基础研究
- 出版日期:
- 2011-08-25
文章信息/Info
- Title:
- Early antagonism of beraprost sodium pretreatment on ischemic/reperfusion injury in rabbit myocardium
- Author(s):
- YANG Xue; WANG Xiao-yun; MU Zhi-rui; LU Ying-jie; ZHANG Xu-chang
- Department of Cardiology, Fourth Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China
- 分类号:
- R542.2
- DOI:
- 61-1268/R.20110503.1152.005
- 文献标识码:
- A
- 摘要:
- 目的:探讨贝前列素钠（beraprost sodium，BPS）预处理对兔心肌缺血/再灌注(I/R)损伤的早期拮抗作用。方法： 将36只日本大耳白兔随机分为3组，即A组（假手术组）、B组（I/R组：缺血40 min后，再灌注90 min）和C组（BPS预处理组：以15 μg/kg，2次/d，［1］连续喂养4周，最后1次喂养后3 h内进行I/R）。以上3组均开胸暴露心脏，A组在左冠状动脉左室支处穿线不结扎，观察130 min结束实验；B组及C组开胸后结扎左冠状动脉左室支40 min，心电图若出现Ⅱ导联ST段升高及结扎周围心肌的颜色变紫或变深显示已形成心肌梗死(MI)模型。然后进行以下检测：①再灌注90 min后，随机选取6只日本大耳白兔，立即取结扎点下损伤的心肌组织，用100 g/L福尔马林固定后，部分进行HE染色在光镜下观察心肌形态学改变；部分用免疫组化染色法检测心肌细胞中Bax、Bcl-2的表达。②再灌注4 h后，取右心房（或股静脉）血应用电化学发光免疫法检测血浆中心肌中肌酸激酶(CK-MB)的变化。结果： ①HE染色显示，A组的心肌结构正常，B组的心肌损伤较严重，C组的心肌损伤较轻。②免疫组化染色法检测显示，A组Bcl-2的表达呈阳性，Bax的表达呈阴性；B组Bax的表达呈强阳性，Bcl-2的表达呈弱阳性；C组Bax的表达呈弱阳性，Bcl-2的表达介于A组与B组之间。③用电化学发光免疫法检测心肌酶学结果，CK-MB升高者3个组依次为B组>C组>A组，B组CK-MB的水平均明显高于C组与A组（P<0.01）。结论： BPS预处理对兔心肌I/R损伤早期具有拮抗作用。
- Abstract:
- AIM:To investigate the early antagonism of beraprost sodium (BPS) preconditioning on ischemic/reperfusion (I/R) injury in rabbit myocardium. METHODS: Thirty-six Japanese white rabbits were randomized into three groups: (A) sham operation group (thoracotomy and threading around left coronary artery without ligation), observation for 130 min, (B) I/R group (thoracotomy with left coronary artery ligation for 40 min and reperfusion for 90 min) and (C) BPS pretreatment group (BPS, 15 μg/kg, twice a day for 4 weeks, I/R within 3 h after the last administration). Group B and group C underwent the same time length of ischemia/reperfusion. After 90 min reperfusion, six rabbits were randomly selected for HE coloration and immunohistochemical detection of Bax and Bcl-2. After 4 h reperfusion, CK-MB in plasma was detected. RESULTS: HE stain showed that the myocardial structure was normal in group A, severely injured in group B and mildly in group C. Immunohistochemical stain showed that the expression of Bax was negative, whereas Bcl-2 was positive in group A. Bax was positive, whereas Bcl-2 was weakly positive in group B. Bax was weakly positive, whereas Bcl-2 was between that in group A and group B. There was an increase in CK. Group B>group C>group A. CONCLUSION: BPS may provide some early antagonism on ischemic/reperfusion injury in rabbit myocardium in vivo.
参考文献/References
[1]Miyamoto M,Tayama T,Yamaguchi S,et al.Improvement of walking disturbance by beraprost sodium in rat femoral artery occlusion models[J].Eur J Pharmacol,2006,545(2-3):173-176.
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[3]Ohta S,Nakamuta M,Fukushima M,et al.Beraprost sodium,a prostacyclin(PGI2)analogue, ameliorates concanavalin A-induced liver injury in mice[J].Liver International,2005,25(5):1061-1068.
[4]Yamada M,Sasaki R,Sato N,et al.Amelioration by beraprost sodium,aprostacyclin analogue,of established renal dysfunction in rat glomerulonephritis model[J].Eur J Pharmacol,2002,49(1-2):167-176.
[5]Yamada M,Sasaki R, Sato N,et al.Effects of beraprost sodium,an oral prostaglandin I2 analog, on hemostatic factors and inflammation in chronic peritoneal dialysis patients[J].Perit Dial Int,2009,29(2):178-181.
[6]Murry CE,Jennings RB,Reimer KA,et al.Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium[J].Circulation,1986,74(5):1124-1136.
[7]寇俊杰,王爱华,卫丹,等.IPC和缬沙坦在心肌缺血/再灌注损伤中的作用[J].心脏杂志,2009, 21(1):11-17.
备注/Memo
- 备注/Memo:
- 收稿日期：2010-09-21.通讯作者：王晓云，主任医师，主要从事缺血性心脏病研究Email：wxy@medmail.com.cn 作者简介：杨雪，硕士生Email：yangxue82324@163.com
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