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胺碘酮对兔心肌缺血/再灌注心肌细胞凋亡及Bcl-2、Bax蛋白表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2013年第4期
页码:
400-403407
栏目:
基础研究
出版日期:
2013-07-25

文章信息/Info

Title:
Effects of amiodarone on cardiomyocyte apoptosis and expressions of Bcl2 and Bax protein after ischemia/reperfusion injury in rabbits
作者:
信栓力1; 2; 杨 华2; 常 超1; 刘丽军1; 孟利民1
(1.邯郸市第一医院心内科，河北 邯郸 056002；2.河北医科大学研究生院，河北 石家庄 050017)
Author(s):
XIN Shuanli1; 2;  YANGHua-2;  CHANGChao1-;  LIU Lijun1;  MENG Limin1
(1.Department of Cardiology, First Hospital of Handan, Handan 056002, Hebei, China; 2.Graduate School, Hebei Medical University, Shijiazhuang 050017, Hebei, China)
关键词:
胺碘酮; 缺血/再灌注; 细胞凋亡; Bc-2蛋白; Bax蛋白; 兔
Keywords:
amiodarone;  ischemia/reperfusion;  no reflow;  Bcl2 protein;  Bax protein;  rat
分类号:
R972.2
DOI:
-
文献标识码:
A
摘要:
目的:探讨胺碘酮(AM)对兔心肌缺血/再灌注损伤(I/RI)心肌细胞凋亡及凋亡相关蛋白Bcl-2、Bax表达的影响。方法:将60只新西兰大白兔随机分为假手术（Sham）组、缺血/再灌注（I/R）组和AM组，每组20只。Sham组开胸只穿线不结扎血管，而其余两组开胸并结扎兔左冠状动脉回旋支制备I/R无复流模型。应用硫磺素S染色评估心肌无复流面积。经膜联蛋白V-异硫氰酸荧光素（annexin V-FITC）和碘化丙啶(PI)双标记后，应用流式细胞术检测心肌细胞并计算凋亡率。采用免疫组化染色法检测心肌细胞中Bcl-2、Bax蛋白的表达。结果：①在缺血范围差异无统计学意义的条件下，AM组无复流范围显著小于I/R组(t=4.483，P<0.01)。②与Sham组相比，I/R组和AM组细胞的凋亡率明显增加(F=315.25，P<0.01)。与I/R组相比，AM组细胞的凋亡率明显降低(F=315.25，P<0.01)。③与Sham组相比，I/R组和AM组Bcl-2、Bax蛋白的表达明显增加分别为(F=29.01，P<0.01和F=214.45，P<0.01)。与I/R组相比，AM组Bcl-2蛋白的表达明显增加(F=29.01，P<0.01)；Bax蛋白的表达明显减少(F=214.45，P<0.01)。与I/R组相比，AM组Bcl-2/Bax比值明显增加(F=97.61，P<0.01)。结论:AM可能通过具有抗I/R心肌细胞凋亡从而减轻无复流范围，至于抗凋亡和减轻无复流的具体机制有待进一步明确。
Abstract:
AIM:To investigate the effect of amiodarone on cardiomyocyte apoptosis and expressions of Bcl2 and Bax protein after ischemia/reperfusion (I/R) injury in rabbits. METHODS: Sixty mature rabbits were randomized into sham group, I/R group and amiodarone (AM) group with 20 rabbits in each group. I/R group and AM group were subjected to 90 min of occlusion in the left circumflex artery followed by 2 h of reperfusion to establish the myocardial I/R injury model. After 120 min reperfusion, the noreflow (NR) areas were determined by thioflavin S. Apoptosis rate was determined with flow cytometer after AnnexinVfluorescein isothiocyanate (FITC) and propidium iodide (PI) staining. Protein expressions of Bcl2 and Bax were studied by immunohistochemical staining and the ratios of Bcl2/Bax were calculated. RESULTS: The NR area in the AM group decreased significantly compared with the I/R group (t=4483, P<005). The apoptosis rates of cardiomyocyte in both I/R and AM groups were significantly higher than in the sham group (F=80090, P<001). Compared with that in the I/R group, the apoptosis rate of cardiomyocytes significantly decreased in the AM group (F=80090, P<001) and compared with that in sham group, expressions of Bcl2 and Bax protein increased in both I/R and AM groups (F=2901, P<001), (F=21445, P<001). Expression of Bcl2 protein in the AM group was higher (F=2901, P<001) and the expression of Bax protein was significantly lower (F=21445, P<001) than in the I/R group. Compared with that in the I/R group, ratios of Bcl2/Bax increased significantly (F=9761, P<001). CONCLUSION: Amiodarone may improve NR accompanied by inhibiting cardiomyocyte apoptosis induced by I/R injury in rabbits. The mechanism between antiapoptosis and reduction of the NR remains to be further clarified.

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备注/Memo

备注/Memo:
收稿日期：2012-12-03.基金项目：河北省科技计划项目基金资助（09276101D8） 通讯作者：信栓力，主任医师，主要从事心脏介入治疗研究 Email：xinkexuan@163.com
更新日期/Last Update: 2013-07-29