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|本期目录/Table of Contents|

关附甲素对单个心肌细胞钠、钙通道的阻断作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
1998年第4期
页码:
225-226,229
栏目:
论著
出版日期:
1998-07-01

文章信息/Info

Title:
Blocking effects of guanfu base A on sodium channel and L - type calcium channel in single ventricular myocytes
作者:
裴德安
无锡市第二人民医院心内科 214002
Author(s):
Pei Dean
Department of Cardiology, Second People′s Hospital of Wuxi,Wuxi 214002
关键词:
关附甲素　膜片钳　离子通道　心室肌细胞　豚鼠
Keywords:
guanfu base A 　patch clamp 　ion channel 　ventricular myocyte　guinea pig
分类号:
-
DOI:
-
文献标识码:
-
摘要:
用膜片钳全细胞记录法研究关附甲素(GA) 对分离的单个豚鼠心室肌细胞钠通道电流(INa) 和L-型钙通道电流(ICa-L) 的影响。结果表明: 8.40 umol/LGA 使INa峰值( INamax) 从8.02±2.53 nA 分别降至5.79±1.82 nA 和4.44±1.81 nA (n=8, P < 0.05 和P <0.01) ; 使ICa-L最大峰值( ICa-Lmax ) 从1020.8±197.3 pA 分别降至523.0±101.8pA和429.6±120.0 pA (n=5, 均P <0.01)。GA 使INa和ICa-L的电流-电压曲线上移, 但均不改变其激活、峰值和反转电位。本研究表明: GA 对INa和ICa-L具有浓度依赖性阻滞作用, 这种阻滞作用可能为其抗心律失常作用的主要机制。
Abstract:
The effects of Guanfu base A (GA) on sodium channel current INa) and L-type calcium channel current (ICa-L) were investigated in isolated single ventricular cells from guinea pig hearts by means of patch clamp whole-cell recording. The results showed that GA at 8, 40 umol/L decreased peak INa(Iinf>Namax) from 8.02±2.53 nA to 5.79±1.82 nA and 4.44±1.81 nA (n=8, P <0.05 and P <0.01) , reduced peak ICa-L( ICa-Lmax ) from 1020.8±197.3 pA to 523.0±101.8 pA and 429.6±120.0 pA (n= 5, P < 0.01). GA shift up boh the I-V curve of INa and that of ICa-L, but didn′t change their active, peak and reverse potentials. It reveals that GA blocks both INa and ICa-Lin a concentration dependent manner, which might be the major mechanism of its antiarrhythmic action.

参考文献/References

［1］刘静涵, 王洪城, 高耀良, 等. 中国乌头之研究--X VI 关白附子中的新生物碱(2). 中草药, 1981; 12 (3) : 1.

［2］陈维洲, 董月丽, 张月芳, 等. 关附甲素的抗心律失常作用. 中国药理学报, 1983; (4) : 247.

［3］周礼明, 徐济明, 陈祥华, 等. 关附甲素对豚鼠心室乳头状肌动作电位的电生理效应. 中国药理学报, 1991; 12 (5) : 465.

［4］Kazuto Y, Muneshige K, Masahiro O , et al. An improved method for isolating cardiac myocytes useful for patch-clamp studies. J pn J Physiol, 1990; 40: 157.

［5］Hamill OP,Marty A ,Neher E, et al. Improved patch-clamp techniques for high-resolution current recording from cells and cellfree membrane patches. Pfluegers Arch, 1981; 391: 85.

［6］Beller GW , Reuter H. Voltage clamp experiments on ventricular myocardial fibers. J Physiol (L ond ) , 1970; 207: 165.

[7]Mitra R,Morad M. Two types of calcium channels in guinea pig ventricular myocytes. Proc Natl Acad Sci USA , 1986; 83: 5340.

[8]Josephson IR, Sanchez-Chapula J , Brown AM. Early outward current in rat single ventricular cells. Circ Res, 1984; 54: 157.

[9]Belles B,Malecot CO, Hescheler J , et al. “Run dowm ”of Ca2+ current during long time whole-cell recording in guinea pig heart cells: Role of phosphorylation and intracellular calcium. Pfluegers Arch, 1988; 411: 353.

[10] Sanchez-Chapula J , Tsuda Y, Josephson IR. Voltage and use dependent effect of lidocaine on sodium current in rat single ventricular cells. Circ Res, 1983; 52: 557.

备注/Memo

备注/Memo:
(收稿1997-12-26)
更新日期/Last Update: 1998-07-01