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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2017年第2期

页码:

154-157,163

栏目:

基础研究

出版日期:

2016-11-25

文章信息/Info

Title:

Effect of danhong injection on myocardial enzymes and expression of heat shock protein 70 in chronic stress reaction in rats

作者:

王 凯¹; 宮惠琳¹; 叶季鲜²; 邵丽杰²; 谢学建²; 杨 晶²; 3

（1.西安交通大学第一附属医院病理科，陕西 西安 710061；
2.解放军第323医院心血管内科，陕西 西安 710054；
3.西安医学院研究生院，陕西 西安 710021）

Author(s):

WANG Kai¹;  GONG Hui-lin¹;  YE Ji-xian²;  SHAO Li-jie²;  XIE Xue-jian²;  YANG Jing²; 3

(1.Department of Pathology, First Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China；
2.Department of Cardiology, PLA 323 Hospital, Xi’an 710054, Shaanxi, China；
3.Graduate School, Xi’an Medical University, Xi’an 710021, Shaanxi, China)

关键词:

丹红注射液; 慢性应激; 心肌酶;  热休克蛋白70

Keywords:

danhong injection;  chronic stress;  myocardial enzyme;  heat shock protein 70

分类号:

R544

DOI:

-

文献标识码:

A

摘要:

目的 通过心肌酶和热休克蛋白(HSP)70的表达来观察慢性应激反应中丹红注射液对大鼠心肌的保护作用。方法 将健康成年SD大鼠55只随机分为空白对照组、丹红对照组、慢性应激组和慢性应激丹红组。空白对照组不进行慢性应激处理，也不注射丹红注射液；丹红对照组不进行慢性应激处理，仅每日经腹腔注射丹红注射液10 ml/kg；慢性应激组按照慢性应激反应模型进行处理；慢性应激丹红组在按照慢性应激反应模型进行处理的同时每日经腹腔注射丹红注射液10 ml/kg。待慢性应激持续到设定时间时（3周），剖杀4组大鼠，① 观察各组大鼠心肌酶的变化。②同时采用免疫组织化学法检测各组大鼠心肌组织中HSP70的表达情况。结果 ①与空白对照组比较，丹红对照组、慢性应激组和慢性应激丹红组大鼠乳酸脱氢酶（LDH）和肌酸激酶同工酶 （CK-MB）显著升高（P<0.01）。慢性应激组LDH和CK-MB较丹红对照组显著升高（P<0.05），慢性应激丹红组LDH和CK-MB较慢性应激组显著降低（P<0.05）；而丹红对照组和慢性应激丹红组之间无显著性差异。②丹红对照组、慢性应激组和慢性应激丹红组心肌组织HSP70表达均强于空白对照组。丹红对照组和慢性应激组之间HSP70表达无显著性差异。慢性应激丹红组心肌组织HSP70表达强于丹红对照组或慢性应激组。结论 丹红注射液的使用可有效抑制慢性应激大鼠LDH、CK-MB的升高，还可增高慢性应激大鼠心肌组织中HSP70的表达。

Abstract:

AIM To investigate the myocardial protective effect of danhong injection in chronic stress reaction of rats by observing myocardial enzyme and expression of heat shock protein 70 (HSP70). METHODS Fifty-five healthy adult Sprague Dawley rats were randomly divided into blank control group, danhong injection control group, chronic stress group, and chronic stress danhong injection group. The blank control group was treated with neither chronic stress nor danhong injection. The danhong injection control group was treated with intraperitoneal injection of danhong (daily 10 ml/kg) but not the chronic stress group. The chronic stress group was treated according to the chronic stress model processing and the chronic stress danhong injection group was treated in accordance with the treatment of chronic stress reaction model and intraperitoneal injection of danhong injection (daily, 10 ml/kg). After 3-week chronic stress, rats in the four groups were killed, changes of myocardial enzyme were observed, and expression of HSP70 in myocardial tissue was detected using immunohistochemical method. RESULTS Compared with those in the blank control group, lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) in the danhong injection control group, chronic stress group, and chronic stress danhong injection group significantly increased (P<0.01). LDH and CK-MB in the chronic stress group increased significantly compared with those in the danhong injection control group (P<0.05) and LDH and CK-MB in the chronic stress danhong injection group decreased significantly compared with those in the chronic stress group (P<0.05). But no significant difference was found between the danhong injection control group and the chronic stress danhong injection group. Expression of HSP70 of myocardial tissue in the danhong injection control group, chronic stress group, and the chronic stress danhong injection group were stronger than that in the blank control group. There was no significant difference between the danhong injection control group and the chronic stress group. However, the expression of HSP70 in the chronic stress danhong injection group was stronger than that in the danhong injection control group and the chronic stress group. CONCLUSION Danhong injection effectively inhibited the increase of LDH and CK-MB in chronic stress rats and increased the expression of HSP70 in myocardial tissue of chronic stress rats.

参考文献/References

[1]Selye H.A syndrome produced by diverse nocuous agents.1936.[J].J Neuropsychiatry Clin Neurosci,1998,10(2):230-231.
[2]Liu X,Iwanaga K,Koda S.Circulatory and central nervous system responses to different types of mental stress[J].Ind Health,2011,49(3):265-273.
[3]吴 萍.丹红注射液治疗心血管疾病的研究进展[J].中国医药指南,2014,12(6):41-42.
[4]朱宇章，彭 淼，丁宝坤，等.慢性应激对大鼠外显行为及海马亚区脑源性神经营养因子表达差异性的影响[J].中国行为医学科学,2004,13(5):481-483.
[5]Imumorin IG,Dong Y,Zhu H,et al.A gene-environment interaction model of stress-induced hypertension[J].Cardiovasc Toxicol,2005,5(2):109-132.
[6]Choi NG,Kim J,Marti CN,et al.Late-life depression and cardiovascular disease burden: examination of reciprocal relationship[J]. Am J Geriatr Psychiatry,2014,22(12):1522-1529.
[7]李熹娟，詹 皓，辛益妹，等.急慢性正加速度应激条件下大鼠血清心肌酶谱的变化特征[J].现代中西医结合杂志,2012,21(6):596-597.
[8]徐启明，王 艳.应激预处理对应激大鼠心肌的影响[J].中国医药指南,2012,10(20):452-453.
[9]Jones Q,Voegeli TS,Li G,et al.Heat shock proteins protect against ischemia and inflammation through multiple mechanisms[J].Inflamm Allergy Drug Targets,2011,10(4):247-259.
[10]吕春华，郭威早，佟 倩.寒冷应激对心肌细胞HSP70的影响[J].中国老年学杂志,2013,33(18):4509-4511.
[11]王光慧，金玉姬，董传兴，等.热应激对心血管系统的影响[J].吉林医药学院学报,2013,34(2):135-138.
[12]杲修杰，王新兴，弓景波，等.血浆HSP70可作为军事应激负荷的重要生物标志物的研究[J].军事医学,2014,38(3):166-168.
[13]Huang F,Huang JP,Pan JY,et al.Intracellular heat shock protein 70:a possible therapeutic target for preventing postoperative atrial fibrillation[J].Pharmazie,2012,67(9):747-755.
[14]徐建平，张 俐，郑 忠，等.人参皂甙改善Hsp70、Bax及EAAS表达在脊髓缺血再灌注损伤中作用的实验研究[J].生物骨科材料与临床研究,2014,11(6):6-11.
[15]邱宇安，黄绍烈，陈火国，等.黄芪含药血清对血管内皮细胞的保护作用及机制[J].中国当代医药,2015,22(30):108-110.
[16]Chen H,Adam A,Cheng Y,et al.Localization and expression of heat shock protein 70 with rat myocardial cell damage induced by heat stress in vitro and in vivo[J].Mol Med Rep,2015,11(3):2276-2284.

备注/Memo

备注/Memo:

收稿日期：2016-05-04.
基金项目：兰州军区医药卫生科技攻关项目资助（CLZ13JB19）；解放军第323医院重大培育课题资助（2012323A02）
通讯作者：杨晶，副主任医师，主要从事心血管内科临床及离子通道基础研究 Email：yangjing323@163.com
作者简介：王凯，助理研究员，硕士 Email：gbwangkai@163.com

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更新日期/Last Update: 2016-10-11