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|本期目录/Table of Contents|

EMRE对高糖高脂诱导心肌细胞凋亡的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2016年第3期
页码:
285-288
栏目:
基础研究
出版日期:
2016-01-05

文章信息/Info

Title:
Effects of EMRE on apoptosis of cardiomyocyte induced by high glucose and saturated fatty acids
作者:
荆 哲1; 刘峰舟1; 王炜中2; 李 飞1
（第四军医大学：1.西京医院心血管内科，2.学员旅，陕西 西安 710032）
Author(s):
JING Zhe1;  LIU Feng-zhou1;  WANG Wei-zhong2;  LI Fei1
(1.Department of Cardiology, Xijing Hospital, 2.Cadet Brigade, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
EMRE; 高糖高脂; 心肌细胞; 活性氧簇
Keywords:
EMRE;  high glucose and saturated fatty acids;  H9C2;  cardiomyocyte;  ROS
分类号:
R329.2
DOI:
-
文献标识码:
A
摘要:
目的 研究EMRE分子在高糖/高脂（high glucose and high fat,HGHF）培养的大鼠胚胎心肌细胞H9C2中的表达变化及其在HGHF诱导的心肌细胞凋亡中的作用。方法 H9C2细胞培养分为4组：即正常+siCtrl组（培养基中含5 mmol/L葡萄糖、20 mmol/L甘露醇和siCtrl，培养24 h）、正常+si-EMRE组（培养基中含5 mmol/L葡萄糖、20 mmol/L甘露醇和si-EMRE，培养24 h）、HGHF+siCtrl组（培养基中含25 mmol/L葡萄糖、500 μmol/L软脂酸钠和siCtrl，培养24 h）及HGHF+si-EMRE组（培养基中含25 mmol/L葡萄糖、500 μmol/L软脂酸钠和si-EMRE，培养24 h）。用qRT-PCR及Western blot检测EMRE在各组细胞中的表达；用流式细胞术（FCM）分析各组心肌细胞的凋亡；用Western blot检测caspase3与caspase9表达；用荧光探针DCFH-DA检测各组心肌细胞内ROS水平。结果 HGHF诱导心肌细胞EMRE表达升高（mRNA及蛋白水平）；EMRE促进了HGHF诱导的心肌细胞凋亡及ROS的产生；用si-RNA干涉EMRE表达后，由HGHF诱导的细胞凋亡及ROS产生明显减少。结论 EMRE在HGHF诱导的心肌细胞凋亡中发挥重要的促进作用。
Abstract:
AIM To investigate the expression of EMRE and its regulatory role in apoptosis of H9C2 cells induced by high glucose and saturated fatty acids (HGHF). METHODSH9C2 cells were divided into four groups: control+siCtrl group (5 mmol/L glucose, 20 mmol/L mannitol and siCtrl, incubated 24 h), control+si-EMRE group (5 mmol/L glucose, 20 mmol/L mannitol and si-EMRE, incubated 24 h), HGHF+siCtrl group (25 mmol/L glucose, 500 μmol/L palmitate sodium and siCtrl, incubated 24 h), and HGHF+si-EMRE group (25 mmol/L glucose, 500 μmol/L palmitate sodium and si-EMRE, incubated 24 h). The mRNA and protein expression levels of EMRE were detected by qRT-PCR and Western blotting. Cell apoptosis was analyzed using flow cytometry, the expressions of caspase3 and caspase9 were detected by Western blotting and the intracellular ROS levels were assayed using fluorescence probe DCFH-DA. RESULTSHGHF induced up-regulation of EMRE in H9C2 cells both at mRNA and protein levels. EMRE was critical for HGHF-induced cell apoptosis and ROS production. Knocking down of EMRE using si-RNA significantly inhibited HGHF-induced cell apoptosis and ROS production. CONCLUSIONEMRE is critical for HGHF-induced cell apoptosis.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2015-05-11.
通讯作者：李飞，副主任医师，主要从事糖尿病心肌病与心血管保护研究 Email：Lifeil@fmmu.edu.cn
作者简介：荆哲，硕士生 Email：35082774@qq.com
更新日期/Last Update: 2016-01-07