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|本期目录/Table of Contents|

色素上皮衍生因子对缺氧/复氧血管平滑肌细胞增殖及凋亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第2期
页码:
162-167
栏目:
基础研究
出版日期:
2012-04-25

文章信息/Info

Title:
Effects of pigment epithelium-derived factor on proliferation and apoptosis of vascular smooth muscle cells after hypoxia/reoxygen
作者:
刘振华; 顾春虎; 梁宏亮; 李子林; 杨 阳; 谢甲琦; 易定华
(第四军医大学西京医院心脏外科，陕西 西安 710032)
Author(s):
LIU Zhen-hua;  GU Chun-hu;  LIANG Hong-liang;  LI Zi-lin;  YANG Yang;  XIE Jia-qi;  YI Ding-hua
(Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military University, Xi’an 710032, Shaanxi, China)
关键词:
色素上皮衍生因子; 血管平滑肌细胞; 缺氧复氧; 细胞凋亡; 大鼠
Keywords:
pigment epithelial-derived factor;  vascular smooth muscle cells;  hypoxia/reoxygen;  cell apoptosis;  rats
分类号:
R392.11
DOI:
-
文献标识码:
A
摘要:
目的:观察重组人源性色素上皮衍生因子（Pigment epithelium-derived factor，PEDF）对缺氧/复氧(H/R)后平滑肌细胞（vascular smooth muscle cells，VSMCs）增殖和凋亡的影响。方法：以组织贴块法培养VSMCs后，随机分为3组，即对照组、H/R模型组及模型+PEDF组。模型+PEDF组又按PEDF的浓度(50、100、200及400 ng/ml)分为4组。用MTT比色法检测VSMCs增殖的变化；用Hoechst/PI双染色法和流式细胞仪法(FCM)检测VSMCs的凋亡；用Western blot法测定凋亡相关蛋白Bcl-2、Bax的表达。结果：①MTT比色法检测显示，缺氧、复氧可促进VSMCs增殖；PEDF能够抑制VSMCs增殖，以200 ng/ml的PEDF作用更显著。Hoechst/PI双染色法和FCM检测显示，经100、200 ng/ml的PEDF作用后，VSMCs的凋亡率较对照组和H/R组明显增高(P<0.01)。与对照组和H/R组比较，Western blot检测显示，100、200 ng/ml的PEDF能够下调Bcl-2蛋白表达并上调Bax蛋白表达(P<0.05，P<0.01)。结论：H/R后，VSMCs增殖增多，PEDF可抑制H/R后VSMCs增殖，其作用可能与Bcl-2、Bax通路促进VSMCs凋亡有关。
Abstract:
AIM:To explore the protective effect of recombinant human pigment epithelium-derived factor (PEDF) on vascular smooth muscle cell (VSMC) proliferation and apoptosis after hypoxia/reoxygen (H/R). METHODS: VSMCs were primarily cultured by tissue-sticking method and were randomly divided into control group, H/R model group and model+PEDF group. According to the concentration of PEDF, the model+PEDF group was subdivided into 50-, 100-, 200- and 400-ng/ml groups. VSMC proliferation was determined by MTT and cell apoptosis was detected by Hoechst/PI staining and flow cytometry. Bcl-2 and Bax protein expressions were tested by Western blot. RESULTS: MTT showed that H/R increased VSMC proliferation and PEDF inhibited VSMC proliferation. The inhibitory effect of 200 ng/ml PEDF was the most significant. After VSMCs were treated with 100 and 200 ng/ml PEDF, Hoechst/PI staining and flow cytometry demonstrated that the apoptosis rate of the cells significantly increased (P<0.01 vs. control group and H/R).Western blot demonstrated that 100 and 200 ng/ml PEDF downregulated Bcl-2 expression and upregulated Bax protein expression (P<0.05 or P<0.01 vs. control group and H/R group). CONCLUSION: H/R increases VSMC proliferation, whereas PEDF inhibits VSMC proliferation and promotes apoptosis after H/R, possibly through Bcl-2 and Bax pathways.

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备注/Memo

备注/Memo:
收稿日期：2011-10-25.基金项目：国家自然科学基金课题资助（81070183,81071276） 通讯作者：易定华，教授，主要从事心血管疾病的外科治疗研究 Email:yidh@fmmu.edu.cn 作者简介：刘振华，硕士生 Email:jackly0825@21cn.com
更新日期/Last Update: 2012-04-01