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|本期目录/Table of Contents|

重组人脑钠尿肽后适应对兔急性缺血/再灌注心肌的保护作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第5期
页码:
569-572
栏目:
基础研究
出版日期:
2012-10-25

文章信息/Info

Title:
Protective effect of rhBNP postconditioning against acute myocardial ischemia/reperfusion injury in rabbits
作者:
田学峰1; 王晓云2; 梁 明1; 王嵬民1; 车 琳1 ; 孙 艳1
(1.黑龙江省医院心内科，黑龙江 哈尔滨 150036；
2.哈尔滨医科大学附属第四医院心内科，黑龙江 哈尔滨 150001)
Author(s):
TIAN Xue-feng1;  WANG Xiao-yun2;  LIANG Ming1;  WANG Wei-min1;  CHE Lin1;  SUN Yan1
(1.Deparment of Cardiology, Heilongjiang Provincial Hospital, Harbin 150036, Heilongjiang, China; 2.Department of Cardiology, Fourth Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China)
关键词:
心肌梗死; 缺血/再灌注; 脑钠肽后适应; 兔
Keywords:
myocardial ischemia reperfusion injury;  BNP;  apoptosis Bcl-2;  Bas
分类号:
R541.1
DOI:
-
文献标识码:
A
摘要:
目的:探讨重组人脑利钠肽(rhBNP)后适应对兔急性缺血/再灌注（I/R）心肌的保护作用及其机制。 方法： 将36只健康日本大耳白兔随机分为3组（每组12只）。即假手术组、I/R组（缺血40 min后再灌注180 min）及BNP+I/R组（I/R前5 min以0.01 μg/kg静脉给予BNP）。假手术组开胸于左室支下穿线但不结扎，观察180 min结束；I/R组及BNP+I/R组：缺血40 min，分别再灌注180 min后处死。进行心肌酶学CK-MB含量检测，观察心肌HE染色后病理形态变化及BCI-2、Bax表达的检测。结果： 与假手术组相比，I/R组和BNP+I/R组CK-MB的水平均显著增高（P<0.01)。与I/R组相比，BNP+I/R组明显减少这些心肌酶的升高（P<0.01)。与假手术组相比，I/R组及BNP+I/R组均可见到凋亡细胞，但BNP+I/R组凋亡细胞明显减少。与假手术组相比，I/R组Bax的表达增加，Bcl-2的表达降低。与I/R组相比，BNP+I/R组 Bax的表达明显降低，而Bcl-2的表达明显升高。结论： 重组人脑钠尿肽可减少梗死后心肌的损伤；可以增加梗死后心肌细胞抑制凋亡基因（Bcl-2）的表达，从而减少心肌细胞的凋亡。
Abstract:
AIM:To study the protective effect of rhBNP postconditioning on acute myocardial ischemia/reperfusion injury in rabbits and its mechanism. METHODS: Thirty-six healthy rabbits were randomly divided into three groups with 12 rabbits in each group: sham-operated group, ischemia-reperfusion group and BNP postconditioning group. Blood vessels were not occluded in sham-operated group, a 40-min occlusion of the left coronary vessel was followed by a 180-min reperfusion of physiological saline in ischemia-reperfusion group and BNP was injected 5 min before and throughout the whole reperfusion in the BNP postconditioning group. Serum CK-MB was detected and recorded. Expressions of Bcl-2 and Bas were examined. RESULTS: Levels of CK-MB markedly increased in ischemia reperfusion group (P<0.01) compared with those in sham-operated group. The elevation of CK-MB obviously reduced in BNP postconditioning group. Myocardial cell apoptosis significantly reduced in BNP postconditioning group compared with that in I/R group. The expression of Bax increased and the expression of Bcl-2 decreased, which were contrary to those in BNP postconditioning group. CONCLUSION: Biochemical examinations show that BNP reduces myocardial infarction injury and increases the expression of Bcl-2, thus lowering myocardial apoptosis.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2012-06-09.
基金项目：黑龙江省科技厅青年基金项目资助（QC2011C123）
作者简介：田学峰，主治医师，硕士Email:xuelian632@163.com
更新日期/Last Update: 2012-11-16