«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2012年第6期

页码:

673-676,685

栏目:

基础研究

出版日期:

2012-12-25

文章信息/Info

Title:

Effects of simulated microgravity on rat cardiomyocyte apoptosis

作者:

高 原; 孙喜庆; 杜挺媛; 石 菲; 张 舒;  王 冰; 王永春

(第四军医大学航空航天医学系航空航天生物动力学教研室、航空航天医学教育部重点实验室，陕西 西安 710032)

Author(s):

GAO Yuan;  SUN Xi-qing;  DU Ting-yuan;  SHI Fei;  ZHANG Shu;  WANG Bing;  WANG Yong-chun

(Department of Aerospace Biodynamics, School of Aerospace Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)

关键词:

模拟失重; 心肌细胞; 大鼠; 细胞凋亡

Keywords:

simulated microgravity;  cardiomyocytes;  rats;  apoptosis

分类号:

R851.22

DOI:

-

文献标识码:

A

摘要:

目的:探讨模拟失重对体外培养的大鼠心肌细胞凋亡的影响。方法： 以原代培养的乳鼠心肌细胞为研究对象，以细胞回转器模拟失重效应，通过流式细胞检测技术观察模拟失重96 h对心肌细胞凋亡率的影响，进一步通过RT-PCR的方法，检测模拟失重96 h后，心肌细胞凋亡相关基因p53、bcl-2和caspase-3 mRNA表达的影响。结果： 模拟失重96 h可显著增高心肌细胞的凋亡率(P<0.05)，与对照组相比，回转器模拟失重可显著增高促凋亡基因p53、caspase-3 mRNA的表达(P<0.05)，而抑凋亡基因bcl-2 mRNA则表达显著减低(P<0.05)。结论： 回转器模拟失重96 h致心肌细胞凋亡表达增高，其可能机制是凋亡相关基因表达失衡有关。

Abstract:

AIM:To investigate the effects of simulated microgravity on apoptosis of neonatal primary rat cardiomyocytes. METHODS: Clinostat was used to simulate microgravity condition. Apoptosis rates were examined by flow cytometry, and mRNA expression of p53, bcl-2 and caspase-3 were measured by RT-PCR methods. RESULTS: Flow cytometry results showed that the rates of apoptotic cardiomyocytes were higher than those in control group (P<0.05). Compared with those in control group, mRNA expression of p53 and caspase-3 increased significantly (P<0.05), whereas bcl-2 mRNA expression decreased significantly (P<0.05). CONCLUSION: Simulated microgravity induces apoptosis of neonatal primary rat cardiomyocytes.

参考文献/References

[1]孙喜庆,姜世忠.空间医学与生物学研究[M].西安:第四军医大学出版社,2010.
[2]Blomqvist CG. Regulation of the systemic circulation at microgravity and during readaptation to 1G[J].Med Sci Sports Exerc,1996,28(10 Suppl):S9-S13.
[3]Sun XQ,Yao YJ,Wu XY,et al.Effect of lower body negative pressure against orthostatic intolerance induced by 21 days head-down tilt bed rest［J］. Aviat Space Environ Med,2002,73(4):335-340.
[4]余志斌,张立藩.心肌功能对模拟失重状态的适应[J].航天医学与医学工程,1997,10(3): 206-220.
[5]Goldstein MA.Cardiac morphology after conditions of microgravity during COSMOS 2044[J].J Appl Physiol,1992,73(2):94-100.
[6]李莹辉,丁柏,汪恭质,等.重力因素对体外培养心肌细胞的影响作用[J].中华航空航天医学杂志,1999,10(2):100.
[7]王俊锋,郝从均,刘长庭,等.N-乙酰半胱氨酸对尾悬吊模拟失重大鼠肺组织细胞凋亡的影响[J].军医进修学院学报,2007,28(4):254-256.
[8]朱鸣，吴本俨,聂捷琳,等.回转器模拟失重对SGC-7901和HFE-145细胞凋亡的影响[J].世界华人消化杂志,2009,17(24):2491-249.
[9]Kang CY,Zou L,Yuan M,et al.Impact of simulated microgravity on microvascular endothelial cell apoptosis[J].Eur J Appl Physio,2011,111(9):2131-2138.
[10]Chang H,Zhang L,Xu PT,et al.Nuclear translocation of calpain-2 regulates propensity toward apoptosis in cardiomyocytes of tail-suspended rats[J].J Cell Biochem,2011,112(2):571-580.
[11]张文辉,亓鹏,杨芬,等.模拟失重对大鼠心肌细胞凋亡影响的实验研究[J].航天医学与医学工程,2009,22(4):252-254.
[12]张丽娜,吴星恒.心肌细胞凋亡与PI3K/Akt信号途径表达及其调控机制的研究[J].南昌大学学报(医学版),2010,50(5):119-121.

备注/Memo

备注/Memo:

收稿日期：2012-06-27.基金项目：国家自然科学基金项目资助(30900279，81171872) 通讯作者：王永春，讲师，主要从事航空航天医学与生物学研究 Email:wangych0526@hotmail.com 作者简介：高原，讲师 Email:gaoyuan1109@hotmail.com

常用功能

更新日期/Last Update: 2012-12-30