老龄载脂蛋白E基因敲除小鼠血脂及病理组织学观察
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2014年第6期
- 页码:
- 635-636640
- 栏目:
- 基础研究
- 出版日期:
- 2014-06-25
文章信息/Info
- Title:
- Observation of serum lipid and histopatholoical changes in elderly mice with apolipoprotein E knockout gene
- Author(s):
- LI Feng-qing; SUN Ting-ting; WANG Jun-xiao; LUO Shan-shun
- (Department of Cardiology, First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China)
- 关键词:
- 载脂蛋白E基因敲除小鼠; 血脂; 病理改变
- Keywords:
- ApoE gene knockout mice; serum lipids; pathological changes
- 分类号:
- R543
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的:观察用普食喂养而非以往研究中的高脂饮食喂养的载脂蛋白E基因敲除小鼠（ApoE-/-）的血脂及病理组织学变化特点。方法： 各选取20只8周龄雄性小鼠和同龄同性C57BL/6J小鼠为对照，普食喂养6~8个月，测定血清中总胆固醇(TC)、三酰甘油（TG）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）的含量。常规制备主动脉的病理切片，进行HE染色，观察病理学变化。结果： ApoE-/-小鼠血清中TC、TG、LDL含量明显高于对照组（P<0.05），且ApoE-/-小鼠主动脉根部病理切片明显脂质斑块形成。结论： 普食喂养8月龄ApoE-/-小鼠是研究动脉粥样硬化(AS)的理想动物模型，为AS性心血管疾病研究者提供了参考资料。
- Abstract:
- AIM:To observe serum lipid and histopathological changes in ApoE gene knockout mice fed a normal diet instead of a high-fat diet. METHODS: Twenty eight-week-old mice (male) with ApoE gene knockout were selected and 20 C57BL/6J mice (male) were used as controls. The mice in both groups were fed normal diets for 6-8 months and the levels of TC, TG and LDLwere measured in the two groups. Routine aortic pathological productions were stained by HE to observe the pathological changes. RESULTS: Levels of TC, TG and LDL-c in mice with ApoE knockout gene were significantly higher than those in control group (P<0.05) and atherosclerotic plaques formed in ApoE gene knockout mice. CONCLUSION: ApoE gene knockout mice fed normal diets for 8 months are the ideal animal models for studies on atherosclerotic plaque research.
参考文献/References
[1]翟永志,晏沐阳.载脂蛋白E 基因敲除小鼠用于动脉粥样硬化研究的进展[J].中国比较医学杂志,2008,18(2):55-58.
[2]Plump AS,Smith JD,Hayek T,et al.Severe hypercholesterolemia and atherosclerosis in apolipoprotein E-deficient mice created by homologous recombination in ES cells[J]. Cell,1992,71(2):343-353.
[3]Zhang SH,Reddick RL,Piedrahita JA,et al.Spont aneous hypercholest erolemia and art erial lesions in mice lacking aopolipoprotein E[J].Science,1992,258(10):468-471.
[4]刘雪梅,吴符火.几类高脂血症动物模型的比较[J].中西医结合学报,2004,2(2):132-134.
[5]康爱君，田 枫，张 阔，等.载脂蛋白E 基因缺陷(C57BL/6J-ApoE)小鼠血脂及病理组织学观察[J].中国比较医学杂志,2006,16(2):85-88.
[6]景志杰，郭民.载脂蛋白E 基因敲除小鼠血脂及病理组织学观察[J].临床医学工程,2011,18(6):822-823.
[7]黄朝晖,吴雄飞,赵洪雯,等.ApoE~(-/-)小鼠肾动脉狭窄及肾损害特点观察[J].第三军医大学学报,2007,29(19):1844-1847.
备注/Memo
- 备注/Memo:
- 收稿日期：2014-01-26.
基金项目：国家自然科学基金项目资助（81070216）；国家自然科学基金项目资助（81270366）
作者简介：李凤卿，硕士生Email：lifengqing2002@163.com
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更新日期/Last Update: 2014-07-10