我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

催产素诱导去分化脂肪细胞向心肌细胞方向分化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第6期
页码:
705-710,726
栏目:
基础研究
出版日期:
2011-12-25

文章信息/Info

Title:
Oxytocin induces cardiac differentiation of dedifferentiated FAT cells
作者:
杨 华; 陈连凤; 沈珠军
中国医学科学院北京协和医科大学北京协和医院心内科，北京 100730
Author(s):
YANG Hua;  CHEN Lian-feng;  SHEN Zhu-jun
Department of Cardiology, Peking Union Medical College Hospital, Beijing, 100730, China
关键词:
去分化脂肪细胞; 心肌分化; 催产素
Keywords:
dedifferentiated FAT;  cardiomyocyte differentiation;  oxytocin
分类号:
R984
DOI:
-
文献标识码:
A
摘要:
目的:检验催产素能否将去分化脂肪(DFAT)细胞诱导分化成为心肌样细胞。方法: 从脂肪中分离成熟的脂肪细胞,通过天花板培养使成熟脂肪细胞去分化为DFAT细胞。按照不同诱导的时间(1周、2周、3周)及低中高3个不同浓度的(1×10-8 mol/L、1×10-7 mol/L、1×10-6 mol/L)分组对DFAT进行诱导培养。通过免疫荧光检测法、RT-PCR及Western blot分别在基因水平和蛋白水平上检测心肌特异性标记的表达，以人类心肌组织作为阳性对照，未加诱导的DFAT细胞作为阴性对照。结果: 高浓度的催产素(1×10-6 mol/L)造成实验细胞大量死亡，无法进行后续试验。以1×10-8 mol/L及1×10-7 mol/L浓度的催产素诱导3周后，DFAT细胞在基因及蛋白水平上检测到心脏特异性标记GATA4、Nkx2.5及cTnT的表达，但未发现自主搏动现象。结论： 生理浓度和中等浓度的催产素，可以将 DFAT细胞诱导成为心肌样细胞。
Abstract:
AIM:To explore whether oxytocin (OT) could induce cardiomyogenic differentiation of DFAT in vitro. METHODS: Mature adipocytes were isolated from human adipose tissue and were dedifferentiated into DFAT via ceiling culture. Cultured DFAT cells were treated with different concentrations of OT (1×10-8 mol/L, 1×10-7 mol/L or 1×10-6 mol/L) for 1, 2 or 3 weeks. DFAT cells cultured without OT served as control. Cells were evaluated morphologically and immunocytochemically as well as by RT-PCR and Western blot. Human cardiomyocytes were used as positive control. RESULTS: Most DFAT cells treated with 1×10-6 mol/L OT died after 3 days. After treatment with oxytocin (1×10-8 mol/L, 1×10-7 mol/L) for 3 weeks, DFAT cells expressed cardiac markers including Nkx2.5, GATA4, and cardiac troponin T at both gene and protein levels. CONCLUSION: OT may induce cardiomyogenic differentiation of DFAT.

参考文献/References

[1]Matsumoto T,Kano K,Kondo D,et al.Mature adipocyte-derived dedifferentiated fat cells exhibit multilineage potential[J].J Cell Physio,2008,215(1):210-222.

[2]Gimpl G,Fahrenholz F.The oxytocin receptor system:structure,function,and regulation[J].Physiol Rev,2001,81(2):629-683.

[3]Sugihara H,Yonemitsu N,Miyabara S,et al.Primary cultures of unilocular fat cells:characteristics of growth in vitro and changes in differentiation properties[J]. Differentiation,1986,31(1):42-49.

[4]Jankowski M,Hajjar F,Kawas SA,et al.Rat heart:a site of oxytocin production and action[J].Proc Natl Acad Sci USA,1998,95(24):14558-14563.

[5]Gutkowska J,Jankowski M,Lambert C,et al.Oxytocin releases atrial natriuretic peptide by combining with oxytocin receptors in the heart[J].Proc Natl Acad Sci USA,1997,94(21):11704-11709.

[6]Gutkowska J,Jankowski M,Mukaddam-Daher S,et al.Oxytocin is a cardiovascular hormone[J].Braz J Med Biol Res,2000,33(6):625-633.

[7]Matsuura K,Nagai T,Nishigaki N,et al.Adult cardiac Sca-1-positive cells differentiate into beating cardiomyocytes[J].J Biol Chem,2004,279(12):11384-11391.

[8]Paquin J,Danalache BA,Jankowski M,et al.Oxytocin induces differentiation of P19 embryonic stem cells to cardiomyocytes[J].Proc Natl Acad Sci USA,2002,99(14):9550-9555.

[9]Hatami L,Valojerdi MR,Mowla SJ.Effects of oxytocin on cardiomyocyte differentiation from mouse embryonic stem cells[J].Int J Cardiol,2007,117(1):80-89.

备注/Memo

备注/Memo:
收稿日期：2011-03-01.通讯作者：沈珠军，教授，主要从事心血管疾病的研究 Email:zhujun66 shen@gmail.com 作者简介：杨华，硕士Email:yanghua198378@yahoo.com.cn
更新日期/Last Update: 2011-12-27