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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2015年第4期

页码:

422-426

栏目:

基础研究

出版日期:

2015-04-20

文章信息/Info

Title:

Effect of aerobic exercise on cardiac inflammation in spontaneously hypertensive rats and the underlying mechanisms

作者:

侯作旭¹; 米春娟²; 徐 杰¹; 邢 媛¹; 张 圆¹; 高 峰¹

(1.第四军医大学基础医学院生理学教研室，陕西 西安 710032；
2.西安科技大学体育部，陕西 西安 710054)

Author(s):

HOU Zuo-xu¹;  MI Chun-juan²;  XU Jie¹;  XING Yuan¹;  ZHANG Yuan¹;  GAO Feng¹

(1.Department of Physiology, School of Basic Medical Sciences, Fourth Military Medical University, Xi’an 710032, Shaanxi, China；
2.P.E. Department, Xi’an University of Science and Technology, Xi’an 710054, Shaanxi, China)

关键词:

有氧运动; 炎症; 叉头框蛋白1; 自发性高血压大鼠

Keywords:

aerobic exercise;  inflammation;  forkhead box O1;  spontaneously hypertensive rats

分类号:

R87

DOI:

-

文献标识码:

A

摘要:

目的 探讨有氧运动对自发性高血压大鼠（SHR）心脏炎症的调节及其机制。方法 8周龄雄性SHR大鼠和正常血压对照WKY大鼠各16只，将SHR大鼠随机分为SHR安静组和SHR运动组；将WKY大鼠随机分为WKY安静组和WKY运动组，每组8只。其中运动组大鼠进行8周无负重游泳运动。采用超声心动仪检测心脏功能，用ELISA试剂盒检测心脏炎症因子TNF-α和IL-1β的含量，Western blot法检测心脏叉头框蛋白1（FoxO1）、p65 NF-κB、磷酸化Akt（p-Akt）和总Akt（t-Akt）的表达。结果 与WKY安静组大鼠相比，SHR安静组大鼠左心室p65 NF-κB的表达以及炎症因子TNF-α、IL-1β的含量及FoxO1表达明显升高（P<0.05，P<0.01），p-Akt的水平下降（P<0.05）；与SHR安静组相比，8周有氧运动能够增强SHR大鼠心脏功能，降低p65 NF-κB的表达以及炎症因子TNF-α和IL-1β的含量（P<0.05），同时降低FoxO1表达（P<0.05），提高p-Akt的水平（P<0.05）。8周有氧运动同样能够降低WKY大鼠心脏FoxO1表达（P<0.05），提高p-Akt的水平（P<0.05）。结论 8周无负重游泳运动能够降低SHR大鼠心脏炎症因子TNF-α和IL-1β的含量，其可能与加强Akt磷酸化水平和下调FoxO1表达有关，确切机制有待进一步研究。

Abstract:

AIM To explore the effect of aerobic exercise on cardiac inflammation in spontaneously hypertensive rats (SHR) and the underlying mechanisms. METHODS Sixteen 8-week-old male SHR and 16 age-matched male normotensive Wistar-Kyoto (WKY) rats were randomly divided into SHR exercise group, SHR rest group, WKY exercise group and WKY rest group. Rats in exercise groups were subjected to an 8-week free-of-loading swim training session (1 h/day, 5 days/week). Cardiac functions were monitored by echocardiogram and the levels of TNF-α and IL-1β were determined by ELISA. FoxO1, p65 NF-κB, phosphorylated and total Akt were determined by Western blot analysis. RESULTS The ratio of phosphorylated Akt/total Akt was lower and the levels of FoxO1, p65 NF-κB, TNF-α and IL-1β were higher in SHR rest group than those in WKY rest group, whereas no difference of cardiac functions was observed between the two groups. Eight-week free-of-loading swim training increased the ratio of phosphorylated Akt/total Akt, reduced FoxO1 and p65 NF-κB expression and attenuated levels of TNF-α and IL-1β in SHR. Moreover, 8-week free-of-loading swim training also increased the ratio of phosphorylated Akt/total Akt and reduced FoxO1 expression in WKY rats. CONCLUSION Aerobic exercise significantly attenuates cardiac inflammation in spontaneously hypertensive rats, which may result from the increase in phosphorylated Akt and decrease in FoxO1 expression.

参考文献/References

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备注/Memo

备注/Memo:

收稿日期：2014-11-06.
基金项目：国家自然科学基金项目资助（81270301；81370388）
通讯作者：张圆，讲师，主要从事心血管炎症机制研究 Email：zhangyuan@fmmu.edu.cn
作者简介：侯作旭，硕士生 Email：houzuoxu@163.com

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更新日期/Last Update: 2015-04-22