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|本期目录/Table of Contents|

有氧运动对CSD引起的大鼠IR的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2017年第6期
页码:
652-658
栏目:
基础研究
出版日期:
2017-06-01

文章信息/Info

Title:
Effect of aerobic exercise on insulin resistance induced by chronic sleep deprivation in rats
作者:
顾兰馨1王志法23殷 强2王云雅4
(1.西安市高新第一中学,陕西 西安 710075;
第四军医大学:2.西京医院心脏外科,3.生理学教研室,4.西京医院核医学科,陕西 西安 710032)
Author(s):
GU Lan-xin1 WANG Zhi-fa23 YIN Qiang2 WANG Yun-ya4
(1.Xi’an Gaoxin International High School, Xi’an 710075, Shaanxi, China;
2.Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China;
3.Department of Physiology, School of Basic Medical Sciences, Fourth Military Medical University, Xi’an 710032, Shaanxi, China;
4.Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
慢性睡眠剥夺胰岛素抵抗有氧运动炎症因子大鼠
Keywords:
Chronic sleep deprivation insulin resistance aerobic exercise inflammatory cytokines rat
分类号:
R359.2
DOI:
-
文献标识码:
A
摘要:
目的 观察有氧运动对慢性睡眠剥夺(CSD)引发胰岛素抵抗(IR)的影响,并明确其可能机制。方法 采用改良多平台水环境睡眠剥夺法(MMPM)对大鼠进行为期8周的睡眠剥夺建立CSD模型。雄性Sprague-Dawley大鼠30只,随机分为5组:对照组、睡眠剥夺组、睡眠剥夺+低强度运动组、睡眠剥夺+中强度运动组、睡眠剥夺+高强度运动组。通过测定空腹血清胰岛素、空腹血糖、胰岛素抵抗指数(homeostasis model assessment for insulin resistance,HOMA-IR)、心肌葡萄糖摄取、腹腔糖耐量和空腹胰岛素敏感性等手段观察有氧运动对CSD引发大鼠IR的影响;采用彩色多普勒超声诊断仪检测短轴缩短率(Fractional Shortening,FS)及左心室射血分数(Left Ventricular Ejection Fraction, LVEF);采用放射免疫法测量血清中肿瘤坏死因子(tumor necrosis factor,TNF)-α、白介素(interleukin,IL)-1β、IL-6水平;通过Western blot法检测外周血单核细胞核因子(NF-kappa B,NF-κB)以及抑制蛋白(I-kappa B,I-κB),观察有氧运动对慢性睡眠剥大鼠体内炎症因子水平及心功能的影响。结果 CSD可引起大鼠HOMA-IR、腹腔糖耐量和胰岛素敏感性的明显异常,降低心肌葡萄糖摄取和心脏功能,同时可降低抑炎因子I-κB水平,增加促炎因子NF-κB和血浆炎症因子水平(P<0.05)。低强度运动干预对上述各指标无明显影响;而中强度运动干预则可明显改善大鼠机体IR、心肌IR及心脏射血功能,增加抑炎因子I-κB,降低机体促炎因子NF-κB以及外周各炎症因子的水平(P<0.05);但高强度运动干预则可引起机体IR加重、心肌葡萄糖摄取能力下降及心脏射血分数下降,体内促炎因子NF-κB以及外周各炎症因子水平升高,而抑炎因子I-κB表达水平下降(P<0.05)。结论 适量有氧运动可以通过降低体内炎症反应改善大鼠睡眠剥夺引发的IR及心功能下降。
Abstract:
AIM To explore the effect of aerobic exercise on insulin resistance induced by sleep deprivation and the underlying mechanisms. METHODS A modified multiple platform method was used in a chronic sleep deprivation (CSD) model in rats. Thirty male 8-week-old Sprague-Dawley rats were randomized into a control group, CSD group, CSD with low-intensity aerobic exercise training (CSD+L) group, CSD with moderate intensity exercise training (CSD+M) group and CSD with high-intensity exercise training (CSD+H) group (n=6 per group). The intraperitoneal glucose tolerance test (IPGTT), insulin tolerance test (ITT), positron emission tomography (PET-CT) and insulin resistance index (HOMA-IR) were employed and the levels of IL-1β, IL-6, TNF-α and I-kappa B (I-κB) and NF-kappa B (NF-κB) in peripheral blood mononuclear cells (PBMCs) were assessed. Shortening fraction (FS) and left ventricular ejection fraction (LVEF) were measured by echocardiography after 8-week chronic sleep deprivation in all the groups with or without exercise. RESULTS Compared with those in the control group, CSD decreased HOMA-IR, insulin sensitivity and glucose tolerance as well as peripheral inflammation inhibitory cytokine (Iκ-B), reduced myocardial 18FDG uptake and LVEF but increased proinflammatory cytokine NF-κB. Compared with those in the CSD group, CSD+L resulted in no significant changes in the above indexes. CSD+M markedly lowered CSD induced insulin resistance, decreased the level of peripheral inflammatory cytokines and proinflammatory cytokine NF-κB and increased and myocardial 18FDG uptake and LVEF. CSD+H increased insulin resistance, serum inflammatory cytokines and proinflammatory cytokine NF-κB and decreased peripheral inflammation inhibitory cytokine Iκ-B, myocardial 18FDG uptake and LVEF. CONCLUSION CSD induced significant insulin resistance and systematic inflammation; however, moderate, not low or high, intensity aerobic exercise of aerobic exercise, improved insulin sensibility, at least partially by suppressing the serum inflammatory cytokines in response to CSD, indicating a proper intensity of aerobic exercise could improve LVEF and insulin resistance induced by chronic sleep deprivation.

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备注/Memo

备注/Memo:
收稿日期:2017-03-13.基金项目:国家自然科学基金项目资助(81370352)
通讯作者:王云雅,副主任技师,主要从事心血管疾病干细胞治疗研究 Email:wangyunyaxj@163.com
作者简介:顾兰馨,高中生 Email:2839871189@qq.com 共同第一作者:王志法,住院医师,硕士生 Email:1127101384@qq.com
更新日期/Last Update: 2017-06-06