用于离子通道基因表达的爪蟾卵母细胞的分离和培养(PDF)
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2001年第2期
- 页码:
- 120-122
- 栏目:
- 论著
- 出版日期:
- 2001-03-01
文章信息/Info
- Title:
- An improved method of the preparation of Xenopus oocytes for gene expression of ion channels
- Author(s):
- ZHANG Xiao-dong; ZANG Yi-min; XIE An
- Department of Physiology,Fourth Military Medical University．Xi'an，Shaanxi 710032，China
- Keywords:
- Xenopus oocytes ; ion channels; nL injection
- 分类号:
- Q813.11
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 应用非洲爪蟾卵母细胞对外源性离子通道基因进行表达是目前离子通道结构和功能研究中的一种重要的方法．它实现了分子生物学和电生理实验的很好结合。作者基于离子通道研究的实验需要，提出了一种用于离子通道基因表达的卵母细胞的制备方法。
- Abstract:
- It is a very important method to study the correlation between the structure and the function of ion channels by the Xenopus oocyte expression system now，which leads to the perfect combination of molecular biology and e1ectrophysio1ogy experiment．This paper presents an improved method of preparing Xenopus oocytes for ion channel gene expression.
参考文献/References
[1]King RC．Handbook of Genetics [M]．New York,Plenum Press, 1975.
[2]Gurdon JB,Lane CD,Woodland HR， et a1．Use of frog eggs and oocyte for the study of messenger RNA and its translation in living cells[J]．Nature，1971,233:177-182．
[3]Goldin AL．Maintenance of Xenopus laevis and oocyte injection [A]．Rudy B，Iverson LE.Methods in enzymology：ion channel [M]．Californla：Academic Press，1992,267-279．
[4]Stuhmer W,Parekh AB．ELectrophysiological recordings from Xenopus oocytes[A]. Sakmann B，Neher E．Single channel recording[M]．Second edition．New York：Plenum Press．1995,341-453．
备注/Memo
- 备注/Memo:
- 收稿日期：2000-11-01.
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更新日期/Last Update: 2001-03-01