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|本期目录/Table of Contents|

整合素信号转导通路参与模拟失重引起的血管适应性变化

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2016年第4期
页码:
396-400
栏目:
基础研究
出版日期:
2016-04-01

文章信息/Info

Title:
Integrin signaling pathway is involved in adaptive changes of blood vessels induced by simulated weightlessness
作者:
茹凝玉; 白云刚; 余志斌; 程九华
(第四军医大学航空航天医学教育部重点实验室、航空航天生理学教研室，陕西 西安710032)
Author(s):
RU Ning-yu;  BAI Yun-gang;  YU Zhi-bin;  CHENG Jiu-hua
(Key Laboratory of Aerospace Medicine, Ministry of Education & Department of Aerospace Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
模拟失重; 间断性人工重力; 血管重构; 整合素; 血管灌流培养
Keywords:
simulated microgravity;  intermittent artificial gravity;  vascular remodeling;  integrin;  artery organoid culture
分类号:
R852.22
DOI:
-
文献标识码:
A
摘要:
目的 本研究将模拟失重大鼠整体实验与离体血管灌流培养实验相结合，重点观察在不同跨壁压条件下整合素(integrin)及其关联的信号转导分子的表达、激活、募集等变化规律。方法 整体实验：大鼠尾部悬吊法模拟失重4周（SUS组），并设同步对照组（CON组）。观察模拟失重对大鼠Integrin αV、Integrin β3、Src、ERK和p-ERK蛋白表达的影响。血管培养实验：建立可以控制流量、压力的血管培养系统。结果 悬吊组与对照组相比，颈总动脉Integrin αV、Src和p-ERK蛋白表达显著上调(P<0.05)，而Integrin β3和ERK蛋白表达无显著性差异。在血管培养实验中，与常压组(80 mmHg)相比，高压(150 mmHg)灌流可引起Integrin αV、Integrin β3、Src、ERK和p-ERK升高(P<0.05)，但在3 d的高压灌流期间，如每天1 h使灌流压降为80 mmHg，则上述改变即可被部分改善，即Integrin αV、Integrin β3和Src蛋白表达恢复至接近常压(80 mmHg)水平。结论 跨壁压分布变化是失重引起动脉区域性重构的始动原因；但每日只要短时间使其恢复常压，血管重构及整合素下游分子的变化即可被部分防止。
Abstract:
AIM To observe expression, activation and recruitment of integrin changes and associated signal transduction molecules under different transmural pressures by combining simulated weightlessness treatment and vascular perfusion culture. METHODSWhole animal level: Effects of simulated weightlessness on the expressions of integrin αV, integrin β3, Src, ERK and p-ERK were observed. Blood vessel level: The pressure vessel culture system that could control the flow rate and pressure was established. RESULTSWestern blot analysis revealed that an increased expression of integrin αV, Src and p-ERK was observed by tail suspension. No significant difference in integrin β3 and ERK protein expression was detected. High pressure culture of 150 mmHg induced significant protein expression of integrin aV, integrin β3, Src, ERK and p-ERK in carotid artery. Daily 1-h normal pressure recovery of 80 mmHg partly restored the changes of integrin aV, integrin β3 and Src expression. CONCLUSIONRedistribution of transmural pressures may be the primary factor that initiates region-specific remodeling of arteries during microgravity. Daily 1-h normal pressure recovery partly prevents vascular remodeling and changes of integrin downstream molecules.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2015-07-27.
基金项目：国家自然科学基金项目资助(31070839，30470649)
通讯作者：程九华，高级实验师，主要从事心血管生理机制研究 Email：chengjh@fmmu.edu.cn 共同
通讯作者：余志斌，教授，主要从事应用心血管生理学研究 Email：yuzhib@fmmu.edu.cn
作者简介：茹凝玉，助教 Email：runingyu18@126.com
更新日期/Last Update: 2016-04-01