减阻剂减轻大鼠离体心脏缺血/再灌注损伤的作用(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2017年第2期
- 页码:
- 144-148
- 栏目:
- 基础研究
- 出版日期:
- 2016-11-25
文章信息/Info
- Title:
- Protective effects of drag-reducing polymers against ischemia/reperfusion injury in isolated rat heart
- Author(s):
- WU Yong; HU Feng; WANG Ya-li; ZHAO Pei; DU Lin; GONG Kai-zheng; ZHANG Zhen-gang
- (Department of Cardiology, First People’s Hospital of Yangzhou & Second Clinical Medical School, Yangzhou University, Yangzhou 225002, Jiangsu, China)
- 分类号:
- R542.2
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 应用减阻剂聚氧化乙烯（polyethylene oxide,PEO）减轻大鼠离体心脏缺血/再灌注（I/R）损伤的作用。方法 Wistar大鼠50只随机分为5组，制备Langendorff离体心脏I/R模型，分为对照组、I/R组、缺血-低剂量PEO再灌注组（低剂量组）、缺血-中剂量PEO再灌注组（中剂量组）、缺血-高剂量PEO再灌注组（高剂量组）。通过多导生理记录仪记录灌注心脏的左心室最大收缩压峰值（LVPSP）、左室舒张末压峰值（LVEDP）、左室内压等容相最大上升及下降速率（+dp/dtmax，-dp/dtmax）、心率、冠脉流量。检测冠脉流出液的乳酸脱氢酶（lactate dehydrogenase,LDH）的含量。结果 再灌注30 min及60 min后，中剂量及高剂量的PEO对I/R心肌的LVPSP、LVEDP、+dp/dtmax、-dp/dtmax均有明显改善作用（P<0.05）。中剂量及高剂量PEO在维持离体心脏的心率方面优于低剂量组和I/R组（P<0.05），冠脉流出液的容积增加（P<0.05）。应用中剂量及高剂量的PEO灌注后，冠脉流出液中LDH含量明显下降（P<0.05）。结论 PEO作为减阻剂，减低Langendorff离体心脏I/R系统的流体阻力，改善离体心脏的收缩及舒张功能，增加冠脉流出液量，减少心肌细胞损伤，抑制心肌细胞凋亡，对心肌I/R损伤的治疗提供新的研究思路。
- Abstract:
- AIM To investigate the protective effects of drag-reducing polymers against ischemia/reperfusion injury in isolated rat heart. METHODS Fifty Wistar rats were randomly divided into five groups: control group, ischemia/reperfusion group (I/R group), low-dose group, middle-dose group and high-dose group. Isolated rat hearts were used to prepare the Langendorff ischemia reperfusion model. Left ventricular peak systolic pressure (LVPSP), left ventricular end diastolic pressure (LVEDP), +dp/dtmax, -dp/dtmax, heart rate and coronary outflow were recorded by multichannel physiological recorder. Levels of lactate dehydrogenase (LDH) in coronary outflow were also detected. RESULTS At the time points of 30 min and 60 min after reperfusion, polyethylene oxide (PEO) was found to have beneficial effects on LVPSP, LVEDP, +dp/dtmax and -dp/dtmax (all P<0.05) in the middle- and high-dose group. Compared with the control group and low-dose group, the middle-dose group and high-dose group were better in maintaining the heart rate (both P<0.05) and increased the coronary outflow (both P<0.05). Levels of LDH in coronary outflow decreased significantly in the middle-dose and high-dose groups (both P<0.05). CONCLUSION As a classic drag-reducing polymer, PEO reduces the fluid resistance of Langendorff ischemia/reperfusion system, improves cardiac systolic and diastolic function, increases coronary outflow and reduces myocardial cell damage and apoptosis, providing a new potential approach for the treatment of myocardial ischemia/reperfusion injury.
参考文献/References
[1]Toms BA.Some observations on the flow of linear polymer solution through straight tubes at large Reynolds numbers[C].North Holland, Amsterdam:Proceedings of the First International Congress on Rheology.1948,135-141.
[2]Cheng M,Zhang S,Dong H,et al.Improving the durability of a drag-reducing nanocoating by enhancing its mechanical stability[J].ACS Appl Mater Interfaces,2015,7(7):4275-4282.
[3]Zhang S,Ouyang X,Li J,et al.Underwater drag-reducing effect of superhydrophobic submarine model[J].Langmuir,2015,31(1):587-593.
[4]Nottebrock B,Grosse S,Schr?der W.Development of a shear stress sensor to analyse the influence of polymers on the turbulent wall shear stress[J].J Phys Condens Matter,2011,23(18):184121.
[5]Virk PS.An elastic sublayer model for drag reduction by dilute solutions of linear macromolecules[J].J Fluid Mech,1971,45:417-440.
[6]Pacella JJ,Kameneva MV,Brands J,et al.Modulation of pre-capillary arteriolar pressure with drag-reducing polymers:a novel method for enhancing microvascular perfusion[J].Microcirculation,2012,19(7):580-585.
[7]胡 锋，杜荣生，查道刚，等.聚氧化乙烯对大鼠腹主动脉血流量及下肢循环阻力的影响[J].南方医科大学学报2010,30(4):884-887.
[8]Hu F,Zha D,Du R,et al.Improvement of the microcirculation in the acute ischemic rat limb during intravenous infusion of drag-reducing polymers[J].Biorheology,2011,48(3-4):149-159.
[9]胡 锋，查道刚，杜荣生，等.减阻剂对正常大鼠后肢骨骼肌微循环灌注的影响[J].中国医学科学院学报,2011,33(2):183-187.
[10]杜荣生，查道刚，周冰洁,等.聚环氧乙烷对大鼠提睾肌微循环红细胞流速的影响研究[J].南方医科大学学报,2010,30(5):960-965.
[11]Chen X,Zha D,Xiu J,et al.A new hydrodynamic approach by infusion of drag-reducing polymers to improve left ventricular function in rats with myocardial infarction[J].Int J Cardiol,2011,147(1):112-117.
[12]刘永国.心肌缺血/再灌注损伤的机制研究进展[J].医学综述,2010,16(21):3267-3269.
[13]Pacella JJ,Kameneva MV,Csikari M,et al.A novel hydrodynamic approach to the treatment of coronary artery disease[J].Eur Heart J,2006,27(19):2362-2369.
[14]Wang Y,Hu F,Mu X,et al.Protective effects of drag-reducing polymers in a rat model of monocrotaline-induced pulmonary hypertension[J].Biorheology,2016,53(1):13-22.
[15]Kameneva MV,Wu Z,Uryash A,et al.Blood soluble drag-reducing polymers prevent lethality from hemorrhagic shock in acute animal experiments[J].Biorheology,2004,41(1):53-64.
[16]Sakai T,Repko BM,Griffith BP,et al.I.V.infusion of a drag-reducing polymer extracted from A loe vera prolonged survival time in a ratmodel of acute myocardial ischemia[J].Br J Anaesth,2007,98 (1): 23-28.
备注/Memo
- 备注/Memo:
- 收稿日期：2016-01-22.
基金项目：国家自然科学基金青年项目资助（81200144）
通讯作者：胡锋，主治医师，主要从事冠心病的基础与临床研究 Email：huf2007@163.com
作者简介：伍勇，主治医师，硕士 Email：154629676@qq.com
常用功能
统计/Statistics
- 摘要浏览/Viewed779
- 全文下载/Downloads648
- 评论/Comments
