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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2012年第6期

页码:

681-685

栏目:

基础研究

出版日期:

2012-12-25

文章信息/Info

Title:

Effect of qidan tongmai tablet on myocardial fibrosis in postmyocardial infarction rats

作者:

衣 慧¹; 历 志²; 王 文¹; 司静文¹; 马 静¹; 王宗仁¹; 李军昌¹

(第四军医大学第一附属医院：1.中医科，2.心外科，陕西 西安 710032)

Author(s):

YI Hui¹;  LI Zhi²;  WANG Wen¹;  SI Jing-wen¹;  MA Jing¹;  WANG Zong-ren¹;  LI Jun-chang¹

(1.Department of Traditional Chinese Medicine, 2.Department of Cardiac Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)

关键词:

芪丹通脉片; 心肌梗死; 心肌纤维化; 重构; 大鼠

Keywords:

qidan tongmai tablet;  myocardial infarction;  myocardial fibrosis;  reconfiguration;  rat

分类号:

R542.22

DOI:

-

文献标识码:

A

摘要:

目的:观察益气活血复方芪丹通脉片（qidan tongmai tablet，QDTMT）对大鼠心肌梗死(MI)后心功能及左心室非梗死区心肌纤维化的影响。方法： 以结扎SD大鼠左冠脉前降支法建立MI模型，随机分为假手术(Sham)组、MI组、MI+QDTMT小剂量(MI-QDTMTL)组和MI+QDTMT大剂量(MI-QDTMTH)组。术后24 h各组均用生理盐水配制成等体积药液灌胃4周，4周后以多普勒超声评价心脏功能；测定心室的质量/体质量(HW/BW)；以MASSON染色检测非梗死区胶原的含量；用RT-PCR法检测非梗死区转化生长因子-β1（TGF-β1）、Ⅰ型胶原蛋白（Collagen type 1，Col1）及Ⅲ型胶原蛋白（Collagen type 3，Col3）mRNA的表达水平。采用大鼠羟脯氨酸（HYP）的ELASA试剂盒检测非梗死区中HYP的含量。结果： ①术后4周心功能：与MI组比较，MI-QDTMTL组和MI-QDTMTH组左室舒张末期内径(LVEDD)和HW/BW均降低，而射血分数(EF)升高（P<0.01或P<0.05）；②非梗死区心肌胶原蛋白的含量: MI-QDTMTL组胶原和MI-QDTMTH组胶原含量均低于MI组（P<0.01）；MI-QDTMTH组胶原的含量明显低于MI-QDTMTL组(P<0.01)；③非梗死区TGF-β1、Col1、Col3mRNA的表达：与MI组比较，MI-QDTMTL组、MI-QDTMTH组的TGF-β1 Col1、Col3 mRNA均显著降低(P<0.01或P<0.05)；MI-QDTMTH组TGF-β1、Col1、Col3 mRNA的表达显著低于MI-QDTMTL组(P<0.05)；④非梗死区HYP的含量： MI-QDTMTL组与MI-QDTMTH组HYP的含量低于MI组(P<0.05，P<0.01)； MI-QDTMTH组HYP的含量低于MI-QDTMTL组(P<0.05)。结论： QDTMT通过下调MI交界区TGF-β1、Col1、Col3 mRNA的表达及HYP产生，进而抑制MI后非梗死区反应性胶原的过度沉积，且高剂量组比低剂量组的疗效更好，为防治MI后非梗死区心肌纤维化重构，改善心脏功能提供了新的治疗思路。

Abstract:

AIM:To investigate the effect of qidan tongmai tablet (QDTMT) on cardiac function and myocardial fibrosis in rats after myocardial infarction (MI). METHODS: Sprague Dawley (SD) rats were subjected to MI by ligating the left anterior descending coronary and randomly divided into sham group, MI group, low-dose QDTMT group (MI-QDTMTL, 1.0 g/kg) and high-dose QDTMT group (MI-QDTMTH, 2.0 g/kg). After 24 h of ligation, 15 ml/kg saline for sham and MI groups and 1.0 g/kg or 2.0 g/kg QDTMT for MI-QDTMTL or MI-QDTMTH groups were administered twice daily. Cardiac contractile function of left ventricular end-diastolic pressure (LVEDP) and ejection factor (EF) were measured in vivo. Rats were sacrificed after 4 weeks. Collagen content in the noninfarcted area was detected by Masson’s trichrome stain. Transforming growth factor beta-1 (TGF-β1), collagen type 1 and collagen type 3 mRNA expressions were examined by RT-PCR in the noninfarcted area. The hydroxyproline content of the noninfarcted tissue was measured by hydroxyproline (HYP) ELISA kit for rats. RESULTS: After 4 weeks, rat cardiac function of LVEDD and EF and the heart weight/body weight (HW/BW) index in QDTMTH group and QDTMTL group were significantly better than those in MI group (P<0.01; P<0.05), but poorer than those in the sham group (P<0.01). Myocardial collagen content in noninfarcted area was significantly inhibited in MI-QDTMTL group and MI-QDTMTH group (P<0.01). mRNA expression levels of TGF-β1, Col1 and Col3 in noninfarcted area were significantly inhibited in MI-QDTMTL group and MI-QDTMTH group (P<0.01). Hydroxyproline content of the noninfarcted tissue was significantly inhibited in MI-QDTMTL group and MI-QDTMTH group (P<0.05). CONCLUSION: In post-MI myocardial fibrosis, QDTMT improves cardiac function and reduces myocardial fibrosis by downregulating TGF-β1, Col1and Col3 expressions and decreasing the production of hydroxyproline.

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备注/Memo

备注/Memo:

收稿日期：2012-05-25.基金项目：国家自然科学基金项目资助（81072972） 通讯作者：李军昌，副教授，主要从事中西医结合心血管病的研究 Email:lijunch@fmmu.edu.cn 作者简介：衣慧，硕士生 Email:wjcyh99@126.com

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更新日期/Last Update: 2012-12-30