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p27蛋白表达对精氨酸加压素介导心脏成纤维细胞增殖的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第2期
页码:
122-124，127
栏目:
论著
出版日期:
2001-03-01

文章信息/Info

Title:
Role of p27 in the proliferation of cardiac fibroblasts modulated by arginine vasopressin
作者:
陈永清; 赵连友; 彭育红; 范延红; 周秀荣; 徐琳
第四军医大学唐都医院心内科，陕西西安710038
Author(s):
CHEN Yong-qing; ZHAO Lian-you; PENG Yu-hong; FAN Yan-hong; ZHOU Xiou-rong;  XU Lin
Department of Cardiology， Tangdu Hospita1, Fourth Military Medical University， Xi'an Shaanxi 710038，China
关键词:
蛋白质p27; 精氨酸升压素; 成纤维细胞; 心脏; 增殖
Keywords:
protein p27; argipressin; fibroblasts; cardiac; proliferation
分类号:
Q71；R54
DOI:
-
文献标识码:
A
摘要:
目的 观察精氨酸加压素(AVP)作用下 p27蛋白表达在心脏成纤维细胞(CFs)中的变化．探讨p27蛋白调控AVP促CFs增殖的作用。方法 以培养的新生SD大鼠CFs为实验动物模型，采用胰酶消化、差速贴壁法培养CFs，四氮唑盐(MTT)比色法检测细胞增殖，碘化丙啶标记细胞DNA，p27蛋白的单抗和标记了FITC的二抗标记细胞内的p27蛋白，采用流式细胞分析仪技术测定细胞周期及p27蛋白表达的阳性率。结果 ①随AVP浓度的增加，MTT法测得的CFs的A值呈递增趋势，其中10-7，10-6 mol／L AVP组分别为0．307土0．009，0．337土0．007，均明显地高于对照组(0．298+0．009)．并有统计学意义(分别P<0.05．P<0．01)；②随AVP浓度的增加，CFs的S期细胞百分率和细胞增殖指数(PI)逐渐增加，Go／G1期百分率下降，10-7，10-6 mol／L AVP组与对照组比较差异非常显著(P<0．01)；③CFs的p27蛋白表达阳性率随AVP浓度的增加而呈递减趋势．其中1O-7，lO-6mol／L AVP组(分别为71.6% 土 2．2%,63．1%土2．3 %)明显低于对照组(86.5% 土2．3%)，并有统计学意义(P<0．01). 结论 p27蛋白是AVP促CFs增殖过程的重要负性调节因子，AVP通过下调p27蛋白发挥促CFs增殖作用，这可能是AVP致心脏纤维化的一种分子机制。
Abstract:
AIM To study the change of p27 expression in cardiac fibroblasts(CFs)proliferation，investigate the role of p27 in the CFs proliferation modulated by arginine vasopressin(AVP)．METHODS As a model，neonatal rat CFs was cultured with enzymatic dissociation．AVP in different concentration was added．Proliferation was measured through MTT assay．To identify the cell cycle，propidium iodide was used to lable DNA．Cell cycle distribution and p27 expression were determined with flow cytometre．RESULTS AVP increased A value of CFs in a concentration dependent manner．The value of 10-7 and l0-6 mol／L AVP groups by MTT assay were 0．307士0．009 and 0．337士0．007 respectively，which were significantly higher than that of the control (O．298士0．009)(P< 0．05，P< 0．01，respectively)．The percentage of CFs in S stage and proliferation index (PI)were increased significantly with the elevation of AVP concentration,concomitant with a significant decrease in the percentage of G。/Gl stage cells. Positive rate of p27 expression in CFs was downregulated by AVP also in a concentration dependent manner． Positive rate of p27 expression in CFs of 10-7 and l0-6 mol／L AVP groups(71．6%士2．2%，63.1%士2．3%)were significantly lower than that of the control (86．5%士2．3%)(P< 0.01).CONCLUSION p27 may be a negative modulating factor in CFs proliferation．By downregulating p27 expression，AVP can increase CFs proliferation，thus，participate in the pathogenesis and development of cardiac fibrosis．

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2000-09-11.
更新日期/Last Update: 2001-03-01