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|本期目录/Table of Contents|

冠心病患者中TIPE2表达水平及其与MCP-1、MMP-9的相关性

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2015年第3期
页码:
274-277
栏目:
临床研究
出版日期:
2015-01-11

文章信息/Info

Title:
Relationship between the level of TIPE2 mRNA and level of monocyte chemotactic factor 1, matrix metalloproteinases 9 in patients with coronary heart disease
作者:
王 东1; 王 娟2; 李小梅2; 程艳丽1
（滨州医学院：1.附属医院心血管内科，2.临床学院，山东 滨州 256600）
Author(s):
WANG Dong1;  WANG Juan2;  LI Xiao-mei2;  CHENG Yan-li1
(1.Department of Cardiology, Affiliated Hospital, 2.Clinical College, Binzhou Medical College, Binzhou 256600, Shandong, China)
关键词:
冠状动脉疾病; 肿瘤坏死因子-α诱导蛋白-8样分子-2; 单核细胞趋化因子-1; 基质金属蛋白酶-9
Keywords:
coronary disease;  tumor necrosis factor-α induced protein 8 like 2;  monocyte chemotactic protein-1;  matrix metalloproteinases-9
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的:观察和分析冠心病患者中肿瘤坏死因子-α诱导蛋白-8样分子-2(tumor necrosis factor-α induced protein 8 like 2,TIPE2)表达水平及其与单核细胞趋化因子-1(MCP-1)和基质金属蛋白酶-9(MMP-9)的相关性。方法:2010年12月~2012年11月在我院行冠脉造影检查确诊冠心病患者100例，并根据冠脉病变的支数分组，并入选同时期在我院行冠脉造影术明确无冠脉狭窄的非冠心病组(30例)。采用聚合酶链式反应（PCR）测定外周血单个核细胞中TIPE2的基因水平，采用双抗体夹心ELISA 法测定血清中MCP-1和MMP-9水平。结果:①外周血单个核细胞TIPE2基因表达水平测定的结果：冠脉病变患者PBMC中TIPE2的基因表达水平明显低于非冠心病组，双支病变组低于单支病变组，多支病变组明显低于单支病变组和双支病变组，差异均具有统计学意义（P<0.01）；②TIPE2基因表达水平与MCP-1、MMP-9水平呈负相关,相关系数分别为r=-0.560,r=-0.569，均P<0.05）。结论:冠心病患者中TIPE2表达水平随冠脉病变血管支数的增加而降低，而且与MCP-1和MMP-9呈负相关。
Abstract:
AIM:To observe and analyze the relationship between tumor necrosis factor-α induced protein 8 like 2 (TIPE2) level, monocyte chemotactic factor-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) in patients with coronary heart disease (CHD). METHODS: One hundred coronary heart disease patient specimens were used for detection of TIPE2 expression and the level of MCP-1 and MMP-9 in serum. The study comprised patients who had coronary angiography from 2010-12 to 2011-11 and 30 patients served as the control group during the same period. The CHD group was divided into three subgroups: single lesion group (34), double lesion group (33), and multivessel lesion group (33). RESULTS: ① TIPE2 mRNA expression level in peripheral blood mononuclear cell: Compared with non-CHD group, the level of TIPE2 mRNA expression was significantly reduced in CHD group. TIPE2 level in double lesion group was lower than in the single lesion group. In the multivessel lesion group it was lower than in the single lesion group and double branch lesion group. TIPE2 level was significantly lower with statistically significant differences (P<0.01). ② TIPE2 level was negatively related to the level of MCP-1 and MMP-9. The correlation coefficient, respectively, was r=0.560, r=0.569, P<0.05). CONCLUSION: The level of TIPE2 in patients with CHD decreased with the increase of the coronary pathological changes vascular count and is negatively correlated with the level of MCP-1 and MMP-9.

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备注/Memo

备注/Memo:
收稿日期：2014-09-22.
通讯作者：王东，副教授，主要从事高血压及其并发症防治研究Email：binyiwangdong@126.com共同第一作者：王娟，硕士生Email：wangjuansddz@126.com
更新日期/Last Update: 2015-02-03