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Title:: Effect of probucol on advanced glycosylation end product-induced dysfunction in cardiac microvascular endothelial cells

作者:: 成永霞¹; 刘贵波²; 于建渤¹; 郭素芬¹; 颜彬¹ ; 李志强¹; 杨向红³; (牡丹江医学院：1.病理学教研室，2.解剖学教研室，黑龙江牡丹江157011；3.中国医科大学附属盛京医院病理科，沈阳 100011)

Author(s):: CHENG Yong-xia¹; LIU Gui-bo²; YU Jian-bo¹; GUO Su-fen¹; YAN Bin¹; Li Zhi-qiang¹; YANG Xiang-hong³; (1.Department of Pathology, 2.Department of Anatomy, Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang, China; 3.Department of Pathology, Affiliated Shengjing Hospital, China Medical University, Shenyang 100011, China)

关键词:: 心脏微血管内皮细胞; 糖基化终末产物; 普罗布考; 诱导型一氧化氮合酶

Keywords:: cardiac microvascular endothelial cells; advanced glycosylation end products; probucol; iNOS

分类号:: R36;R972

DOI:: -

文献标识码:: A

摘要:: 目的:观察普罗布考（probucol）对糖基化终末产物（AGEs）作用后的心脏微血管内皮细胞iNOS表达的影响。方法：原代培养心脏微血管内皮细胞，AGEs（100 mg/L）体外模拟高糖环境，在probucol(5 μmol/L,10 μmol/L,20 μmol/L) 作用后，检测ROS、NO和iNOS变化情况。结果：与AGEs组比较，probucol组中ROS蛋白表达降低，NO生成增加，而 iNOS蛋白表达降低，且显示有浓度依赖性。结论： probucol可能通过对抗氧化应激的途径，缓解AGEs引发的心脏微血管内皮功能障碍。

Abstract:: AIM:To observe the effect of probucol on the iNOS expression induced by advanced glycosylation end products (AGEs) in cardiac microvascular endothelial cells. METHODS: Primary cardiac microvascular endothelial cells were cultured in vitro. After treatment with AGEs (100 mg/L) and probucol (5 μmol/L, 10 μmol/L, 20 μmol/L), NO, iNOS and ROS protein expressions in all groups were detected and compared between groups. RESULTS: Compared with AGEs group, NO and iNOS protein expression increased and ROS protein expression decreased in groups treated with probucol. CONCLUSION: Probucol is able to partially recover AGE-induced dysfunction in cardiac microvascular endothelial cells.

参考文献/References

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普罗布考对糖基化终末产物引发心脏微血管内皮功能紊乱的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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