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|本期目录/Table of Contents|

电压门控钾通道在慢性缺氧性肺动脉高压发展中的作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2013年第4期
页码:
385-388
栏目:
基础研究
出版日期:
2013-07-25

文章信息/Info

Title:
Roles of voltagegated potassium channels in progression of chronic hypoxic pulmonary hypertension
作者:
柯琴梅1; 吴 霁1; 杜以梅2; 田 莉3; 李 伟1; 朱元州4
(华中科技大学附属协和医院：1.老年病科，2.心内科，3.儿科；4.武汉科技大学附属天佑医院重症医学科，湖北 武汉 430022)
Author(s):
KE Qin mei1;  WU Ji1;  DU Yi mei2;  TIAN Li3;  LI Wei1;  ZHU Yuan zhou4
(1.Department of Geriatrics, 2.Department of Cardiology, 3.Department of Pediatrics, Union Hospital, Huazhong Science and Technology University; 4.Department of Severe Medicine, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan 430022, Hubei, China)
关键词:
钾通道; 慢性缺氧性肺动脉高压; 缺氧性肺血管收缩; 大鼠
Keywords:
potassium channel;  pulmonary hypertension;  hypoxia pulmonary vasoconstriction;  rat
分类号:
R543.2
DOI:
-
文献标识码:
A
摘要:
目的:探究慢性缺氧对肺动脉平滑肌细胞电压门控钾通道（Kv）的影响及其在慢性缺氧性肺动脉高压发生发展中的作用。方法:50只雄性SD大鼠随机分为常氧对照组（10只）和慢性缺氧5 d、10 d、20 d及30 d组（各10只）。慢性缺氧组大鼠每天在低氧仓中予以缺氧8 h，分别取缺氧5 d、10 d、20 d及30 d的大鼠进行实验。测量平均肺动脉压（mPAP）并应用全细胞膜片钳技术记录肺动脉平滑肌细胞电压门控钾通道电流（IK）。结果:慢性缺氧显著减低大鼠肺动脉平滑肌细胞的IK峰值及IV曲线漂移。慢性缺氧5 d组肺动脉平滑肌细胞的IK密度及IV曲线与常氧组均没有显著差异；而慢性缺氧10 d组肺动脉平滑肌细胞的IK密度及IV曲线与常氧组均有显著差异(P< 0.05)，随着缺氧时间的延长，IK密度的峰值进一步降低。与常氧组相比较，慢性缺氧10 d组大鼠的mPAP明显增加(P<0.05)，随着缺氧时间的增加，mPAP进一步增加；mPAP的增加与IK密度的下降呈负相关(r=-0.89769，P<0.01)。结论:慢性缺氧在引发肺动脉高压的过程中伴随有肺动脉平滑肌细胞Kv通道的活性降低，提示Kv参与了肺动脉高压的发生发展。
Abstract:
AIM:To observe the influence of chronic hypoxia on the activity of voltagegated potassium channel in pulmonary artery smooth muscle cells of rats and its roles in the progression of chronic pulmonary hypertension. METHODS: Fifty male Sprague Dawley rats were randomly allocated into normoxic group (n=10) and chronic hypoxic groups. The chronic hypoxic groups were randomly allocated into four subgroups (n=10, respectively) according to the chronic hypoxic periods. The chronic hypoxic subgroups were kept in a hypoxic environmental chamber 8 h/day for 5, 10, 20, and 30 days, respectively, while the normoxic group was kept in room air. The mean pulmonary arterial pressure (mPAP) and the current of voltagegated potassium channel (IK) in pulmonary artery smooth muscle cells were measured using conventional whole cell patchclamp technique. RESULTS: No significant difference was observed in the density of IK (at +60 mV) and the IV relationship between normoxic group and the group exposed to chronic hypoxia for 5 days (P>005), whereas significant differences were found between the normoxic group and the group exposed to chronic hypoxia for 10 days (P<005). With the prolonged exposure to hypoxia, the peak density of IK decreased gradually at +60 mV. In normoxic rats, the mPAP was significantly increased after exposure to chronic hypoxia for 10 days (P<005) and prolonged exposure to hypoxia further increased the mPAP. The mPAP was negatively correlated with the density of IK (r=-089769, P<001). CONCLUSION: Exposure to chronic hypoxia may cause decreased activity of voltagegated potassium channel, which leads to hypoxic pulmonary vasoconstriction. This mechanism plays an important role in the progression of chronic pulmonary hypertension.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2012-12-04.基金项目：湖北省自然科学基金项目资助(2004ABA191) 通讯作者：杜以梅，副教授，主要从事心血管电生理研究 Email:yimeidu@hotmail.com 作者简介：柯琴梅，副教授，博士 Email:qmke229@yahoo.com.cn
更新日期/Last Update: 2013-07-29