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乙酰胆碱对离体豚鼠心房肌动作电位及其脱敏的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第3期
页码:
165-169
栏目:
论著
出版日期:
2001-05-01

文章信息/Info

Title:
The effect of acetylcholine on desensitization of action potential in guinea-pigs atrial muscles
作者:
张凤杰1; 臧伟进1; 于晓江1; 马志一1; 臧益民2
1西安交通大学医学院心血管生理药理研究室，陕西 西安 710061;2 第四军医大学生理学教研室;
Author(s):
Zhang Feng-jie 1; Zang Wei-jin 11; Yu Xiao-jiang1; Ma Zhi-yi1;  Zang Yi-ming2
1 Division at Cardiovascular Physiology & Pharmacology, Xi'an Jiaotong University, Xi'an Shaanxi 710061, China; 2 Department of Physiology, Fourth Military Medical University,Xi'an Shaanxi 710032, China
关键词:
乙酰胆碱;  心肌;  心房;  动作电位;  脱敏
Keywords:
acetylcholine; myocardium; Heart atrium; action potential duration; desensitization
分类号:
R331.38
DOI:
-
文献标识码:
A
摘要:
目的 研究迷走神经递质乙酰胆碱(ACh)对离体豚鼠心房肌细胞动作电位(AP)的影响及其对心房肌的脱敏,探讨脱敏可能发生的机制.方法 采用标准玻璃微电极细胞内记录方法,观察不同浓度(0.01,0.1,1 μmol/L) ACh对心房肌细胞AP的影响及对动作电位时程( APD)和收缩力的脱敏现象,并观察了受体或通道水平的阻断剂阿托品、氯化铯 (CsCl)对ACh所致的心房肌APD脱敏的影响.结果 ①0.01,0.1,1 μm ol/L ACh分别缩短APD的变化率为(6.02±1.04)%,(14.20±3.79)%,(30.20±3.65)%.②0. 01μm ol/L ACh对心房肌细胞没有脱敏;0.1 μmol/L ACh对心房肌细胞APD有轻微脱敏,脱敏持续时间为1 min;而1 μmol/L ACh对心房肌细胞APD脱敏明显,脱敏持续时间为5 min.③1 μ mol /L阿托品与20 mmol/L CsCl并不能阻断1 μmol/L ACh对心房肌细胞APD的脱敏.结论 ACh缩短 APD的作用随浓度增大而增大;ACh对离体豚鼠心房肌细胞APD的脱敏有浓度依赖性与时间依赖性;脱敏的发生可能与毒蕈碱型胆碱能受体及Ik,ACh电流有关.
Abstract:
AIM To investigate the mechanism underlying the effects of ACh on desensitization of action potential in guinea pigs atrial muscles. METHODS Using standard microelectrodes and force transducer we recorded the effects and desensitization of ACh (0.01,0.1,1μmol/L) and blockers (such as atropine, CsCl) on the action potential duration(APD) and force contraction in atrial muscles. RESULTS ①0.01,0.1,1μmol/L ACh shortened the APD by (6.02±1.04)%,(14.20±3.79)% and (30.20±3.65)% respectively. ②0.01μmol/L ACh has no desensitization and the time of desensitization at 0.1 and 1μmol/L ACh lasted 1min and 5min, respect ively. ③The desensitization at 1μmol/L Ach could not be blocked by 1μmol/L atropine, 20 mmol/L CsCl. But atropine or CsCl could decrease the effect of shortening APD caused by ACh. CONCLUSION The effect of desensitization on APD to ACh in atrial cells is concentration and time-dependent, these effects maybe related to the change of the muscarinic receptor and the potassium channel.

参考文献/References

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[2] 臧伟进, 臧益民. 心脏对乙酰胆碱的脱敏现象[J]. 心功能杂志,1993, 5 (4) : 284.

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备注/Memo

备注/Memo:
收稿日期：2000-09-12.基金项目：国家自然科学基金资助课题!( No.39870334 ;39970273 )
更新日期/Last Update: 2001-05-01