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|本期目录/Table of Contents|

STZ诱导的2型糖尿病大鼠心肌组织TRPC7的表达上调

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2014年第4期
页码:
403-407
栏目:
基础研究
出版日期:
2014-04-25

文章信息/Info

Title:
Expression of cardiac TRPC7 channels increases in streptozotocin-induced diabetic rats
作者:
张海军1; 崔龙彪1; 岳 原2; 杜 成1; 常耀明1; 史 娟3; 谢满江1
(第四军医大学：1.航空航天医学教育部重点实验室、航空航天医学系，3.人体解剖与组织胚胎学教研室，陕西 西安 710032；
2.国营长风机器厂职工医院内科，甘肃 兰州730070)
Author(s):
ZHANG Hai-jun1;  CUI Long-biao1;  YUE Yuan2;  DU Cheng1;  CHANG Yao-ming1;  SHI Juan3;  XIE Man-jiang1
(1.Key Laboratory of Aerospace Medicine of Ministry of Education & Department of Aerospace Physiology, 3.Department of Anatomy and KK Leung Brain Research Centre, Fourth Military Medical University, Xi’an 710032, Shaanxi, China;
2.Department of Internal Medicine, Gansu National Changfeng Machinery Factory Hospital, Lanzhou 730070, Gansu, China)
关键词:
链脲佐菌素; 糖尿病; 心肌细胞; TRPC7; 大鼠
Keywords:
streptozotocin;  diabetes;  cardiac;  TRPC7;  rat
分类号:
R587.1
DOI:
-
文献标识码:
A
摘要:
目的:观察2型糖尿病（T2DM）大鼠心肌组织损伤过程中，TRPC7的表达变化。方法: 以高脂饲料喂养并辅以腹腔注射小剂量链脲佐菌素（Streptozotocin,STZ）来建立T2DM大鼠模型，采用蛋白印迹及免疫组化染色方法检测TRPC7在T2DM大鼠心肌组织的表达变化。结果: ①T2DM大鼠造模成功且T2DM大鼠心肌组织发生损伤性变化：与正常组大鼠相比，T2DM组大鼠血糖明显升高且体重显著降低(P<0.05)；T2DM组大鼠心脏体积与质量显著减小(P<0.05)；T2DM组大鼠心脏质量指数(heart weight index,HWI)明显高于正常组(P<0.05)；②在T2DM大鼠心肌损伤过程中TRPC7表达上调：TRPC7在正常及T2DM大鼠心肌组织中均有表达，但与正常大鼠相比，T2DM大鼠心肌组织TRPC7蛋白的表达显著增高（P<0.05）。结论: 首次证实在损伤的T2DM大鼠心肌组织中，TRPC7的表达显著上调。
Abstract:
AIM:To investigate the change of cardiac TRPC7 expression in streptozotocin (STZ)-induced type 2 diabetic rats. METHODS: STZ-induced type 2 diabetic rat models were established and the expression of cardiac TRPC7 channels was evaluated by immunofluorescence and Western blot. RESULTS: 1) The impaired heart was found in STZ-induced T2DM rats. 2) The expression of cardiac TRPC7 significantly increased in STZ-induced T2DM rats as compared with that in control rats. CONCLUSION: The present work makes a novel finding that the expression of cardiac TRPC7 channels significantly increased in the impaired hearts of STZ-induced type 2 diabetic rats.

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备注/Memo

备注/Memo:
收稿日期：2014-01-16.基金项目：国家自然科学基金项目资助(31070768，31270904，81171871) 通讯作者：谢满江，副教授，主要从事特殊环境因素与心血管疾病研究 Email:xiemanjiang@163.com 作者简介：张海军，硕士生 Email:zhjbeijing2008@163.com
更新日期/Last Update: 2014-05-06