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|本期目录/Table of Contents|

有氧运动对CSD引起的大鼠IR的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2017年第6期
页码:
652-658
栏目:
基础研究
出版日期:
2017-06-01

文章信息/Info

Title:
Effect of aerobic exercise on insulin resistance induced by chronic sleep deprivation in rats
作者:
顾兰馨1; 王志法2; 3; 殷 强2; 王云雅4
(1.西安市高新第一中学，陕西 西安 710075;
第四军医大学：2.西京医院心脏外科，3.生理学教研室，4.西京医院核医学科，陕西 西安 710032)
Author(s):
GU Lan-xin1;  WANG Zhi-fa2; 3;  YIN Qiang2;  WANG Yun-ya4
(1.Xi’an Gaoxin International High School, Xi’an 710075, Shaanxi, China;
2.Department of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China;
3.Department of Physiology, School of Basic Medical Sciences, Fourth Military Medical University, Xi’an 710032, Shaanxi, China;
4.Department of Nuclear Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
慢性睡眠剥夺; 胰岛素抵抗; 有氧运动; 炎症因子; 大鼠
Keywords:
Chronic sleep deprivation;  insulin resistance;  aerobic exercise;  inflammatory cytokines;  rat
分类号:
R359.2
DOI:
-
文献标识码:
A
摘要:
目的 观察有氧运动对慢性睡眠剥夺（CSD）引发胰岛素抵抗(IR)的影响，并明确其可能机制。方法 采用改良多平台水环境睡眠剥夺法(MMPM)对大鼠进行为期8周的睡眠剥夺建立CSD模型。雄性Sprague-Dawley大鼠30只，随机分为5组：对照组、睡眠剥夺组、睡眠剥夺+低强度运动组、睡眠剥夺+中强度运动组、睡眠剥夺+高强度运动组。通过测定空腹血清胰岛素、空腹血糖、胰岛素抵抗指数(homeostasis model assessment for insulin resistance,HOMA-IR)、心肌葡萄糖摄取、腹腔糖耐量和空腹胰岛素敏感性等手段观察有氧运动对CSD引发大鼠IR的影响；采用彩色多普勒超声诊断仪检测短轴缩短率(Fractional Shortening,FS)及左心室射血分数(Left Ventricular Ejection Fraction, LVEF)；采用放射免疫法测量血清中肿瘤坏死因子（tumor necrosis factor，TNF）-α、白介素(interleukin，IL)-1β、IL-6水平；通过Western blot法检测外周血单核细胞核因子（NF-kappa B，NF-κB）以及抑制蛋白（I-kappa B，I-κB），观察有氧运动对慢性睡眠剥大鼠体内炎症因子水平及心功能的影响。结果 CSD可引起大鼠HOMA-IR、腹腔糖耐量和胰岛素敏感性的明显异常，降低心肌葡萄糖摄取和心脏功能，同时可降低抑炎因子I-κB水平，增加促炎因子NF-κB和血浆炎症因子水平（P<0.05）。低强度运动干预对上述各指标无明显影响；而中强度运动干预则可明显改善大鼠机体IR、心肌IR及心脏射血功能，增加抑炎因子I-κB，降低机体促炎因子NF-κB以及外周各炎症因子的水平（P<0.05）；但高强度运动干预则可引起机体IR加重、心肌葡萄糖摄取能力下降及心脏射血分数下降，体内促炎因子NF-κB以及外周各炎症因子水平升高，而抑炎因子I-κB表达水平下降（P<0.05）。结论 适量有氧运动可以通过降低体内炎症反应改善大鼠睡眠剥夺引发的IR及心功能下降。
Abstract:
AIM To explore the effect of aerobic exercise on insulin resistance induced by sleep deprivation and the underlying mechanisms. METHODS A modified multiple platform method was used in a chronic sleep deprivation (CSD) model in rats. Thirty male 8-week-old Sprague-Dawley rats were randomized into a control group, CSD group, CSD with low-intensity aerobic exercise training (CSD+L) group, CSD with moderate intensity exercise training (CSD+M) group and CSD with high-intensity exercise training (CSD+H) group (n=6 per group). The intraperitoneal glucose tolerance test (IPGTT), insulin tolerance test (ITT), positron emission tomography (PET-CT) and insulin resistance index (HOMA-IR) were employed and the levels of IL-1β, IL-6, TNF-α and I-kappa B (I-κB) and NF-kappa B (NF-κB) in peripheral blood mononuclear cells (PBMCs) were assessed. Shortening fraction (FS) and left ventricular ejection fraction (LVEF) were measured by echocardiography after 8-week chronic sleep deprivation in all the groups with or without exercise. RESULTS Compared with those in the control group, CSD decreased HOMA-IR, insulin sensitivity and glucose tolerance as well as peripheral inflammation inhibitory cytokine (Iκ-B), reduced myocardial 18FDG uptake and LVEF but increased proinflammatory cytokine NF-κB. Compared with those in the CSD group, CSD+L resulted in no significant changes in the above indexes. CSD+M markedly lowered CSD induced insulin resistance, decreased the level of peripheral inflammatory cytokines and proinflammatory cytokine NF-κB and increased and myocardial 18FDG uptake and LVEF. CSD+H increased insulin resistance, serum inflammatory cytokines and proinflammatory cytokine NF-κB and decreased peripheral inflammation inhibitory cytokine Iκ-B, myocardial 18FDG uptake and LVEF. CONCLUSION CSD induced significant insulin resistance and systematic inflammation; however, moderate, not low or high, intensity aerobic exercise of aerobic exercise, improved insulin sensibility, at least partially by suppressing the serum inflammatory cytokines in response to CSD, indicating a proper intensity of aerobic exercise could improve LVEF and insulin resistance induced by chronic sleep deprivation.

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备注/Memo

备注/Memo:
收稿日期：2017-03-13.基金项目：国家自然科学基金项目资助（81370352）
通讯作者：王云雅，副主任技师，主要从事心血管疾病干细胞治疗研究 Email：wangyunyaxj@163.com
作者简介：顾兰馨，高中生 Email：2839871189@qq.com 共同第一作者：王志法，住院医师，硕士生 Email：1127101384@qq.com
更新日期/Last Update: 2017-06-06