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|本期目录/Table of Contents|

雄激素缺乏对雄性大鼠血管内皮细胞的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第1期
页码:
28-30
栏目:
基础研究
出版日期:
2012-02-25

文章信息/Info

Title:
Effects of androgen deficiency on vascular endothelial cell in male rats
作者:
王艳妮; 谈世进
(上海交通大学附属第六人民医院老年病科，上海 200233)
Author(s):
WANG Yan-ni;  TAN Shi-jin
(Department of Geriatrics, Sixth People’s Hospital, Shanghai Jiaotong University, Shanghai 200233, China)
关键词:
雄激素; 血管内皮细胞; 一氧化氮; 内皮素-1
Keywords:
androgen;  vascular endothelial cell;  nitric oxide;  endothelin-1
分类号:
Q579.13
DOI:
-
文献标识码:
A
摘要:
目的:观察雄性大鼠去势后，雄激素缺乏对血管内皮细胞功能和结构的影响。方法： 雄性Wistar大鼠20只随机化分为单纯去势组（10只）和假手术组（10只）。8周后，用放射免疫法测定两组大鼠血浆睾酮的浓度，取胸主动脉HE染色后，观察血管内皮细胞的形态变化；采用化学比色法测定血浆一氧化氮（NO）的水平，用ELISA法检测血清内皮素-1（ET-1）的含量。结果： 与假手术组相比较，单纯去势组血浆睾酮的浓度及血浆NO的水平均显著降低，分别为［(2.47±0.53)μg/L vs.(0.28±0.07)μg/L和(33.44±8.50)μg/L vs.(21.61±10.51)μg/L，P<0.05］；而血浆ET-1的含量则明显升高［(3.84±0.16)μg/L vs.(4.41±0.34)μg/L，P<0.05］。单纯去势组血管平滑肌细胞的排列不整齐，管壁厚薄欠均匀，血管内皮细胞增生。结论： 雄性大鼠去势后雄激素缺乏可使大鼠血管内皮受损，释放NO减少以及ET-1增加。
Abstract:
AIM:To investigate the effects of androgen deficiency on vascular endothelial cell function and structure in castrated male rats. METHODS: Twenty male Wistar rats were randomly divided into simple castrated group (n=10) and sham-operated group (n=10). Blood testosterone concentration, nitric oxide (NO) and endothelin-1 (ET-1) were tested after 8 weeks. Pathological changes of thoracic aorta vascular endothelial cell were observed with HE stain. RESULTS: Compared with those in sham-operated group, testosterone concentration was significantly lower in simple castrated group (P<0.05) and blood plasma NO content in simple castrated group showed an obvious degradation ［(33.44±8.50) μg/L vs.(21.61±10.51) μg/L, P<0.05］. ET-1 levels of castrated group were significantly higher than the sham-operated group ［(4.41±0.34) μg/L vs.(3.84±0.16) μg/L, P<0.05］. Vascular smooth muscle cells in simple castrated group were of irregular arrangement and uneven wall thickness and vascular endothelial cells showed significant proliferation. CONCLUSIONS: Vascular endothelial cells in castrated male rats can be injured after androgen deficiency, with reduced NO and increased ET-1.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2009-07-05.通讯作者：谈世进，主任医师，主要从事老年心血管疾病的临床与基础研究 Email:sjxt@yahoo.com.cn 作者简介：王艳妮，硕士生 Email:wynnh@163.com
更新日期/Last Update: 2012-02-14