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Title:: Effects of adenosine triphosphate postconditioning on myocardial ischemia/reperfusion injury in rabbits

作者:: 李妮妮¹; 廉哲勋²; 杨磊¹; 吕传君¹; 1.莱芜钢铁有限公司医院心内科，山东莱芜 271126；2.青岛大学医学院附属医院心内科

Author(s):: LI Ni-ni¹; LIAN Zhe-xun²; YANG Lei¹; LV Chuan-jun¹; Department of Cardiology, Laigang Hospital, Laiwu 271126, Shandong, China

关键词:: 心肌缺血/再灌注损伤; 缺血后处理; 白细胞介素-1β; 肿瘤坏死因子-α; 兔

Keywords:: myocardial ischemia/reperfusion injury; postconditioning; IL-1β; TNF-α; rabbit

分类号:: R542.2，Q524

DOI:: -

文献标识码:: A

摘要:: 目的:观察三磷酸腺苷(ATP)和腺苷A2a受体激动剂CGS-21680后处理对心肌缺血/再灌注损伤(MIRI)的影响，并探讨其作用的机制。方法：健康新西兰大白兔60只，随机分成5组（n=12）：即对照组、缺血/再灌注(I/R)组、缺血后处理(IPO)组、ATP组及CGS-21680组。建立兔心肌I/R模型，于再灌注末颈动脉取血，应用放射免疫测定法(RIA)测定血清中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)的含量；用TUNEL法检测心肌细胞的凋亡；实时荧光定量RT-PCR检测各组心肌组织中IL-1β mRNA和TNF-α mRNA的表达。结果： ①与I/R组比较， IPO组、ATP组和CGS21680组血清IL-1β、TNF-α的含量明显降低（P<0.01）；而3组组间相比较无显著差异。②IPO组、ATP组和CGS-21680组的细胞凋亡指数(AI)较I/R组明显降低，心肌组织损伤也显著减轻。同时，IPO组、ATP组和CGS-21680组IL-1β、TNF-α mRNA的表达明显低于I/R组；但3组间比较无显著差异。细胞凋亡和IL-1β、TNF-α mRNA的表达之间呈正相关（分别为r=0.91和r=0.93，P<0.05）。结论： ATP后处理可减轻MIRI，其作用机制可能与抑制炎症反应，减少细胞凋亡有关。

Abstract:: AIM:To explore the effects and mechanism of adenosine triphosphate and adenosine receptor agonist pharmacological postconditioning on myocardial ischemia/reperfusion (I/R) injury in rabbits. METHODS: Sixty New Zealand white male rabbits were randomly divided into five groups (n=12 each): control group, I/R group, IPO group, ATP group and CGS-21680 group. The model of myocardial ischemia/reperfusion was established and the concentrations of IL-1β and TNF-α were detected by radioimmunoassay at the end of 3 h reperfusion. TUNEL method was employed to detect apoptotic changes and real-time quantitative RT-PCR was used to detect the expression of IL-1β and TNF-α mRNA in myocardial tissue. RESULTS: Compared with those in I/R group, the concentrations of IL-1β and TNF-α markedly decreased in IPO group, ATP group and CGS-21680 group. However, the differences of three groups were not significant. Compared with those in I/R group, the apoptosis indexes significantly decreased in IPO group, ATP group and CGS-21680 group. The expressions of IL-1β and TNF-α mRNA decreased obviously in IPO group, ATP group and CGS-21680 group. A positive correlation was found between apoptosis and the expressions of IL-1β and TNF-α mRNA (r=0.91, r=0.93; P<0.05). CONCLUSION: ATP-postconditioning attenuates myocardial ischemia/reperfusion injury, possibly by inhibiting the effect of inflammation and decreasing myocardial apoptosis.

参考文献/References

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三磷酸腺苷后处理对心肌缺血/再灌注损伤的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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