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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2014年第5期

页码:

514-519

栏目:

基础研究

出版日期:

2014-05-25

文章信息/Info

Title:

Study on Smad signal pathway of cardiomyocyte hypertrophy induced by TGF-β1 or AngII in rats

作者:

石永英¹; 卢俊江²; 陈广原³; 刘世明⁴; 陈敏生⁴

(1.广州医科大学附属第一医院老年科、广州心血管病研究所，广东 广州 510120；
2.遵义医学院第五附属 医院(珠海)医院，广东 珠海 519090；
3.广州医科大学附属第一医院老年科，广东 广州 510120；
4.广州医科大学附属第二医院心内科、广州心血管病研究所，广东 广州 510260)

Author(s):

SHI Yong-ying¹;  LU Jun-jiang²;  CHEN Guang-yuan³;  LIU Shi-ming⁴;  CHEN Min-sheng⁴

1.Department of Geriatrics, Guangzhou Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, Guangdong, China;etc

关键词:

转化生长因子-β1; 血管紧张素II; 心肌细胞肥大; Smad; 大鼠

Keywords:

transforming growth factor beta 1;  cardiomyocte hypertrophy;  angiotensin II;  rat

分类号:

R542.2

DOI:

-

文献标识码:

A

摘要:

目的:探讨在TGF-β1及AngⅡ分别诱导的大鼠心肌细胞肥大中Smad信号途径中Smad2蛋白的表达。方法： 分别建立TGF-β1及AngⅡ诱导的大鼠心肌细胞肥大模型，分为正常对照组、TGF-β1组以及AngⅡ组。以碘化丙啶(PI)染色标记法检测心肌细胞中RNA的表达量以间接反映心肌细胞肥大。以实时荧光定量PCR检测心肌细胞肥大相关肌球蛋白重链β亚型（β-MHC）的表达。以Western blot检测心肌细胞中磷酸化Smad2信号蛋白的表达。结果： TGF-β1及AngⅡ诱导的培养心肌细胞中肥大相关蛋白β-MHC的表达均明显高于对照组（P<0.01）。PI染色检测表明，TGF-β1组及AngⅡ组PI的含量明显增高(P<0.01)。与对照组相比，TGF-β1及AngⅡ均可明显上调磷酸化Smadz(p-Smad2)的表达（P<0.01）。TGF-β1组与AngⅡ组p-Smad2表达峰值相比无明显差异，但达峰的时间有所不同。结论： TGF-β1及AngⅡ单独均可诱导心肌细胞肥大，在此过程中p-Smad2蛋白的表达明显增加，TGF-β1与AngⅡ促进p-Smad2蛋白表达作用无明显差异。

Abstract:

AIM:To explore the mechanism of TGF-β1 or AngII activated cardiomyocyte hypertrophy in regard to Smadpathway. METHODS: Fresh isolated neonatal rat cardiomyocytes induced by normal, TGF-β1 and AngII groups were used to examine the formation of cell pathway. p-Smad2 protein was measured by Western blot. Hypertrophy was measured by PI staining and RNA expressions were detected by real-time PCR. RESULTS: TGF-β1 and AngII induced cardiomyocyte hypertrophy and Smad protein in cardiomyocytes was stimulated by TGF-β1 or AngII. Expression of β-MHC was obviously higher than that in the control group (P<0.01). PI staining of TGF-β1 group and AngII group were significantly increased compared with the control group (P<0.01). Compared with the control group, p-Smad2 proteins in cardiomyocytes were stimulated by TGF-β1 or AngII. In the TGF-β1 group and AngII group there were no significant differences in the peak time. CONCLUSION: TGF-β1 and AngII activate the Smad signaling system in cardiomyocyte hypertrophy in vitro.

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备注/Memo

备注/Memo:

收稿日期：2013-04-23.
基金项目：广州市科技局计划支撑项目资助（2009Z1-E271）
通讯作者：陈敏生，教授，主要从事高血压病的发病机制及并发症防治研究 Email：shiyongying121@163.com
作者简介：石永英，副主任医师，博士 Email：shiyongying121@163.com

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更新日期/Last Update: 2014-06-05