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普罗帕酮对兔心室肌细胞INa-T的张力性阻滞和使用依赖性阻滞

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2016年第2期
页码:
153-156
栏目:
基础研究
出版日期:
2015-11-25

文章信息/Info

Title:
Tonic block and use-dependent block of transient sodium current by propafenone in rabbit ventricular myocytes
作者:
李 晶1; 赵籥陶1; 周 刚1; 郑霄云2; 杨 震3
(1.北京医院老年科，北京 100730；
2.中日友好医院高干科，北京 100029；
3.宁夏医科大学总医院心脏中心内科，宁夏 银川 750004）
Author(s):
LI Jing1;  ZHAO Yue-tao1;  ZHOU Gang1;  ZHENG Xiao-yun2;  YANG Zhen3
(1.Department of Geriatrics, Beijing Hospital, Beijing 100730, China；
2.Department of Geriatrics, China-Japan Friendship Hospital, Beijing 100029, China；
3.Department of Cardiology, General Hospital, Ningxia Medical University, Yinchuan 750004, Ningxia, China)
关键词:
普罗帕酮; 瞬时钠电流; 张力性阻滞; 使用依赖性阻滞
Keywords:
propafenone;  transient sodium current;  tonic block;  use-dependent block
分类号:
R331.3
DOI:
-
文献标识码:
A
摘要:
目的 探讨普罗帕酮对兔心室肌细胞瞬时钠电流（INa-T）的张力性阻滞和使用依赖性阻滞作用。方法 采用全细胞膜片钳技术检测兔心室肌细胞INa-T的I-V曲线及不同频率下的峰值电流，并用10 μmol/L普罗帕酮溶液灌流干预。 结果 普罗帕酮灌流后INa-T的I-V曲线明显上移，峰值电流显著下降〔(3001±383) pA vs.(4193±378) pA，P<0.05〕。以0.06 Hz和1Hz频率刺激时，普罗帕酮未显示使用依赖性阻滞作用。当以2 Hz、5 Hz和10 Hz频率刺激时，阻滞率分别为（22±11）%、（38±14）%和（52±17）%，3组间两两比较以及与对照组和普罗帕酮在0.06 Hz和1 Hz时的阻滞率的差异有显著统计学意义(P<0.05)。结论 普罗帕酮对INa-T不但有张力性阻滞作用，也有显著的使用依赖性阻滞作用。
Abstract:
AIM To study the tonic block and use-dependent block of transient sodium current (INa-T) by propafenone in rabbit ventricular myocytes. METHODS INa-T was measured and I-V curves of INa-T obtained using whole cell patch clamp before and after the perfusion of propafenone (10 μmol/L). RESULTS After the perfusion of propafenone, the I-V curve of INa-T shifted upward and the peak current decreased ［(3001±383) pA vs.(4193±378) pA, P<0.05］. After perfusion of propafenone, when stimulated at 0.06 Hz and 1 Hz, no significant use-dependent block was observed, whereas with stimulation at 2, 5 and 10 Hz, significant use-dependent block of INa-T was found with inhibition fractions of (22±11)%, (38±14)% and (52±17)%, respectively, with statistically significant difference when compared between any two of the three groups and compared with the inhibition fractions at 0.06 Hz and 1 Hz (P<0.05). CONCLUSION Propafenone produces significant tonic block and use-dependent block of INa-T.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2015-01-26.
通讯作者：杨震，副主任医师，主要从事心脏电生理研究 Email：yangzhen080@163.com
作者简介：李晶，副主任医师，硕士 Email:jing-li2000@126.com
更新日期/Last Update: 2016-04-25