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回转器模拟失重对静脉内皮细胞形态、增殖和周期的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第4期
页码:
426-429
栏目:
基础研究
出版日期:
2012-08-25

文章信息/Info

Title:
Effects of simulated microgravity on cell morphology, cell proliferation and cell cycle in human umbilical vein endothelial cells
作者:
王永春; 王 攀; 张 舒; 王 冰; 耿 捷; 杜挺媛; 孙喜庆
(第四军医大学航空航天医学系航空航天生物动力学教研室、航空航天医学教育部重点实验室，陕西 西安 710032)
Author(s):
WANG Yong-chun;  WANG Pan;  ZHANG Shu;  WANG Bing;  GENG Jie;  DU Ting-yuan;  SUN Xi-qing
(Department of Biodynamics, School of Aerospace Medicine, Fourth Military Medical University & Key Laboratory of Aerospace Medicine, Education Ministry of China, Xi’an 710032, Shaanxi, China)
关键词:
模拟失重; 人脐静脉内皮细胞; 细胞形态; 细胞增殖; 细胞周期
Keywords:
simulated microgravity;  human umbilical vein endothelial cells;  cell morphology;  cell proliferation;  cell cycle
分类号:
R852.22
DOI:
-
文献标识码:
A
摘要:
目的:探讨回转器模拟失重对人脐静脉内皮细胞(HUVECs)形态、增殖和周期的影响。方法： 以HUVECs为研究对象，采用回转器模拟失重，在倒置显微镜下观察模拟失重后细胞形态的变化，用细胞计数法测定细胞的增殖情况，用流式细胞仪(FCM)测定细胞周期。结果： 模拟失重后，HUVECs多呈圆形，可见细胞重叠或聚集生长。回转器模拟失重后12 h、24 h和36 h，细胞周期明显阻滞在G0+G1期（P<0.05），而细胞计数则无明显改变。结论： 模拟失重可使HUVECs的形态发生一定改变，使HUVECs的细胞周期阻滞。
Abstract:
AIM:To investigate the effects of simulated microgravity on cell morphology, cell proliferation and cell cycle in human umbilical vein endothelial cells (HUVECs). METHODS: Clinostat was used to simulate microgravity conditions and cell morphological changes were measured using an inverted microscope. Cell proliferation was measured by cytometry and cell cycle alterations were examined by flow cytometer. RESULTS: Compared with those in the control group, HUVECs became round and grew in an overlapping manner in the clinorotation group. Cell numbers in clinorotation groups did not change significantly. Cell numbers in clinorotation group in G0+G1 phase at 12 h, 24 h and 36 h time-points increased significantly compared with those in controls (P<0.05). CONCLUSION: Simulated microgravity induces changes of cell morphology and inhibition of cell cycle in HUVECs.

参考文献/References

[1]Hughes-Fulford M,Lewis ML.Effect of microgravity on osteoblast growth activation[J].Exp Cell Res,1996,224(1):103-109.

[2]Wang YC,Zhang S,Du TY,et al.Clinorotation upregulates inducible nitric oxide synthase by inhibiting AP-1 activation in human umbilical vein endothelial cells[J].J Cell Biochem,2009,107(2):357-363.

[3]Carlsson SI,Bertilaccio MT,Ballabio E.Endothelial stress by gravitational unloading: effects on cell growth and cytoskeletal organization[J].Biochim Biophys Acta,2003,1642(3):173-179.

[4]Morbidelli L,Monici M,Marziliano N,et al.Simulated hypogravity impairs the angiogenic response of endothelium by up-regulating apoptotic signals[J]. Biochem Biophys Res Commun,2005,334(2):491-499.

[5]Infanger M,Ulbrich C,Baatout S,et al.Modeled gravitational unloading induced downregulation of endothelin-1 in human endothelial cells[J].J Cell Biochem,2007,101(6):1439-1455.

[6]Slentz DH,Truskey GA,Kraus WE.Effects of chronic exposure to simulated microgravity on skeletal muscle cell proliferation and differentiation[J].In Vitro Cell Dev Biol Anim,2001,37(3):148-156.

[7]Harris SA,Zhang M,Kidder LS.Effects of orbital spaceflight on human osteoblastic cell physiology and gene expression[J].Bone,2000,26(4):325-331.

[8]Chiu B,Wan JZ,Abley D,et al.Induction of vascular endothelial phenotype and cellular proliferation from human cord blood stem cells cultured in simulated microgravity[J].Acta Astronaut,2005,56(9-12):918-922.

[9]Cotrupi S,Ranzani D, Maier JA.Impact of modeled microgravity on microvascular endothelial cells[J].Biochim Biophys Acta, 2005,1746(2):163-168.

[10]Rita C,Angela C,Grazia G.Exposure to modeled microgravity induces metabolic idleness in malignant human MCF-7 and normal murine VSMC cells[J].FEBS Letters,2006, 580(10):2465-2470.

[11]张晓铀,汪恭质,丁 柏,等.模拟失重对成骨样细胞细胞周期变化的影响[J].中华航空航天医学杂志,2000,11(1):43-45.

[12]朱 鸣,吴本俨,聂捷琳,等.回转器模拟失重对人胃癌SGC-7901细胞形态和生物学特征的影响[J].中华航空航天医学杂志,2005,16(4):262-266.

[13]高 燕,林莉萍,丁 健.细胞周期调控的研究进展[J]生命科学,2005,17(4):318-322.

备注/Memo

备注/Memo:
收稿日期：2012-02-13.基金项目：国家自然科学基金项目资助(30900279，81171872)；第四军医大学青年英才项目资助 通讯作者：孙喜庆，教授， 主要从事航空航天医学研究Email：sunxiqing@tom.com 作者简介：王永春，讲师，博士Email：wangych0526@hotmail.com
更新日期/Last Update: 2012-07-20