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Ghrelin 通过抑制ERS减轻ISO所致的心肌损伤和心肌细胞凋亡

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2014年第3期
页码:
249-253
栏目:
基础研究
出版日期:
2014-03-20

文章信息/Info

Title:
Ghrelin reduces isoproterenol-induced myocardial injury and cardiomyocyte apoptosis via inhibiting endoplasmic reticulum stress
作者:
张改改1; 蔡怀秋2; 张金胜3; 宁 明1; 吴 扬1; 齐永芬3; 尹新华2
(1.清华大学第一附属医院、北京华信医院干部病房，北京 100016；
2.哈尔滨医科大学第一附属医院心内科，黑龙江 哈尔滨 150086；
3.北京大学医学部教育部分子心血管病学重点实验室，北京 100191)
Author(s):
ZHANG Gai gai1;  CAI Huai qiu2;  ZHANG Jin sheng3;  NING Ming1;  WU Yang1;  QI Yong fen3;  YIN Xin hua2
(1.Cadre Ward, Beijing Huaxin Hospital & First Affiliated Hospital, Tsinghua University, Beijing 100016, China;
2.Department of Cardiology, First Affiliated Hospital, Harbin Medical University, Harbin 150086, Heilongjiang, China;
3.Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, Peking University Health Science Center, Beijing 100191, China)
关键词:
ghrelin; 异丙基肾上腺素; 心肌疾病; 内质网应激; 凋亡
Keywords:
ghrelin;  isoproterenol;  cardiomyopathy;  endoplasmic reticulum stress;  apoptosis
分类号:
Q25；R542 2
DOI:
-
文献标识码:
A
摘要:
目的:探讨ghrelin(G)减轻异丙基肾上腺素(isoproterenol，ISO)所致心肌损伤和凋亡是否与其抑制心肌内质网应激（endoplasmic reticulum stress，ERS）有关。方法:将29只雄性SpragueDawley(SD)大鼠随机分为对照(Con)组、ISO组和ISO+G组。Con组皮下注射生理盐水，ISO组皮下注射ISO，ISO+G组皮下注射ISO前2 d皮下注射ghrelin。采用生理记录仪测定各组大鼠心功能。以HE染色及血浆乳酸脱氢酶(LDH)及肌酸激酶MB(CK-MB)活性的测定评估心肌损伤的程度。用TUNEL法测定心肌细胞的凋亡。用Western blot检测各组心肌组织中ERS标志性分子C/EBP同源蛋白(C/EBPhomologous protein，CHOP)和caspase-12的表达。结果:外源性ghrelin可显著缓解ISO所致大鼠的心功能不全，减轻ISO造成的心肌损伤，减少心肌细胞凋亡，并降低CHOP 和剪切的caspase-12的表达。结论:ghrelin通过抑制ERS可减轻ISO所致心肌损伤和心肌细胞凋亡。
Abstract:
AIM:To investigate whether ghrelin (G)-reduced myocardial injury and cardiomyocyte apoptosis induced by isoproterenol (ISO) is related to the inhibition of endoplasmic reticulum stress (ERS). METHODS: Twenty-nine male Sprague Dawley (SD) rats were randomly divided into control group (n=9),ISO group (n=11) and ISO+G group (n=9). Rats in control (Con) group were subcutaneously injected with normal saline. Rats in ISO group were injected with ISO and rats in ISO+G group were injected with ghrelin 2 days before injection of ISO. Hemodynamic parameters of the rats in each group were recorded by the Power Lab system. Myocardial tissue injury was assessed by HE staining and activity determination of plasma lactate dehydrogenase (LDH) and creatinine kinase (CK)-MB. Cardiomyocyte apoptosis was detected by TUNEL method and the expression of ERS markers C/EBP-homologous protein (CHOP) and cleaved caspase-12 in myocardial tissue was detected by Western blot. RESULTS: Exogenous ghrelin alleviated heart dysfunction, reduced myocardial injury and cardiomyocyte apoptosis and inhibited overexpressions of CHOP and cleaved caspase-12. CONCLUSION: Ghrelin reduced ISO-induced myocardial injury and cardiomyocyte apoptosis via inhibiting ERS.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2013-07-25.
基金项目：国家自然科学基金项目资助（81000046）
作者简介：张改改，主治医师，博士 Email: zhanggaigai9@163.com
更新日期/Last Update: 2014-03-21