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|本期目录/Table of Contents|

血管钠肽对大鼠腹主动脉平滑肌细胞ATP敏感钾通道的作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2015年第6期
页码:
650-652
栏目:
基础研究
出版日期:
2015-07-15

文章信息/Info

Title:
Effects of vasonatrin peptide on ATP-sensitive potassium channel of abdominal aortic smooth muscle cells in rats
作者:
陈 睿1; 2; 刘晓东3; 张 婧2; 铁 茹4; 朱妙章4; 师建国1
（第四军医大学：1.基础部病理学教研室，2.西京医院预防保健科，3.口腔医院解剖生理教研室，4.基础部生理学教研室，陕西 西安 710032）
Author(s):
CHEN Rui1; 2;  LIU Xiao-dong3;  ZHANG Jing2;  TIE Ru4;  ZHU Miao-Zhang3;  SHI Jian-guo1
(1.Depatrment of Pathology, School of Basic Medical Sciences, 2.Department of Prevention and Health, Xijing Hospital, 3.Department of Dental Anatomy and Physiology, School of Stomatology, 4.Depatrment of Physiology, School of Basic Medical Sciences, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
血管钠肽; ATP敏感钾通道
Keywords:
vasonatrin peptide;  ATP-sensitive potassium channel
分类号:
TQ464.7
DOI:
-
文献标识码:
A
摘要:
目的 观察血管钠肽(VNP)对大鼠腹主动脉的舒张作用及对ATP敏感性钾通道（ATP-sensitive potassium channel,KATP）的作用。方法 采用离体血管灌流方法，测定VNP对血管环张力的影响。采用膜片钳方法观察VNP对KATP通道的影响。结果 VNP对大鼠的腹主动脉具有浓度依赖性舒张作用，该作用无内皮依赖性：VNP对内皮完整的腹主动脉最大舒张率(Emax)为（81±8）%，对内皮缺失腹主动脉Emax为（74±6）%。在浴槽内预先孵育优降糖(1×10-6 mol/L)可降低血管对VNP的舒张效应。VNP可增强腹主动脉血管平滑肌细胞KATP通道电流： VNP（1×10-6 M）增强KATP通道Emax为（112±24）%，优降糖(1×10-6 mol/L)可消除这一作用。结论 VNP有舒张血管作用，这一作用与增强KATP通道电流有关。
Abstract:
AIM To observe the effects of vasonatrin peptide (VNP) on rat abdominal aorta and on ATP-sensitive potassium channels (ATP-sensitive potassium channel, KATP). METHODS Isolated vascular perfusion method was used to determine the effects of VNP on vascular tension and patch clamp was used to observe the effects of VNP on KATP channel. RESULTS VNP evoked concentration-dependent and endothelium-independent relaxations on isolated abdominal aortic artery. Emax of VNP on endothelium intact abdominal aortic artery was (81±8)% and Emax on endothelial denuded abdominal aortic artery was (74±6)%. The relaxations induced by VNP on blood vessels were attenuated by preincubation in organ chambers with glibenclamide (1×10-6 mol/L). VNP enhanced KATP channel currents of abdominal aortic vascular smooth muscle cell. Emax of VNP (1×10-6 M) reinforced KATP channel currents was (112±24)%, and the effects were eliminated by glibenclamide (1×10-6 mol/L). CONCLUSION VNP is an effective vasorelaxing peptide whose action is caused by enhancing currents of ATP-sensitive potassium channel.

参考文献/References

[1]Nishimura K,Nishimura T,Onishi K,et al.Changes in plasma levels of B-type natriuretic peptide with acute exacerbations of chronic obstructive pulmonary disease[J].Int J Chron Obstruct Pulmon Dis,2014,9:155-162.
[2]Tinker A1,Aziz Q,Thomas A.The role of ATP-sensitive potassium channels in cellular function and protection in the cardiovascular system[J].Br J Pharmacol,2014,171(1):12-23.
[3]Teramoto N,Zhu HL,Shibata A,et al.ATP-sensitive K+ channels in pig urethral smooth muscle cells are heteromultimers of Kir6.1 and Kir6.2[J].Am J Physiol Renal Physiol,2009,296(1):F107-F117.
[4]Zingman LV,Zhu Z,Sierra A,et al.Exercise-induced expression of cardiac ATP-sensitive potassium channels promotes action potential shortening and energy conservation[J].J Mol Cell Cardiol,2011,51(1):72-81.
[5]Aziz Q,Thomas AM,Khambra T,et al.Regulation of the ATP-sensitive potassium channel subunit,Kir6.2, by a Ca2+-dependent protein kinase C[J].J Biol Chem,2012,287(9):6196-6207.

备注/Memo

备注/Memo:
收稿日期：2015-02-09.
通讯作者：师建国，教授，主要从事心脏病理学研究 Email：2473632607@qq.com
作者简介：陈睿，硕士生 Email：lzhchenr@126.com
更新日期/Last Update: 2015-07-23