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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2015年第4期

页码:

459-463

栏目:

临床研究

出版日期:

2015-04-20

文章信息/Info

Title:

Quantitative analysis of plasma mtTFA content in patients with coronary atherosclerosis heart disease and its potential clinical value

作者:

路轶晴¹; 3; 陈书影²; 郭文怡

(1.西安市第一医院心血管内科，陕西 西安 710002；
2.解放军第323医院心血管内科，陕西 西安 710054；
3.第四军医大学西京医院心血管内科，陕西 西安 710032)

Author(s):

LU Yi-qing¹; 3;  CHEN Shu-ying²;  GUO Wen-yi³

(1.Department of Cardiology, Xi’an First Hospital, Xi’an 710002, Shaanxi, China；
2.Deptment of Cardiology, PLA 323 Hospital, Xi’an 710054, Shaanxi, China；
3.Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)

关键词:

血浆线粒体转录因子A; 冠状动脉疾病; 酶联免疫吸附法

Keywords:

plasma mtTFA;  coronary disease;  enzyme-linked immunosorbent assay

分类号:

R541.4

DOI:

-

文献标识码:

A

摘要:

目的 定量检测冠心病患者血浆线粒体转录因子A（mtTFA）的含量并探讨其与冠心病病变程度的关系及其临床应用价值。方法 收集187例冠心病和190例健康志愿者的外周血样本，应用ELISA检测各组血浆mtTFA的表达水平，比较分析血浆mtTFA水平与冠心病的临床相关性，并绘制研究对象工作特征（ROC）曲线。结果 冠心病患者血浆mtTFA含量显著高于对照组（P<0.01）。在冠心病各亚组中，不稳定型心绞痛组血浆mtTFA含量高于稳定型心绞痛组，而急性心肌梗死组mtTFA含量分别高于稳定型心绞痛和不稳定型心绞痛组（P<0.01）。血浆mtTFA含量与冠心病患者TG、LDL-C、AIP以及病变程度显著正相关（均P<0.05）。用血浆mtTFA含量绘制ROC曲线，曲线下面积为0.790（95%可信区间为0.721-0.849）。以血浆mtTFA含量≥2.26 μg/L为截断点，用血浆mtTFA含量诊断冠心病的敏感度为72.2%，特异度为79.2%。结论 血浆mtTFA含量与冠心病病变程度显著相关，有一定的临床诊断价值，可作为冠心病发病监测及早期诊断的检测指标。

Abstract:

AIM To quantitatively examine plasma mtTFA content of patients with coronary atherosclerosis heart disease (CAHD) and to evaluate its application as a risk predictor for CAHD. MEHTODS Blood samples were collected from 187 CAHD patients, including 94 cases with stable angina pectoris (SAP), 56 with unstable angina pectoris (UAP) and 37 with acute myocardial infarction (AMI), and 190 healthy controls. Plasma mtTFA content in each group was detected by enzyme-linked immunosorbent assay. The clinical association of plasma mtTFA content with CAHD was analyzed and receiver operating characteristic (ROC) curve was performed to predict the risk of CAHD by plasma mtTFA content. RESULTS CAHD patients exhibited statistically significantly higher plasma mtTFA content (median:AMI, 3.17 μg/L; UAP, 2.32 μg/L; SAP, 1.92 μg/L) than matched controls (median:1.79 μg/L; P<0.01). Plasma mtTFA content was positively associated with TG (P=0.018), LDL-C (P=0.015) and AIP (P=0.009).Compared with individuals with lower mtTFA content, individuals with higher mtTFA content had a significantly increased risk of CAHD (adjusted OR,2.84; 95%CI,1.21-3.96) when healthy controls were used as the reference group. A significant dose-response relation was observed between CAHD risk and higher mtTFA content (P for trend <0.001). In addition, area under the ROC curve performed by the plasma mtTFA from all participants was 0.790 (95%CI: 0.721-0.849). With a cutoff value of 2.26 μg/L, overall sensitivity was 72.2% and specificity was 79.2%. CONCLUSION Plasma mtTFA content is positively related to the risk of CAHD. Plasma mtTFA content could be used to monitor the occurrence of CAHD and may be a potential marker for early diagnosis of CAHD.

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备注/Memo

备注/Memo:

收稿日期：2014-07-26.
通讯作者：郭文怡，主任医师，主要从事冠心病临床与基础研究 Email:Guowenyi@tom.com
作者简介：路轶晴，住院医师，硕士生 Email：88295959@qq.com

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更新日期/Last Update: 2015-04-22