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失血性休克大鼠心脏G蛋白变化及纳络酮对它的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第2期
页码:
117-119
栏目:
论著
出版日期:
2001-03-01

文章信息/Info

Title:
Changes of the contents of G protein on hemorrhagic shock of rats and the effect of naloxone
作者:
熊仁平; 周元国; 刘苹
第三军医大学大坪医院野战外科研究所分子生物学中心，重庆400042
Author(s):
XIONG Ren-ping; ZHOU Yuan-guo; LIU Ping
Molecular Biologic Center，Research Institute of Surgery and Daping Hospital，Third Military Medical University．Chongqing 400042，China
关键词:
G-蛋白; 休克; 出血性; Western blot; 太鼠; 心脏组织; 纳络酮
Keywords:
G protein; shock; hemorrhagic; naloxone; western blot; ventricle; rat; naloxone
分类号:
R362
DOI:
-
文献标识码:
A
摘要:
目的 研究失血致低血容量性休克大鼠心脏组织鸟苷酸结合蛋白(Guanine nucleotide-binding protein．G 蛋白)含量的变化及纳络酮对其影响。方法 颈总动脉放血，使平均动脉压降至45mm Hg(1mm Hg=0．133 kPa)，稳定1 h，完成失血致低血容量性体克大鼠模型，21只大鼠随机均分为：休克组、纳络酮治疗组、空白对照组。采用Western blot技术测定大鼠体克后6 h心脏组织中Gsa，Gia，Gqa，Goa的含量．结果 休克后心脏膜组织Gsa两条带较对照组显著降低(P
Abstract:
AIM To study the alteration of the content of guanine nucleotide-binding protein a subunits (Ga protein)in myocardium during shock，and the effect of naloxone (NAL)on the G protein．METHODS Hemorrhagic shock was performed in Wistar rats by carotid artery bleeding to maintain the mean arterial pressure(MAP)at 45 mm Hg (1 mm Hg= 0．133 kPa)for one hour Twenty-one Wistar rats were randomly divided into three groups．Group 1(NS,n=7)，as control；Group 2(HS n=7),shock control；Group 3(NAL，n=7),NAL+HS．Determining mean arterial pressure,then，the content of Gsa，Gis, Gqa, Goa in the sarcolemma were measured by Western blot analysis at 6 hours after shock．RESULTS In the ventricles，the contents of two Gsa bands,one Goa band in the group HS were significantly lower than in the group NS，P< 0.05，P< O．01 and P< 0．05．But，the content of two Gsa bands in groups NAL were significantly higher than in the group HS,P< 0．01；P< O．01．The content of one Goa hand in the groups NAL was significantly lower than in the group HS,P< O．01．The content of one Gin band and Gqa band in the group Hs was significantly higher than in the group NS,P< 0.01 and P< 0.05.The lower in the conent of one Gia band and Gqa band were attenuated in the groups NAL as compared with the group HS． CONCLUSION Alteration of content of G proteins in the ventricles may contribute to the dysfunction of adenyl cyclase signal transduction system during the hemorrhagic shock．NAL not only could improve hemorrhagic shock，but also could change the level of G protein．

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2000-02-01.
更新日期/Last Update: 2001-03-01