大鼠心脏缺血/再灌注后心肌中GLP-1R表达的变化
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2015年第5期
- 页码:
- 528-530,539
- 栏目:
- 基础研究
- 出版日期:
- 2015-05-05
文章信息/Info
- Title:
- Presence and time course of GLP-1R expression after myocardial ischemia/reperfusion injury in heart
- Author(s):
- PAN Xie-tian; ZHANG Ming-ming; SUN Dong-dong; LI Cong-ye; GAO Hao-kao; WANG Hai-chang
- (Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, Shaanxi, China)
- 关键词:
- 心脏病; 缺血性; 缺血/再灌注; 胰高血糖素样肽-1受体
- 分类号:
- R542.22
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 观察大鼠心脏缺血/再灌注(I/R)不同时间点心肌中胰高血糖素样肽-1受体（glucagon-like peptide 1 receptor，GLP-1R）表达的变化。方法 将雄性SD大鼠左前降支动脉结扎30 min建立心肌梗死(MI)模型，然后松开血管进行再灌注。心电向量示波图和心脏彩超均证实MI模型成功建立。在再灌注的不同时间点，用免疫组化染色法及Western blot半定量检测GLP-1R受体表达水平的变化。结果 心电向量示波图证实MI发生。模型组再灌注后1 d心脏超声示，大鼠射血分数〔(70.4±0.5)%〕较Sham组〔(87.4±1.0)% 〕明显下降(P<0.01)。与Sham组相比，Western blot测定显示，GLP-1R的表达在I/R后8 h升高并达到峰值(P<0.01)，再灌注后1 d和3 d开始下降，但仍高于Sham组(P<0.05,P<0.01)，再灌注7 d后恢复正常水平，与Sham组无差异。免疫组化染色法的检测结果变化与Western blot法的检测结果规律相同。结论 大鼠心脏I/R损伤可激活心肌中GLP-1R先上调后恢复正常水平的表达变化。
- Abstract:
- AIM To observe the presence and time course of GLP-1R expression after myocardial ischemia/reperfusion (MI/R) injury in rats. METHODS Male Sprague Dawley rats underwent a 30-min transient left coronary artery occlusion followed by reperfusion. Myocardial infarction was confirmed by electrocardioscope and cardiac ultrasound. Immunohistochemical staining and Western blot of GLP-1R were performed to observe GLP-1R expression in rats at different time points. RESULTS Decrease of cardiac ejection fraction was observed by cardiac ultrasound. GLP-1R expression by Western blot analysis reached a peak at the time point after 8 h reperfusion, significantly higher than in the control group (P<0.01) and remained at a high level at day 1 and day 3 compared with those in sham-operated rats (P<0.05, P<0.01). At 1 week after MI/R, GLP-1R expression showed no significant difference compared with that in healthy myocardium. Immunohistochemical staining showed the same trend of GLP-1R expression by Western blot analysis in the myocardial infarcted/ischemic area. CONCLUSION Myocardial ischemia/reperfusion activates the change of GLP-1R expression first by increase and then recovery to normal.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2014-10-21.
基金项目：国家自然科学基金项目资助（81100234）
通讯作者：王海昌,主任医师,主要从事心律失常及冠心病研究Email：wanghc@fmmu.edu.cn
共同通讯作者：高好考，主治医师，主要从事心血管分子成像研究 Email:hk_gao@163.com
作者简介：潘谢添，硕士生 Email:xietian-111@163.com
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