我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

有氧运动训练对大鼠心肌缺血/再灌注损伤致心肌细胞凋亡的作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2015年第6期
页码:
659-661,665
栏目:
基础研究
出版日期:
2015-07-15

文章信息/Info

Title:
Effects of aerobic exercise training on myocardial apoptosis induced by ischemia-reperfusion injury
作者:
于新彦1; 杨省利2
（1.西安体育学院公共体育教研室，陕西 西安 710068；
2.第四军医大学西京医院心血管内科，陕西 西安 710032）
Author(s):
YU Xin-yan1;  YANG Sheng-li2
(Department of Public Physical Education, Xi’an Sport University, Xi’an 710068, Shaanxi, China；
2.Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
缺血/再灌注损伤; 有氧运动训练; 凋亡; 磷脂酸肌醇3激酶蛋白激酶B
Keywords:
Ischemia/reperfusion injury;  aerobic exercise training;  apoptosis;  PI3K/Akt
分类号:
R87
DOI:
-
文献标识码:
A
摘要:
目的 应用大鼠在体心脏缺血/再灌注损伤（ischemia/reperfusion injury,I/RI）动物模型，观察缺血/再灌注(I/R)后运动训练（exercise training）对心肌细胞凋亡的作用以及与磷脂酸肌醇3激酶蛋白激酶B（PI3K/Akt）信号通路可能存在的相互作用，探讨运动训练对心脏保护作用的可能机制。方法 60只SD大鼠建模后随机分成3组：假手术组，I/R组，I/RI+运动训练组。LTTC染色检测心肌梗死范围；采用TUNEL荧光标记法检测心肌凋亡；Caspase-3试剂盒检测caspase-3活性；免疫印迹法检测p-Akt水平。结果 与I/R组比较，运动训练可以减少心肌梗死范围（n=9，P<0.01），可以明显抑制心肌细胞凋亡（n＝10，P<0.01），降低caspase-3活性（n=8，P<0.01），有效提高I/R后心肌p-Akt水平（n=7，P<0.01）。结论 运动训练对I/RI的心肌具有保护作用，其可能是通过PI3K/Akt信号通路实现的。
Abstract:
AIM To investigate the effects of aerobic exercise training on myocardium undergoing ischemia and reperfusion injury (I/RI). METHODS Sixty male healthy Sprague Dawley rats were randomized into three groups: A, sham operation group; B, I/RI group, ischemia 30 min, reperfusion 4 h, and C, I/RI+aerobic exercise training group. Myocardial infarct size, apoptotic index of cardiomyocytes, caspase-3 activity and p-Akt levels were detected. RESULTS Aerobic exercise training reduced myocardial infarct size (n=9, P<0.01), inhibited cardiomyocyte apoptosis (n=10, P<0.01), decreased caspase-3 activity (n=8, P<0.01), and significantly increased p-Akt levels (n=7, P<0.01). CONCLUSION Aerobic exercise training plays a protective role on myocardium undergoing ischemia and reperfusion injury, and its protective effect is partially through PI3K/Akt signal pathway.

参考文献/References

[1]Abbate A,Biondi-Zoccai GG,Baldi A. Pathophysiologic role of myocardial apoptosis in post-infarction left ventricular remodeling[J].J Cell Physiol,2002,193(2):145-153.
[2]Gottlieb RA,Engler RL.Apoptosis in myocardial ischemia-reperfusion[J].Ann N Y Acad Sci,1999,874:412-426.
[3]Tomei LD,Umansky SR.Apoptosis and the heart:a brief review[J].Ann N Y Acad Sci,2001,946:160-168.
[4]Demirel HA,Powers SK,Zergeroglu MA,et al. Short-term exercise improves myocardial tolerance to tn vivo ischemia-reperfusion in the rat[J].J Appl Physiol,2001,91(5):2205-2212.
[5]Venditti P,Masullo P,Di Meo S,et al.Effects of prolonged aerobic exercise on myocardial responses to ischemia reperfusion in the rat[J].Exp Physiol,2001,86(3):341-348.
[6]Lennon SL,Quindry JC,French JP,et al.Exercise and myocardial tolerance to ischaemia-reperfusion[J].Acta Physiol Scand,2004,182(2):161-169.
[7]Siu PM,Bryner RW,Martyn JK,et al.Apoptotic adaptations from exercise training in skeletal and cardiac muscles[J].FASEB J,18(10):l150-l152.
[8]朱红军,江钟立.运动与氧化应激的预适应[J].中国康复医学杂志,2003,18(2):122-124.
[9]Lajoie C,Calderone A,Beliveau L.Exercise training enhanced the expression of myocardial proteins related to cell protection in spontaneously hypertensive[J].Pflugers Arch,2004,49(1):26-32.
[10]姚红华,陈银海.强化训练对脑卒中偏瘫患者上肢运动功能的影响[J].中国康复医学杂志,2007,22(2):142-143.

备注/Memo

备注/Memo:
收稿日期：2015-06-02.
通讯作者：杨省利，副主任护师，主要从事心血管疾病研究 Email:xzyangxl@fmma.edu.cn
作者简介：于新彦，副教授，硕士 Email：rtxy2004@163.com
更新日期/Last Update: 2015-07-23