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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2001年第1期

页码:

1-4

栏目:

论著

出版日期:

2001-01-25

文章信息/Info

Title:

The m echanisms underlying the effects of acetylch0line on isolatedguinea—pig ventricular myocytes

作者:

方萍¹; 减伟进¹; 于晓江¹; 张晓东²; 臧益民²

1.西安交通大学医学院心血管生理药理研究室，陕西西安710061； 2.第四军医大学生理学教研室

Author(s):

FANG Ping¹; ZANG Wei fin¹; Yu Xiao jiang¹; Zhang Xiae dong²; ZANG Yimin²

1．Division at Cardiovascular Physiology＆．Pharmacology．School of Medicine，Xi an Jiaotong University，Xi an，Shaanxl 710061，China．2．Department of Physiology，Fourth M ilitary Medical Universit

关键词:

乙酰胆碱; 心室肌; 动作电位; 收缩力

Keywords:

acetylcholine; ventricular myocytes taction potential; foroe contraction

分类号:

R963

DOI:

-

文献标识码:

A

摘要:

目的 研究己酰胆碱(ACh)对离体豚鼠心室肌的直接负性作用及机制。方法 采用标准玻璃散电极细胞内记录技术记录动作电位(AP)及肌力换艟器记录心肌收螭力(Fc)的方法观察ACh对高体豚鼠心室肌的作用，井观察几种受体或通道水平的阻断荆阿托品、氯化铯(CsCI) 氯化镉(cdC1t)对ACh直接作用的影响。结果 10 mol／LACh对心室肌动作电位持续时『目I(APD)及FC的抑制率分别为7 3l 和37．57 <0 05)．10 tool／L阿托品和2O mmol／L CsCl可阻断该作用，0．1 mmol／L cdc12对该作用无影响。结论 10 tool／L ACh对离体琢鼠心室肌有直接负性作用．ACh的作用与毒草碱型胆碱受体及K 电流有关．而与ca抖电流的关系可能不大。

Abstract:

AIM To investigate the mechanisms underlying the effects of ACh on isolated guineapig ventricular myocytes． METHODS Using standard microelectrodes and force transducer we recorded the effects of ACh (10一mol／I，)and various blockers such ns atropine，CsC1 and CdC1，on the action potentia1 and force contraction in isolated ventrlcular myocytes of guinea—pig．RESULTS ACh(10～ mol／i，)reduced the duration of action potential and twitch tensions by 7．3l and 37．57 respectively (P< 0．05)，which could be blocked by atropine，CsCI，but not by CdCl2．CONCLUSION ACh(10一mol／I )could cause a negative chronotropic and inotropic effects on guinea—pig ventricular myocytes．These effects are related to the muscarinic receptor and the potassium channel and may not related to the calcium channe1．

参考文献/References

[1]臧伟进，臧益民．乙酰胆碱对心脏的作用及离子机制[J]．心功能杂志，1993，5（3）：194．

[2]徐江平，童国明，刘传馈．毒草碱样己酰胆碱受体的分子药理学研究进展．金正均，王永铭，苏定冯．药理学进展[M]．第3版．上海，科学出版杜．1999．232—241.

[3]Creme T1FH，TeismmnACH VangilstwH．et a1．Use of microdialysis for monitor sympathetic mad parasympathedc inner—radon of heart in conscious rats[J] Am．J，Physiol，J997，273：H2850．

[4]Boyett MR．Kirby MS，Orchard CH．et a1．The negative in—ntmpio effect acetylcholine on ventrioular myocardium[j] ．J，Physiol，l988，4O4: 6l3．

[5]McmcnSO，Harrison SM．ZangWJ，et a1．A direct negative inotropic effect 0f acetylcb,oIine 0n rat ventricular myocytes [J]．Am ．J，Physio1．1993，265，H1393．

[6]Sharma VK，Colecraft HM，Rubin I．E．et al．Does mammalianhean contain only,the M2 muscsrinic receptor subtype[J] lifeSci,l997，60(13-14)：1023.

[7]Kim Modulation of acetylchofine·activated K channel func．tiott in rBt atrial cells by phosphorylafion[J]、J Phasing．1991．437t133．

[8]Boyett MR， bem A．The fade 0f the respcnse t0 acety1． choline at the rabbit isolated inwatrial node[J]．J．Physiol，1987．393, 171．

[9]Koumi S1，Waserstrrom JA．AcetyIchol Jne-semitiv muscarinicK ckarmels mammalian ventxicular myocyt [J] ．Am，JPhysiol ，1994，266：HJ812.

备注/Memo

备注/Memo:

收稿日期：2000-11-21.基金项目：国家自然科学基金资助项目(No．39870334；No．39970273)

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更新日期/Last Update: 2001-01-25