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|本期目录/Table of Contents|

低频脉冲磁场调节NO/ONOO-对缺氧/复氧条件下心肌细胞的保护作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2013年第2期
页码:
134-139
栏目:
基础研究
出版日期:
2013-04-25

文章信息/Info

Title:
Low-frequency pulsed magnetic fields protect cardiomyocytes from hypoxia/reoxygenation injury by affecting the “NO/ONOO- balance”
作者:
张正勋; 易 甫; 范伟伟; 张荣庆; 王东娟; 李秀娟; 曹 丰
(第四军医大学西京医院心脏内科，陕西 西安 710032)
Author(s):
ZHANG Zheng-xun;  YI Fu;  FAN Wei-wei;  ZHANG Rong-qing;  WANG Dong-juan;  LI Xiu-juan;  CAO Feng
(Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
低频脉冲磁场; 心肌细胞; 缺氧/复氧; NO/ONOO-平衡
Keywords:
Low-frequency pulsed magnetic fields;  cardiomyocyte;  hypoxia/reoxygenation;  NO/ONOO- balance
分类号:
R541.83；R454.1
DOI:
-
文献标识码:
A
摘要:
目的:观察低频脉冲磁场（LF-PMFs）对缺氧/复氧（H/R）条件下乳鼠心肌细胞增殖与凋亡的影响，并探讨一氧化氮/过氧亚硝基阴离子（NO/ONOO-）平衡在LF-PMFs保护心肌细胞的作用。方法： 采用酶消化法分离SD乳鼠心肌细胞，随机分为对照组、H/R组和磁场组。用TUNEL法检测心肌细胞凋亡程度，MTT比色法检测心肌细胞增殖活性，以观察LF-PMFs对损伤心肌细胞的效果，用WST-1 法检测超氧阴离子，Griess法测定NO和ONOO-。结果： LF-PMFs磁场可减少H/R诱导的心肌细胞凋亡，并促进心肌细胞的存活，以15 Hz，4.5 mT LF-PMFs磁场的作用效果最为显著。同时LF-PMFs可减少H/R条件下乳鼠心肌细胞超氧阴离子的生成，促进NO生成，提高NO/ONOO-比值。结论： LF-PMFs可通过抗氧化及调节NO/ONOO-平衡减轻H/R导致的心肌细胞损伤。
Abstract:
AIM:To investigate the effect of low-frequency pulsed magnetic fields (LF-PMFs) on proliferation and apoptosis of neonatal cardiomyocytes after hypoxia/reoxygenation (H/R) injury and the mechanism of NO/ONOO- balance involved. METHODS: Cultured neonatal cardiomyocytes were randomized into three groups: control group, H/R group, and LF-PMFs+H/R group. Viability and apoptosis of cardiomyocytes were assessed by MTT and TUNEL and levels of superoxide anion and NO/ONOO- were tested by Superoxide Assay Kit and Griess Assay, respectively. RESULTS: LF-PMFs promoted proliferation and inhibited apoptosis of neonatal cardiomyocytes following H/R treatment. Pre-/pro-treatment with LF-PMFs significantly downregulated the level of superoxide anion and ONOO- and upregulated NO expression of cardiomyocytes in vitro. LF-PMFs with 15 Hz, 4.5 mT yielded the most beneficial effect compared with magnetic fields at other levels. CONCLUSIONS: LF-PMFs exert protective effects on cardiomyocytes from H/R injury via balancing NO/ONOO-.

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备注/Memo

备注/Memo:
收稿日期：2012-11-12.基金项目：国家自然科学基金项目资助（81000686） 通讯作者：曹丰，教授，主要从事冠心病与干细胞分子影像研究 Email:wind8828@gmail.com 作者简介：张正勋，硕士生 Email:x728823@foxmail.com
更新日期/Last Update: 2013-04-28