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3-氨基苯甲酰胺对大鼠心肌梗死后心力衰竭心肌细胞细胞因子、多聚ADP核糖聚合酶和凋亡诱导因子蛋白表达的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2015年第2期
页码:
125-129
栏目:
基础研究
出版日期:
2014-10-25

文章信息/Info

Title:
Effects of 3-aminobenzamide on expression of cytokine, poly(ADP-ribose) polymerase and apoptosis-inducing factor on cardiocytes in rats with heart failure after myocardial infarction
作者:
赵玉娟; 武英彪; 陈彦东; 赵继义; 洪晓剑; 王付曼
(哈尔滨医科大学附属第一医院心内科，黑龙江 哈尔滨 150001)
Author(s):
ZHAO Yu-juan;  WU Ying-biao;  CHEN Yan-dong;  ZHAO Ji-yi;  HONG Xiao-jian;  WANG Fu-man
(Department of Cardiology, First Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China)
关键词:
多聚ADP核糖聚合酶; 3-氨基苯甲酰胺; 心力衰竭
Keywords:
poly(ADP-ribose) polymerase;  3-aminobenzamide;  heart failure
分类号:
R541.6
DOI:
-
文献标识码:
A
摘要:
目的:研究多聚ADP核糖聚合酶（PARP）阻滞剂3-氨基苯甲酰胺（3-AB）对心力衰竭（HF）大鼠心肌白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）水平及对PARP和凋亡诱导因子（AIF）蛋白表达的影响。方法:结扎135只成年雄性Wistar大鼠冠状动脉前降支6周制备HF模型，随机分为HF组、3-AB治疗组（3-AB组）和Sham组。治疗组应用3-AB治疗(30 mg/kg)。治疗2、4、6周后采用放射免疫法测定大鼠心肌IL-10、TNF-α水平，采用免疫组化方法检测心肌PARP和AIF蛋白表达变化。结果:IL-10水平变化：与Sham组相比，HF组各时间点明显增高（P<0.05），3-AB组较HF组各相同时间点明显降低（P<0.05）；TNF-α水平：HF组各时间点均较Sham组明显增高（P<0.05），而3-AB组较HF组各相同时间点明显降低（P<0.05）。PARP蛋白表达：HF组各时间点均较Sham组增加（P<0.05），3-AB组各时间点均较HF组明显减少（P<0.05）。AIF蛋白表达：HF组各时间点较Sham组增加，3-AB组各时间点较HF组明显减少（P<0.05）。结论:3-AB可抑制IL-10、TNF-α的释放；抑制PARP和AIF蛋白表达,从而抑制炎症反应和细胞凋亡，对HF大鼠心肌细胞发挥保护作用。
Abstract:
AIM:To investigate the effects of 3-aminobenzamide (3-AB) on interleukin-10 (IL-10), levels of tumor necrosis factor-α (TNF-α) and expression of PARP and apoptosis-inducing factor (AIF) on cardiocytes in rats with heart failure (HF). METHODS: One hundred and thirty-five adult Wistar rats underwent 6-w left anterior descending artery ligation to be models of HF. Rats were divided into HF group, 3-AB group and sham-operated group. Rats in 3-AB group received 3-AB (30 mg/kg) by intraperitoneal injection. Myocardial IL-10 and TNF-α levels in the rats were measured by radioimmunity and changes of expressions of PARP and AIF protein were measured by immunohistochemical staining in each group after treatment with 3-AB at 2-, 4- and 6-w. RESULTS: Compared with those in sham-operated rats, myocardial IL-10 levels and myocardial TNF-α levels in HF group increased 2-, 4- and 6-w after treatment and compared with those in the HF groups, both markedly decreased at the same time points (P<0.05). Compared with those in sham groups, expressions of PARP and AIF increased in HF groups and expressions of PARP and AIF were significantly suppressed in 3-AB in positive cells at the same time points (P<0.05). CONCLUSION: The present findings indicate that 3-AB could lower IL-10 and TNF-α concentrations and inhibit inflammatory reaction and apoptosis by decreasing PARP and AIF expressions, thus protecting cardiocytes in rats with HF.

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备注/Memo

备注/Memo:
收稿日期：2014-06-17.
基金项目：黑龙江省教育厅科学技术研究项目资助（11531210）
作者简介：赵玉娟，副主任医师，博士 Email:zhaoyujuan1966@sohu.com
更新日期/Last Update: 2014-11-18