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胰岛素促进在体犬心肌GLUT1移位及其意义(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第3期
页码:
178-179, 182
栏目:
论著
出版日期:
2001-05-01

文章信息/Info

Title:
Insulin stimulates translocation of GLIT1 and glucose uptake in canine myocardium in vivo
作者:
殷仁富1;  陈金明1;  吴宗贵1;  仇韶华1;  王咏梅1;  孔宪涛2
第二军医大学长征医院: 1. 心内科, 2. 实验科, 上海200003
Author(s):
YIN Ren-fu1;  CHEN Jin-ming1;  WU Zong-gui1;  QIU Shao-hua1;  WANG Yong-mei1;  KONG Xian-tao2
1 Department of Cardiology, Changzeng Hospital, Second Military Medical University, Shanghai 200003, China
关键词:
胰岛素;  心肌细胞;  葡萄糖;  葡萄糖转运子1
Keywords:
insulin;  myocardium;  glucose;  GLUT1
分类号:
Q591.4
DOI:
-
文献标识码:
A
摘要:
目的 观察胰岛素能否刺激心肌葡萄糖转运子 1(GL UT1)移位和葡萄糖摄取。方法 利用自动分析仪测定生理代谢参数 ,应用免疫印迹和免疫荧光法检测 GL UT1。结果 胰岛素使心肌细胞质膜 GL UT1增加 [从 (42± 5 ) %至 (6 3± 8) % ,P<0 .0 5 ]。细胞器膜 GL U T1则相应减少 ,同时伴随葡萄糖摄取量增加 (P<0 .0 1)。结论 胰岛素刺激引起 GL UT1移位 ,使心肌葡萄糖摄取增加。应用胰岛素有助于增加心肌葡萄糖的摄取和利用。
Abstract:
AIM To investigate whether insulin stimulates the translocation of GLUT1 and glucose uptake in canine myocardium. METHODS Physiological metabolic parameters were determined by autoanalyser and GLUT1 was studied by immunoblot analysis. RESULTS Insulin increased GLUT1 significantly in sarcolemma of canine myocardium [(42±5)% vs (63±8)%, P <0.05],but decreased the GLUT1 content in intracellular membrane. And insulin also increased the myocardiol glucose uptake, as compared with the baseline. CONCLUSION Insulin stimulation results in GLUT 1 translocation and then increases glucose up take in canine myocardium. Insulin helps to increase the up take and utilization of myocardial glucose.

参考文献/References

[1] Marette A. Richardson JM, Ramlal T, et al. Abundance, localization, and insulin-induced translocation of glucose transporters in red and white muscle [J]. Am J Physiol, 1992, 263: C443-C452.

[2] Shi CQ, Sinusas AJ, Dione DP, et al. Technetium-99-M-nitroimidazole (BMS 181321): a positive imaging agent for detecting myocardial ischemia [J]. J Nuci Med , 1995, 36 (6): 1078-1086.

[3] 殷仁富, 胡晓月, 仇韶华, 等. GLUT4 移位是缺血心肌增加葡萄糖摄取的调节机制[J]. 第二军医大学学报, 1999, 20 (增刊) :1056- 1058.

[4] Egert S, Nguyen N, Brosius III FC, et al. Effects of wortmannin on insulin-and ischemia-induced stimulation of GLUT4 translocation and FDG up take in perfused rat hearts [J]. Cardiovasc Res, 1997, 35: 283- 293.

[5] Young LH, Renfu Y, Russell R, et al. Low-flow ischemia leads to transloeation of Canine heart GLUT4 and GLUT1 glucose transporters to the Sarcolemma in vivo[J]. Circulation, 1997, 95: 415- 422.

[6] 殷仁富, 胡晓月, 仇韶华, 等. 低血流心肌缺血引起犬心肌葡萄糖转运蛋白向细胞膜移位[J]. 中华心血管病杂志, 1999, 27(6) : 428.

[7] Fischer Y, Thomas J , Sevilla L, et al. Insulin-induced recruitment of glucose transporter 4 (GLTU 4) and GLUT1 in isolated rat cardiac myocytes[J]. J Biol Chem, 1997, 272: 7085- 7092.

[8] Tsakiridis T, Vranic M, Klip A. Phosphatidylinositol 3-k inase and the actin network are not required for the stimulation of glucose transport caused by mitochondrial nucoupling: camparison with insulin action[J]. Biochem J , 1995, 309: 1- 5.

备注/Memo

备注/Memo:
收稿日期：2000-09-18.
更新日期/Last Update: 2001-05-01