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TGF-β1及AngⅡ对大鼠心肌细胞肥大的信号转导通路作用研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2014年第5期
页码:
514-519
栏目:
基础研究
出版日期:
2014-05-25

文章信息/Info

Title:
Study on Smad signal pathway of cardiomyocyte hypertrophy induced by TGF-β1 or AngII in rats
作者:
石永英1卢俊江2陈广原3刘世明4陈敏生4
(1.广州医科大学附属第一医院老年科、广州心血管病研究所,广东 广州 510120;
2.遵义医学院第五附属 医院(珠海)医院,广东 珠海 519090;
3.广州医科大学附属第一医院老年科,广东 广州 510120;
4.广州医科大学附属第二医院心内科、广州心血管病研究所,广东 广州 510260)
Author(s):
SHI Yong-ying1 LU Jun-jiang2 CHEN Guang-yuan3 LIU Shi-ming4 CHEN Min-sheng4
1.Department of Geriatrics, Guangzhou Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangzhou Medical University, Guangzhou 510120, Guangdong, China;etc
关键词:
转化生长因子-β1血管紧张素II心肌细胞肥大Smad大鼠
Keywords:
transforming growth factor beta 1 cardiomyocte hypertrophy angiotensin II rat
分类号:
R542.2
DOI:
-
文献标识码:
A
摘要:
目的:探讨在TGF-β1及AngⅡ分别诱导的大鼠心肌细胞肥大中Smad信号途径中Smad2蛋白的表达。方法: 分别建立TGF-β1及AngⅡ诱导的大鼠心肌细胞肥大模型,分为正常对照组、TGF-β1组以及AngⅡ组。以碘化丙啶(PI)染色标记法检测心肌细胞中RNA的表达量以间接反映心肌细胞肥大。以实时荧光定量PCR检测心肌细胞肥大相关肌球蛋白重链β亚型(β-MHC)的表达。以Western blot检测心肌细胞中磷酸化Smad2信号蛋白的表达。结果: TGF-β1及AngⅡ诱导的培养心肌细胞中肥大相关蛋白β-MHC的表达均明显高于对照组(P<0.01)。PI染色检测表明,TGF-β1组及AngⅡ组PI的含量明显增高(P<0.01)。与对照组相比,TGF-β1及AngⅡ均可明显上调磷酸化Smadz(p-Smad2)的表达(P<0.01)。TGF-β1组与AngⅡ组p-Smad2表达峰值相比无明显差异,但达峰的时间有所不同。结论: TGF-β1及AngⅡ单独均可诱导心肌细胞肥大,在此过程中p-Smad2蛋白的表达明显增加,TGF-β1与AngⅡ促进p-Smad2蛋白表达作用无明显差异。
Abstract:
AIM:To explore the mechanism of TGF-β1 or AngII activated cardiomyocyte hypertrophy in regard to Smadpathway. METHODS: Fresh isolated neonatal rat cardiomyocytes induced by normal, TGF-β1 and AngII groups were used to examine the formation of cell pathway. p-Smad2 protein was measured by Western blot. Hypertrophy was measured by PI staining and RNA expressions were detected by real-time PCR. RESULTS: TGF-β1 and AngII induced cardiomyocyte hypertrophy and Smad protein in cardiomyocytes was stimulated by TGF-β1 or AngII. Expression of β-MHC was obviously higher than that in the control group (P<0.01). PI staining of TGF-β1 group and AngII group were significantly increased compared with the control group (P<0.01). Compared with the control group, p-Smad2 proteins in cardiomyocytes were stimulated by TGF-β1 or AngII. In the TGF-β1 group and AngII group there were no significant differences in the peak time. CONCLUSION: TGF-β1 and AngII activate the Smad signaling system in cardiomyocyte hypertrophy in vitro.

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备注/Memo

备注/Memo:
收稿日期:2013-04-23.
基金项目:广州市科技局计划支撑项目资助(2009Z1-E271)
通讯作者:陈敏生,教授,主要从事高血压病的发病机制及并发症防治研究 Email:shiyongying121@163.com
作者简介:石永英,副主任医师,博士 Email:shiyongying121@163.com
更新日期/Last Update: 2014-06-05