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定量反转录PCR检测血管紧张素Ⅱ的1型受体内标准的构建(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2000年第6期
页码:
432-434
栏目:
论著
出版日期:
2000-12-01

文章信息/Info

Title:
Synthesis of internal standard for detecting expression of angiotensin Ⅱ type 1 receptor by quantitative reverse transcription polymerase chain reaction
作者:
万发银1;  汪家瑞1;  夏宁邵2;  张　军2;  曾　定2;  陈　福3
1. 首都医科大学宣武医院心内科, 北京100053; 2. 厦门大学肿瘤细胞工程国家专业实验室; 3. 厦门大学抗癌中心
Author(s):
WAN Fa-yin;  WANG Jia-rui;  XIA Ning-shao;  ZHANG Jun;  ZENG Ding;  CHEN Fu
Department of Cardiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
关键词:
受体;  血管紧张素;  定量RT-PCR;  标准曲线
Keywords:
receptors;  angiotensin;  quantitative reverse transcription polymerase chain reaction;  standard curve estimat
分类号:
Q52
DOI:
-
文献标识码:
A
摘要:
作者利用 Balb/ c小鼠的血管紧张素 的 1型受体(AT1 )基因和兔 AT1 基因的序列的不同 ,合成了一个新的含有两种动物序列的内标准 RNA,有效地消除了二级结构对反转录 PCR(RT- PCR)的影响。本研究对利用这种内标准 RNA进行定量 RT- PCR进行初步评价。
Abstract:
In this test,we synthesized an internal standard RNA,which have part sequences of receptor type 1 of ATⅡ (AT 1) gene of the Balb/c mouse and rabbits,utilizing difference of the AT 1 gene sequence in these two animals,This internal standard RNA abolishes the influence of the secondary struture in the reverse transcription polymerase chain reaction (RT PCR). In the standard curve estimation by the quantitative RT PR using this internal standard RNA,the correlation coefficient is 0.998. From above data,it is suggested that the AT1 expression detecting by quantitative RT-PCR using this internal standard RNA can be truly reflected and the quantitative RT-PCR using this internal standard RNA is a credible method. Moreover, this internal standard RNA can be made simply and speedily.

参考文献/References

[1] Sadoshima J, Lzumo S. Molecular characterization of angiotensin II-induced hypertrophy of cardiac myocytes and hyperplasia of cardiac fibroblasts. Critical role of the AT1 receptor subtype[J]. Circ Res, 1993, 73 (3) : 413.

[2] Li JS, Touyz RM, Sch iffrin EL. Effects of AT1 and AT2 angiotensin receptor antagonists in angiotensin II-infused rats[J]. Hpertension, 1998, 31: 487.

[3] Kim S, Ohta K, Hamaguchi A, et al. Angiotensin II type 1 receptor antagonist inhibits the gene expression of transforming growth factor-beta 1 and extracellular matrix in cardiac and vascular tissues of hypertensive rats [J]. J Pharmacol Exp Ther, 1995, 273: 509.

[4] Kim S, Lwao H. Involvement of angiotensin II in cardiovascular and renal injury: effects of an AT1-receptor antagonist on gene expression and the cellular phenotype [J]. J Hypertens Suppl, 1997, 15: S3.

[5] Ju H, Zhao S, Jassal DS, et al. Effect of AT1 receptor blockade on cardiac collagen remodeling aftermyocardial infarction[J]. Cardiovasc Res, 1997, 35: 223.

备注/Memo

备注/Memo:
收稿日期：1999-09-28.基金项目：厦门大学肿瘤细胞工程实验室开放基金的资助
更新日期/Last Update: 2000-12-01