阿托伐他汀对冠状动脉微栓塞后非梗死区心肌炎症的影响
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2013年第5期
- 页码:
- 509-511523
- 栏目:
- 基础研究
- 出版日期:
- 2013-09-25
文章信息/Info
- Title:
- Roles of atorvastatin in myocardial inflammation of uninfarcted area following coronary microembolization
- Author(s):
- LI Shu-mei; LIU Yong-ping; HUANG Zhang-min; LIN Feng
- (Department of Cardiology, PLA 476 Hospital, Fuzhou General Hospital, Fuzhou 350002, Fujian, China)
- Keywords:
- atorvastatin; coronary artery; embolization; inflammatory reaction; rabbit
- 分类号:
- R972.6
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的:探讨阿托伐他汀干预治疗对冠状动脉微栓塞（CME）后非梗死区心肌炎症的影响。方法： 给清洁级雄性新西兰兔选择性经左前降支(LAD)注入微栓子颗粒悬液建立CME模型，将36只新西兰兔随机分为CME组（模型组）、阿托伐他汀（atrovastatin）干预组及对照组，每组12只(n=12)。于术后7 d处死，取左心室乳头段心肌经HE染色后，观察梗死心肌并检测梗死面积。用ELISA法及RT-PCR检测肿瘤坏死因子α（TNF-α）、白介素6（IL-6）及其基因在非梗死区（右室）心肌中的表达。结果： 前降支动脉微栓塞后，非梗死区右室心肌中白细胞浸润明显增多，TNF-α、IL-6蛋白及其基因的表达显著增强。与未处理组相比，阿托伐他汀可显著抑制CME后右室心肌中白细胞浸润及TNF-α、IL-6蛋白及其基因的表达(均P<0.01)。结论： 冠脉微栓塞后，炎症反应的激活不仅局限于梗死灶，且累及非梗死区心肌。阿托伐他汀可显著抑制CME后非梗死区心肌炎症反应。
- Abstract:
- AIM:To investigate the roles of atorvastatin in myocardial inflammation of uninfarcted area following coronary microembolization (CME). METHODS: CME model in rabbits was established by injecting homologous microthrombotic particle suspension into the left descending anterior branch (LAD). Thirty-six New Zealand rabbits were randomized to untreated CME group, atorvastatin-treated CME group and sham operation group (n=12/group). Rabbits were sacrificed on postoperative day 7. The morphological characteristics were evaluated in sections with H/E staining and protein and gene expressions of TNFα and IL-6 were semiquantitatively analyzed by ELISA and RT-PCR. RESULTS: LAD microembolization produced increased leukocyte infiltration and strong TNFα, IL-6 mRNA and protein expressions in uninfarcted right ventricular myocardium. Atorvastatin significantly suppressed the inflammatory cell infiltration and protein and gene expression of TNFα and IL-6 (P<0.01). CONCLUSION: Coronary microembolization produces myocardial inflammatory activation, which is not only confined to the microinfarcted zones but also the uninfarcted area. Atorvastatin markedly suppresses TNFα, IL-6 protein and gene expressions and effectively ameliorates myocardial inflammation in the uninfarcted area.
参考文献/References
[1]Heusch G,Kleinbongard P,B?se D,et al.Coronary microembolization:from bedside to bench and back to bedside[J].Circulation,2009,120(18):1822-1836.
[2]Liuzzo G,Santamaria M,Biasucci LM,et al.Persistent activation of nuclear factor kappa-B signaling pathway in patients with unstable angina and elevated levels of C-reactive protein evidence for a direct proinflammatory effect of azide and lipopolysaccharide-free C-reactive protein on human monocyte via nuclear factor kappa-B activation [J].J Am Coll Cardiol,2007,49(2):185-194.
[3]Skyschally A,Haude M,Dorge H,et al.Glucocorticoid treatment prevents progressive myocardial dysfunction resulting from experimental coronary microembolization[J].Circulation,2004,109(19):2337-2342.
[4]Farah R,Jubran F,Khamisy-Farah R.Effects of statins on oxidative stress and primed polymorphonuclear leukocytes in hyperlipidemic patients[J].Biotech Histochem,2012,87 (8):519-525.
[5]Li S,Zhong S,Zeng K,et al.Blockade of NF-κB by pyrrolidine dithiocarbamate attenuates myocardial inflammatory response and ventricular dysfunction following coronary microembolization induced by homologous microthrombi in rats[J].Basic Res Cardiol,2010,105(1):139-150.
[6]李淑梅,陈良龙,曾 凯,等.炎症因子在大鼠冠状动脉微栓塞后心肌组织中的表达及NF-κB抑制剂对其的影响[J].第二军医大学学报,2011,32(8):851-855.
[7]王佳兴,张玉顺,刘海莲,等.波生坦对高原性肺水肿的治疗作用[J].现代生物医学进展,2009,9(4):693-695.
[8]Patti G Pasceri V,Colonna G,et al.Atorvastatin pretreatment improves outcomes in patients with acute coronary syndromes undergoing early percutaneous coronary intervention: results of the ARMYDA-ACS randomized trial[J].J Am Coll Cardiol,2007,49(12):1272-1278.
备注/Memo
- 备注/Memo:
- 收稿日期：2013-03-25.基金项目：南京军区医学科技创新基金项目资助（09MA104） 作者简介：李淑梅,副主任医师,博士 Email: zdoctor@sohu.com
常用功能
统计/Statistics
- 摘要浏览/Viewed788
- 评论/Comments
