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|本期目录/Table of Contents|

虎杖苷对严重烫伤大鼠心肌细胞钙信号调控的作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2013年第6期
页码:
639-643
栏目:
基础研究
出版日期:
2013-11-25

文章信息/Info

Title:
Polydatin modulates calcium signals in thermally injured cardiomyocytes
作者:
邓建新1; 蓝莉芹1; 张翠翠1; 丁文文1; 薛 翔1; 刘文娟2
(1.南方医科大学病理生理学教研室、广东省医学休克微循环重点实验室，广东 广州510515；2.深圳大学医学院病理生理学教研室，广东 深圳518060)
Author(s):
DENG Jianxin1;  LAN Liqin1;  ZHANG Cuicui1;  DING Wenwen1;  XUE Xiang1;  LIU Wen juan2
(1.Department of Pathophysiology, Guangdong Key Lab for Shock and Microcirculation Research, Southern Medical University, Guangzhou 510515, Guangdong, China; 2.Department of Pathophysiology, School of Medicine, Shenzhen University, Shenzhen 518060, Guangdong, China)
关键词:
虎杖苷; 烫伤; 心肌细胞; 钙信号; 大鼠
Keywords:
polydatin;  thermal injury;  cardiomyocytes;  calcium signals;  rat
分类号:
R282.71
DOI:
-
文献标识码:
A
摘要:
目的:观察虎杖苷(polydatin，PD)对烫伤大鼠心功能损害的钙信号调控，并探讨其对烫伤大鼠心肌细胞保护作用的机制。方法:28只成年SD大鼠随机分为4组，即假手术组、烫伤组、PD组及烫伤+PD组，每组7只大鼠(n=7)。 各实验组使用Power lab系统进行实时心功能监测。手术后5 min通过静脉给予PD（10 mg/kg）,12 h后分离心室肌细胞，通过激光共聚焦显微镜观测PD对烫伤大鼠心室肌细胞钙火花发放频率、钙库水平、钙瞬变的作用。结果:与烫伤组比较，烫伤+PD组大鼠左室收缩压、左心室内压力上升的最大速率(±dP/dtmax)均显著增加、钙瞬变和心肌收缩力明显增强、使烫伤引起的钙库容量下降基本恢复到正常水平，烫伤引起的高频钙火花发放显著抑制(均P<0.01)，钙火花时程的增加也明显抑制(P<0.05)。结论: PD可以显著抑制烫伤引起的心肌细胞肌浆网钙漏流，恢复肌浆网钙库容量水平，增强收缩期钙瞬变和心肌收缩力，从而改善烫伤引起的心功能障碍。
Abstract:
AIM:To investigate the protective effect of polydatin (PD) on calcium signals in thermally injured cardiomyocytes. METHODS: Twenty eight adult Sprague Dawley rats were randomly divided into four groups (seven rats/group): shamoperation group, thermal injury group, PD group and thermal injury+PD group. Polydatin (10 mg/kg) was given intravenously 5 min after operation. Twelve hours after thermal injury, single myocytes were isolated from ventricular free wall enzymatically and loaded with Ca2+ indicator fluo4 AM. Ca2+ sparks and transients were recorded. RESULTS: PD significantly improved left ventricular systolic pressure (LVSP), +dP/dtmax and dP/dtmax of thermal injury cardiomyocytes (P<0.01). Polydatin significantly increased thermal injury systolic Ca2+ transients by about 26% and myocyte contractility (P<0.01), restored sarcoplasmic reticulum Ca2+ content (P<0.01), inhibited thermal injuryinduced high frequency and the duration of calcium sparks (P<0.05). CONCLUSION: Polydatin has the capability of preventing SR leak and restoring intracellular Ca2+ homeostasis in thermal traumatized hearts and ameliorates thermally induced cardiac dysfunction, thus exerting strong therapeutic effect against burngenerated cardiac dysfunction.

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备注/Memo

备注/Memo:
收稿日期：2013-03-28.基金项目：国家自然科学基金项目资助(81200122)；深圳大学自然科学基金项目资助(00035904) 通讯作者：刘文娟，讲师，主要从事心脏电生理及钙信号调控研究 Email：lwj0320@126.com 作者简介：邓建新，实验师，博士 Email：cheehsin@126.com
更新日期/Last Update: 2013-11-20