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|本期目录/Table of Contents|

新生大鼠心肌细胞的原代培养及鉴定

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2013年第6期
页码:
654-656
栏目:
基础研究
出版日期:
2013-11-25

文章信息/Info

Title:
Culture and identification of cardiomyocytes in neonatal rats
作者:
胡保奎; 耿召华; 祝善俊; 刘玉峰
(第三军医大学新桥医院心血管内科，重庆 400037)
Author(s):
HU Bao kui;  GENG Zhao hua;  ZHU Shan jun;  LIU Yu feng
(Department of Cardiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China)
关键词:
细胞培养; 心肌细胞; 大鼠
Keywords:
cell culture;  cardiomyocyte;  rat
分类号:
R329.2
DOI:
-
文献标识码:
A
摘要:
目的:探讨新生大鼠心肌细胞原代培养的方法，培养存活率高和纯度高的心肌细胞。方法:无菌条件下取出出生1~2 d的SD大鼠心脏，用2.5 g/L胰蛋白酶和 2.0 g/L Ⅱ型胶原酶等量混合液在37℃条件下分次消化心肌组织。以差速贴壁并将时间延长至90 min纯化心肌细胞，48 h后换液。结果： 将心肌细胞24 h贴壁生长，于2~3 d心肌细胞伸出伪足，形成细胞簇，同步搏动，频率约80~130次/min，存活率为(93.9±2.8)%，纯度为(91.9±2.2)%。结论:以本实验的方法培养的心肌细胞存活率高、纯度高，是一种较为理想的原代心肌细胞培养方法。
Abstract:
AIM:To develop a method of primary culture of neonatal rat cardiomyocytes with high survival cell rate and high purity. METHODS: Hearts of 1 to 2dayold neonatal Sprague Dawley rats were removed under sterile conditions with 0.25% trypsin and 02% collagenase type II at 37℃ under the conditions of graded digestion myocardial tissue. Differential adherence purification was applied. The time was extended to 90 min and the medium was changed after 48 h. RESULTS: Adherent growth of myocardial cells was observed at 24 h and pseudopodia growth at 2-3 days. Myocardial cells formed cell clusters and the synchronous beat frequency was about 80-130 times/min with survival rate of (93.9±2.8)% and purity of (91.9±2.2)%. CONCLUSION: We developed a method to culture myocardial cells with high survival cell rate and high purity.

参考文献/References

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[2]Marshall GE,Russell JA,Tellez JO,et al.Remodelling of human atrial K+ currents but not ion channel expression by chronic βblockade[J].Pfluger Arch,2012,463(4):537-548.
[3] 李 博,吴慧颖,朴虎林,等.新生大鼠心肌细胞原代培养方法的改良[J].中国实验诊断学,2012,16(2):200-203.
[4]刘嘉祺,孙云云.乳鼠心肌细胞原代培养方法[J].微量元素与健康研究,2012,29(4):4-6.
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备注/Memo

备注/Memo:
收稿日期：2013-05-14.基金项目：国家自然科学基金项目资助(81070093)；重庆市科技攻关计划项目资助(CSTC，2011AC5031) 通讯作者：耿召华，副教授，主要从事Rad蛋白相关研究 Email：gengzhh@tom.com 作者简介：胡保奎，硕士生 Email：641117506@qq.com
更新日期/Last Update: 2013-11-20