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|本期目录/Table of Contents|

Obestatin对慢性心力衰竭大鼠心功能及尿量的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2018年第1期
页码:
9-012
栏目:
老年心血管病研究
出版日期:
2017-10-10

文章信息/Info

Title:
Influence of obestatin on cardiac function and urine volume in chronic heart failure rats
作者:
石建波1; 周勇1; 姚均迪1; 郭志福2; 郑兴2
（1.解放军第411医院心内科，上海 200083；2.第二军医大学附属长海医院心内科，上海 200433）
Author(s):
SHI Jian-bo1;  ZHOU Yong1;  YAO Jun-di1;  GUO Zhi-fu2;  ZHENG Xing12
(1.Department of Cardiology, PLA 411 Hospital, Shanghai 200083, China; 2.Department of Cardiovascular Diseases, Changhai Hospital, Second Military Medical University, Shanghai 200433, China)
关键词:
Obestatin; 慢性心力衰竭; 心功能; 尿量; 大鼠
Keywords:
obestatin;  chronic heart failure;  cardiac function;  urine volume;  rats
分类号:
R541.6
DOI:
-
文献标识码:
A
摘要:
目的 观察Obestatin腹腔注射对前壁心梗致慢性心衰（CHF）大鼠模型的心功能及其尿量的影响。方法 36只SD大鼠随机分为6组:对照组（n=6）及CHF模型组（n=30），后者包括：假手术组、精氨酸加压素（AVP）组、生长激素释放肽（Ghrelin）组、低剂量Obestatin组、高剂量Obestatin组（n均=6）。通过结扎心脏前降支建立CHF大鼠模型。16周后行心脏超声检查，并收集各组大鼠的24 h尿量，随后每天1次腹腔注射：对照组注射生理盐水，其余5组大鼠分别注射生理盐水、AVP、Ghrelin以及低剂量Obestatin〔50 μg/(kg·d)〕和高剂量Obestatin〔100 μg/(kg·d)〕，注射4周后复查心脏超声并收集各组大鼠当天24 h尿量。结果 腹腔注射4周后，与注射前相比，高剂量Obestatin组大鼠心脏超声左心室射血分数（LVEF）显著升高，尿量显著增多(均P<0.05)；AVP组大鼠LVEF显著下降(P<0.01)，尿量显著下降(P<0.05)；Ghrelin组大鼠心脏超声LVEF值显著升高(P<0.01)，尿量无显著变化；对照组、生理盐水组、低剂量Obestatin组大鼠LVEF、尿量无显著变化。结论 高剂量Obestatin长期腹腔注射能明显改善心梗后心衰大鼠心功能，增加尿量。
Abstract:
AIM The present investigation studied the effects of obestatin on changes in cardiac function and urine volume in post infarction chronic heart failure (CHF) rats. METHODS Thirty-six rats were randomly equally divided into 6 groups. Five CHF groups were established by ligating the left anterior descending artery (LAD), whereas rats in the sham group underwent chest operation without LAD ligation. After a 16-week normal diet, left ventricular ejection fraction (LVEF) was measured by echocardiography and a 24-hour urine was collected to measure urine volume in all sham and CHF rats. RESULTS The sham group was given intraperitoneal injection (IP) of saline, while the other five CHF groups were given IP of saline, AVP, ghrelin, obestatin 50 μg/(kg·d) and obestatin 100 μg/(kg·d), respectively. After the 4-week IP, LVEF was measured by echocardiography and a 24-hour urine was collected to measure urine volume in all sham and CHF rats. Compared with baseline, after the 4-week treatment, LVEF and urine volume increased significantly in the obestatin 100 μg/(kg·d) treatment group (P<0.05). LVEF decreased (P<0.01) and the urine volume also decreased significantly (P<0.05) in the AVP treatment group. LVEF increased significantly (P<0.01) but there were no significant changes in urine volume in the Ghrelin treatment group. There was no significant change in LVEF or urine volume in the sham group given saline or the CHF group given saline or the CHF group given obestatin (50 μg/kg·d). CONCLUSION In summary, high dose IP of obestatin, in a dose of 100 μg/(kg·d), improves cardiac function and urine volume in surgically prepared CHF rats.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2017-08-22.通讯作者：姚均迪，主任医师，主要从事老年心血管病研究 Email：yaojundi411@126.com 作者简介：石建波，主治医师，博士 Email：stevenshi12@126.com
更新日期/Last Update: 1900-01-01