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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2016年第3期

页码:

263-267

栏目:

基础研究

出版日期:

2016-01-05

文章信息/Info

Title:

Supplemental mechanism of increased reperfusion injury in senescent myocardial ischemia: significant role of necroptosis

作者:

李 晨¹; 陈 迈²; 马 恒¹; 3

（第四军医大学：1.基础医学院病理生理学教研室，2.西京医院心血管内科，3.基础医学院生理学教研室，陕西 西安 710032）

Author(s):

LI Chen¹;  CHEN Mai²;  MA Heng¹; 3

(1.Department of Pathophysiology, 2.Department of Cardiology, Xijing Hospital, 3.Department of Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)

关键词:

衰老; 程序性坏死; 心肌缺血/再灌注

Keywords:

aging;  necroptosis;  myocardial ischemia reperfusion

分类号:

R542.2

DOI:

-

文献标识码:

A

摘要:

目的 探讨程序性坏死（necroptosis）在衰老心肌缺血/再灌注(I/R)损伤中的关键作用。 方法 成年（ 3~4月龄）和老龄（ 22~24月龄）雄性C57BL/6小鼠20只各随机分为对照组与I/R组，建立小鼠急性心肌I/R模型（缺血30 min再灌注4 h）。再灌注结束后，取心肌组织并分别应用蛋白质免疫印迹法（Western blot）和免疫共沉淀法（Co-Immunoprecipitation;Co-IP）检测necroptosis标志蛋白的表达及其修饰变化。结果 与成年心肌相比，necroptosis的标志蛋白RIP1、RIP3在衰老心肌中的表达均升高（P<0.05），necroptosis的调节蛋白去乙酰化酶SIRT2的表达及活性也显著升高（P<0.05），RIP1的去乙酰化水平显著升高（P<0.05）。给予小剂量necroptosis抑制剂Necrostatin-1(Nec-1)处理，可以显著减少衰老心肌I/R梗死面积（P<0.05）。结论 本研究发现在衰老心肌I/R损伤中necroptosis显著增加，表明necroptosis可能在衰老心肌缺血损伤中有重要作用。

Abstract:

AIM Research shows that aging hearts have less tolerance to myocardial ischemia reperfusion (I/R) injury and this leads to the design of investigating the role of necroptosis in senescent myocardial I/R injury. METHODS Male C57BL/6 mice aged 22-24 months were randomized into I/R group and vehicle control group. Adult male (3-4 months) C57BL/6 mice served as control group. The left anterior descending artery was ligated for 30 min followed by 4 h reperfusion to establish the acute myocardial I/R mouse model. Myocardial tissue was obtained after reperfusion. Western blot was used to detect necroptosis-related protein expression. Co-immunoprecipitation was used to detect the modification of the necroptosis-related protein. RESULTS Necroptosis was commonly increased in hearts of aged mice (P<0.05). The expression of necroptosis biomarker, RIP1 and RIP3, is increased (P<0.05). Expression and activity of the regulator protein deacetylase SIRT2 increased as well as the deacetylation of RIP1 (P<0.05). Meanwhile, inhibiting necroptosis by using the inhibitor necrostatin-1 (Nec-1) reduced the infarct size in aged hearts (P<0.05). CONCLUSIONN ecroptosis displays an increased expression in aged myocardial I/R injury, indicating the important role necroptosis plays in age-related myocardial ischemia injury.

参考文献/References

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备注/Memo

备注/Memo:

收稿日期：2015-11-04.
基金项目：国家自然科学基金项目资助（81170108,81470410）;国家优秀青年科学基金项目资助(81322004)
通讯作者：马恒，副教授，主要从事心肌内源性保护机制研究 Email：hengma@fmmu.edu.cn
作者简介：李晨，硕士生 Email：diyisun1115@qq.com

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更新日期/Last Update: 2016-01-07