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|本期目录/Table of Contents|

丙酮酸对缺血/再灌注大鼠心肌的保护作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2014年第2期
页码:
143-146
栏目:
基础研究
出版日期:
2014-01-20

文章信息/Info

Title:
Protection of pyruvate on heart against ischemia/reperfusion injury
作者:
徐 杰张 星秦兴华杨 璐邢 媛高 峰
(第四军医大学基础医学院生理学教研室,陕西 西安 710032)
Author(s):
XU Jie ZHANG Xing QIN Xing-hua YANG Lu XING Yuan GAO Feng
(Department of Physiology, School of Basic Medical Sciences, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
丙酮酸缺血/再灌注心脏功能大鼠
Keywords:
pyruvate ischemia/reperfusion cardiac function rat
分类号:
Q555.7
DOI:
-
文献标识码:
A
摘要:
目的:探讨丙酮酸(Pyr)对缺血/再灌注(I/R)大鼠心肌的影响及其可能的机制。方法: 30只SD成年大鼠随机分为假手术(Sham)组、I/R组及I/R+Pyr组,每组10只。I/R+Pyr组大鼠于再灌前5 min,开始持续性静脉灌注Pyr 2 h[25 mg/(kg·h)]。再灌注2 h后,利用多道生理记录仪检测大鼠在体血流动力学指标:平均动脉压(MABP)、左室收缩压(LVSP)及左室最大收缩、舒张末压微分(±LV dP/dtmax)。采用Western blot方法检测磷酸化-JNK(p-JNK)和总的JNK(t-JNK)表达。用原位缺口末端标记法(TUNEL)评价心肌细胞的凋亡。结果: I/R组的MABP、LVSP、±LV dP/dtmax显著低于Sham组(P<0.01),Pyr干预可增加I/R后MABP、LVSP及±LV dP/dtmax的水平(P<0.05)。与Sham组相比,I/R组大鼠左心室p-JNK的水平明显增高(P<0.01);而Pyr可降低大鼠左心室p-JNK的水平(P<0.01),并抑制心肌细胞凋亡(P<0.05)。结论: Pyr可改善I/R大鼠心肌的功能,抑制心肌细胞凋亡,其机制可能与抑制JNK信号的激活有关。
Abstract:
AIM:To investigate the effect of pyruvate on myocardial ischemia/reperfusion (I/R) injury and the underlying mechanism. METHODS: The animal model of myocardial I/R injury was induced by 30 min of left anterior descending coronary occlusion followed by 2 h of reperfusion. Thirty rats were randomly divided into three groups: sham group, I/R group and I/R plus pyruvate treatment (I/R+Pyr) group. Pyruvate was intravenously infused at 25 mg/(kg·h) for 2 h, 5 min before reperfusion. Hemodynamic parameters (including LVSP, MABP and ±LV dP/dtmax) phosphorylation and total levels of JNK were determined and cardiomyocyte apoptosis was evaluated by TUNEL staining. RESULTS: LVSP, MABP and ±LV dP/dtmax decreased in I/R group and pyruvate treatment reversed these effects. p-JNK level increased in I/R group compared with that in sham group. Pyruvate inhibited p-JNK activation but did not change JNK expression. The index of apoptotic cardiomyocytes decreased with pyruvate treatment compared with that in I/R group. CONCLUSION: Pyruvate improves the myocardial function and inhibits myocardial apoptosis, which may result from the inhibition of JNK phosphorylation in the rat myocardial I/R model.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期:2013-11-11.
通讯作者:高峰,教授,主要从事胰岛素抵抗与心血管疾病研究Email:fgao@fmmu.edu.cn
作者简介:徐杰,硕士生Email: xujiefmmu@126.com
更新日期/Last Update: 2014-01-16