糖尿病调控大鼠心肌细胞肌浆网钙离子通道表达的研究
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2016年第2期
- 页码:
- 136-138,144
- 栏目:
- 基础研究
- 出版日期:
- 2015-11-25
文章信息/Info
- Title:
- Effects of diabetes on Ca2+ channel of sarcoplasmic reticulum in rat cardiomyocytes
- Author(s):
- ZHAO Shu-mei; ZHANG Qian; WU Yong-quan; GUO Chun-yan
- (Cardiovascular Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China)
- Keywords:
- diabetes; Ca2+ signal; Ca2+ channel; regulatory protein
- 分类号:
- R473.2
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 评价糖尿病对大鼠心室肌细胞肌浆网钙离子转运通道及其调控蛋白表达的影响。方法 以注射链脲佐菌素的方法制备1型糖尿病大鼠模型；动物分成4组(n=6)：对照组、糖尿病4周组、糖尿病8周组和糖尿病12周组。分别以RT-PCR和Western blot的方法测定心室肌细胞肌浆网上主要钙转运通道及其调控蛋白的mRNA和蛋白的表达变化。结果 糖尿病程第4周，心肌细胞肌浆网上主要钙转运通道（雷诺定受体和Serca2ATPase）表达开始显著下降，并持续下降至病程12周。同时，调控蛋白FKBP12.6表达第8周显著下降、phospholamban表达同步上升。结论 随着病程的进展，糖尿病大鼠心肌细胞肌浆网钙离子转运通道及其调控蛋白的表达呈逐步和显著的下降趋势。
- Abstract:
- AIM To evaluate the effects of diabetes on the expressions of Ca2+ channels and regulatory proteins on sarcoplasmic reticulum (SR) in rat cardiomyocytes. METHODS Sprague Dawley rats were divided into four groups (n=6, each group): group A (control group) and streptozotocin-induced diabetic groups: group B (4-week group), group C (8-week group), and group D (12-week group). Expressions of Ca2+ channels and their regulatory proteins were assessed by RT-PCR (mRNA) and Western blotting (proteins), respectively. RESULTS With the progression of diabetes, expressions of both ryanodine receptor 2 (RyR2) and sarcoplasmic reticulum Ca2+-2ATPase (Serca) decreased significantly, with the downregulation of FKBP12.6 and upregulation of phospholamban. CONCLUSION With progression of disease, diabetes induces the declines in expressions of Ca2+-associated channels and regulatory proteins on SR in cardiomyocytes of rats, gradually and significantly.
参考文献/References
[1]Zhao SM,Li HW,Guo CY,et al.Cardiac fibrosis in diabetic rats: regulation and mechanism of activation of the PPARgamma signal pathway[J].Chin J Physiol,2010,53(4):262-267.
[2]Pereira L,Ruiz-Hurtado G,Rueda A.Calcium signaling in diabetic cardiomyocytes[J].Cell Calcium,2014,56(5):372-380.
[3]Lamberts RR,Lingam SJ,Wang HY,et al.Impaired relaxation despite upregulated calcium-handling protein atrial myocardium from type 2 diabetic patients with preserved ejection fraction[J].Cardiovasc Diabetol,2014,13:72.
[4]Lacombe VA,Viatchenko-Karpinski S,Terentyev D,et al.Mechanisms of impaired calcium handling underlying subclinical diastolic dysfunction in diabetes[J].Am J Physiol Regul Integr Comp Physiol,2007,293(5):R1787-R1797.
[5]Zhao SM,Wang YL,Guo CY,et al.Progressive decay of Ca2+ homeostasis in the development of diabetic cardiomyopathy[J].Cardiovasc Diabetol,2014,13:75.
[6]Marks AR.Ryanodine receptors/calcium release channels in heart failure and sudden cardiac death[J].J Mol Cell Cardiol,2001,33(4):615-624.
[7]Bers DM.Cardiac excitation-contraction coupling[J].Nature,2002,415(6868):198-205.
[8]Mattiazzi A,Mundina-Weilenmann C,Guoxiang C,et al.Role of phospholamban phosphorylation on Thr17 in cardiac physiologi-cal and pathological conditions[J].Cardiovasc Res,2005, 68(3):366-375.
[9]Bhupathy P,Babu GJ,Periasamy M.Sarcolipin and phospholamban as regulators of cardiac sarcoplasmic reticulum Ca2+ ATPase[J].J Mol Cell Cardiol,2007,42(5):903-911.
[10]Galfré E,Pitt SJ,Venturi E,et al.FKBP12 activates the cardiac ryanodine receptor Ca2+-release channel and is antagonised by FKBP12.6[J].PLoS One,2012,7(2):e31956.
备注/Memo
- 备注/Memo:
- 收稿日期：2014-12-26.
通讯作者：郭春艳，副主任医师，主要从事心血管内科基础与临床研究 Email：Cardiovas@hotmail.com
作者简介：赵树梅，副主任医师，博士 Email：yd678182@163.com
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